Regulation of phenobarbital-induced ferrochelatase mRNA activity by dibutyryl cAMP and glucose in normal and diabetic rat hepatocytes.

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The induction of ferrochelatase activity by phenobarbital and its potentiation by dibutyryl cAMP assayed in normal rat hepatocytes are associated with increased activity of ferrochelatase mRNA. Glucose inhibits this stimulatory effect. This inhibition can be reversed with increasing concentrations o...

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Autores principales: Cánepa, E.T., Pereda, M.P., Llambías, E.B., Grinstein, M.
Formato: JOUR
Materias:
bucladesine
ferrochelatase
glucose
messenger RNA
phenobarbital
animal
article
biosynthesis
drug effect
enzyme induction
experimental diabetes mellitus
gene expression regulation
liver
male
metabolism
rat
rat strain
Animals
Bucladesine
Diabetes Mellitus, Experimental
Enzyme Induction
Ferrochelatase
Gene Expression Regulation
Glucose
Liver
Male
Phenobarbital
Rats
Rats, Inbred Strains
RNA, Messenger
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_08298211_v70_n1_p26_Canepa
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_08298211_v70_n1_p26_Canepa
record_format dspace
spelling todo:paper_08298211_v70_n1_p26_Canepa2023-10-03T15:40:10Z Regulation of phenobarbital-induced ferrochelatase mRNA activity by dibutyryl cAMP and glucose in normal and diabetic rat hepatocytes. Cánepa, E.T. Pereda, M.P. Llambías, E.B. Grinstein, M. bucladesine ferrochelatase glucose messenger RNA phenobarbital animal article biosynthesis drug effect enzyme induction experimental diabetes mellitus gene expression regulation liver male metabolism rat rat strain Animals Bucladesine Diabetes Mellitus, Experimental Enzyme Induction Ferrochelatase Gene Expression Regulation Glucose Liver Male Phenobarbital Rats Rats, Inbred Strains RNA, Messenger The induction of ferrochelatase activity by phenobarbital and its potentiation by dibutyryl cAMP assayed in normal rat hepatocytes are associated with increased activity of ferrochelatase mRNA. Glucose inhibits this stimulatory effect. This inhibition can be reversed with increasing concentrations of dibutyryl cAMP. The inducing effect exerted by phenobarbital on the activity of ferrochelatase mRNA in diabetic hepatocytes is greater than that observed in normal cells. This enhanced response in diabetic rat hepatocytes is neither potentiated by adding dibutyryl cAMP nor repressed by glucose. The absence of a glucose effect persists even when the endogenous cAMP content is lowered to normal levels. The results obtained in this study are consistent with those reported in other published studies of ferrochelatase activity. This adds more experimental evidence to support the concept that ferrochelatase is inducible. The results obtained suggest that ferrochelatase is more susceptible to induction with phenobarbital in diabetic rat hepatocytes than in normal rat hepatocytes. Fil:Cánepa, E.T. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Pereda, M.P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Llambías, E.B. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Grinstein, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_08298211_v70_n1_p26_Canepa
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic bucladesine
ferrochelatase
glucose
messenger RNA
phenobarbital
animal
article
biosynthesis
drug effect
enzyme induction
experimental diabetes mellitus
gene expression regulation
liver
male
metabolism
rat
rat strain
Animals
Bucladesine
Diabetes Mellitus, Experimental
Enzyme Induction
Ferrochelatase
Gene Expression Regulation
Glucose
Liver
Male
Phenobarbital
Rats
Rats, Inbred Strains
RNA, Messenger
spellingShingle bucladesine
ferrochelatase
glucose
messenger RNA
phenobarbital
animal
article
biosynthesis
drug effect
enzyme induction
experimental diabetes mellitus
gene expression regulation
liver
male
metabolism
rat
rat strain
Animals
Bucladesine
Diabetes Mellitus, Experimental
Enzyme Induction
Ferrochelatase
Gene Expression Regulation
Glucose
Liver
Male
Phenobarbital
Rats
Rats, Inbred Strains
RNA, Messenger
Cánepa, E.T.
Pereda, M.P.
Llambías, E.B.
Grinstein, M.
Regulation of phenobarbital-induced ferrochelatase mRNA activity by dibutyryl cAMP and glucose in normal and diabetic rat hepatocytes.
topic_facet bucladesine
ferrochelatase
glucose
messenger RNA
phenobarbital
animal
article
biosynthesis
drug effect
enzyme induction
experimental diabetes mellitus
gene expression regulation
liver
male
metabolism
rat
rat strain
Animals
Bucladesine
Diabetes Mellitus, Experimental
Enzyme Induction
Ferrochelatase
Gene Expression Regulation
Glucose
Liver
Male
Phenobarbital
Rats
Rats, Inbred Strains
RNA, Messenger
description The induction of ferrochelatase activity by phenobarbital and its potentiation by dibutyryl cAMP assayed in normal rat hepatocytes are associated with increased activity of ferrochelatase mRNA. Glucose inhibits this stimulatory effect. This inhibition can be reversed with increasing concentrations of dibutyryl cAMP. The inducing effect exerted by phenobarbital on the activity of ferrochelatase mRNA in diabetic hepatocytes is greater than that observed in normal cells. This enhanced response in diabetic rat hepatocytes is neither potentiated by adding dibutyryl cAMP nor repressed by glucose. The absence of a glucose effect persists even when the endogenous cAMP content is lowered to normal levels. The results obtained in this study are consistent with those reported in other published studies of ferrochelatase activity. This adds more experimental evidence to support the concept that ferrochelatase is inducible. The results obtained suggest that ferrochelatase is more susceptible to induction with phenobarbital in diabetic rat hepatocytes than in normal rat hepatocytes.
format JOUR
author Cánepa, E.T.
Pereda, M.P.
Llambías, E.B.
Grinstein, M.
author_facet Cánepa, E.T.
Pereda, M.P.
Llambías, E.B.
Grinstein, M.
author_sort Cánepa, E.T.
title Regulation of phenobarbital-induced ferrochelatase mRNA activity by dibutyryl cAMP and glucose in normal and diabetic rat hepatocytes.
title_short Regulation of phenobarbital-induced ferrochelatase mRNA activity by dibutyryl cAMP and glucose in normal and diabetic rat hepatocytes.
title_full Regulation of phenobarbital-induced ferrochelatase mRNA activity by dibutyryl cAMP and glucose in normal and diabetic rat hepatocytes.
title_fullStr Regulation of phenobarbital-induced ferrochelatase mRNA activity by dibutyryl cAMP and glucose in normal and diabetic rat hepatocytes.
title_full_unstemmed Regulation of phenobarbital-induced ferrochelatase mRNA activity by dibutyryl cAMP and glucose in normal and diabetic rat hepatocytes.
title_sort regulation of phenobarbital-induced ferrochelatase mrna activity by dibutyryl camp and glucose in normal and diabetic rat hepatocytes.
url http://hdl.handle.net/20.500.12110/paper_08298211_v70_n1_p26_Canepa
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AT llambiaseb regulationofphenobarbitalinducedferrochelatasemrnaactivitybydibutyrylcampandglucoseinnormalanddiabeticrathepatocytes
AT grinsteinm regulationofphenobarbitalinducedferrochelatasemrnaactivitybydibutyrylcampandglucoseinnormalanddiabeticrathepatocytes
_version_ 1807319450008944640

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