Selection strategy for a seedling seed orchard design based on trait selection index and genomic analysis by molecular markers: A case study for Eucalyptus dunnii

Molecular genetic analysis was applied to 162 individuals of 37 half-sib selected families belonging to six provenances of Eucalyptus dunnii Maiden in a provenance/family trial. The individuals were selected by a trait selection index and genetic diversity criteria were later applied for designing s...

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Autores principales: Zelener, N., Marcucci Poltri, S.N., Bartoloni, N., López, C.R., Hopp, H.E.
Formato: JOUR
Materias:
AFLP
AMOVA
DNA fingerprinting
Forest tree improvement
Genetic diversity
Genetic relationship
Microsatellites
Population genetic structure
Simple sequence repeats (SSRs)
genetic variation
plant breeding
provenance trial
silviculture
Eucalyptus
Eucalyptus dunnii
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_0829318X_v25_n11_p1457_Zelener
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_0829318X_v25_n11_p1457_Zelener
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spelling todo:paper_0829318X_v25_n11_p1457_Zelener2023-10-03T15:40:02Z Selection strategy for a seedling seed orchard design based on trait selection index and genomic analysis by molecular markers: A case study for Eucalyptus dunnii Zelener, N. Marcucci Poltri, S.N. Bartoloni, N. López, C.R. Hopp, H.E. AFLP AMOVA DNA fingerprinting Forest tree improvement Genetic diversity Genetic relationship Microsatellites Population genetic structure Simple sequence repeats (SSRs) DNA fingerprinting genetic variation plant breeding provenance trial silviculture Eucalyptus Eucalyptus dunnii Molecular genetic analysis was applied to 162 individuals of 37 half-sib selected families belonging to six provenances of Eucalyptus dunnii Maiden in a provenance/family trial. The individuals were selected by a trait selection index and genetic diversity criteria were later applied for designing seedling seed orchards. Genetic diversity and its distribution, as well as relationships among individuals, were assessed on the basis of nine microsatellite loci and 243 amplified fragment length polymorphism markers. High diversity was found with both kinds of markers. Clear-cut genomic patterns of identification (fingerprinting) were obtained for each individual. Genetic differentiation estimates consistently showed low differentiation among provenances (R ST1 = 0.069, θP = 0.026 and FCT = 0.035) and great differentiation among families (RST2 = 0.223, θS = 0.174 and FSC = 0.164). A high proportion of the total variation was observed within families (around 80% by both marker analyses), suggesting that orchard design should be based on individual or family selection rather than on provenance selection, and that individual ranking by both trait selection index and molecular genetic diversity criteria should be considered. A selection procedure for a seedling seed orchard design is proposed. © 2005 Heron Publishing. Fil:Marcucci Poltri, S.N. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Hopp, H.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_0829318X_v25_n11_p1457_Zelener
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic AFLP
AMOVA
DNA fingerprinting
Forest tree improvement
Genetic diversity
Genetic relationship
Microsatellites
Population genetic structure
Simple sequence repeats (SSRs)
DNA fingerprinting
genetic variation
plant breeding
provenance trial
silviculture
Eucalyptus
Eucalyptus dunnii
spellingShingle AFLP
AMOVA
DNA fingerprinting
Forest tree improvement
Genetic diversity
Genetic relationship
Microsatellites
Population genetic structure
Simple sequence repeats (SSRs)
DNA fingerprinting
genetic variation
plant breeding
provenance trial
silviculture
Eucalyptus
Eucalyptus dunnii
Zelener, N.
Marcucci Poltri, S.N.
Bartoloni, N.
López, C.R.
Hopp, H.E.
Selection strategy for a seedling seed orchard design based on trait selection index and genomic analysis by molecular markers: A case study for Eucalyptus dunnii
topic_facet AFLP
AMOVA
DNA fingerprinting
Forest tree improvement
Genetic diversity
Genetic relationship
Microsatellites
Population genetic structure
Simple sequence repeats (SSRs)
DNA fingerprinting
genetic variation
plant breeding
provenance trial
silviculture
Eucalyptus
Eucalyptus dunnii
description Molecular genetic analysis was applied to 162 individuals of 37 half-sib selected families belonging to six provenances of Eucalyptus dunnii Maiden in a provenance/family trial. The individuals were selected by a trait selection index and genetic diversity criteria were later applied for designing seedling seed orchards. Genetic diversity and its distribution, as well as relationships among individuals, were assessed on the basis of nine microsatellite loci and 243 amplified fragment length polymorphism markers. High diversity was found with both kinds of markers. Clear-cut genomic patterns of identification (fingerprinting) were obtained for each individual. Genetic differentiation estimates consistently showed low differentiation among provenances (R ST1 = 0.069, θP = 0.026 and FCT = 0.035) and great differentiation among families (RST2 = 0.223, θS = 0.174 and FSC = 0.164). A high proportion of the total variation was observed within families (around 80% by both marker analyses), suggesting that orchard design should be based on individual or family selection rather than on provenance selection, and that individual ranking by both trait selection index and molecular genetic diversity criteria should be considered. A selection procedure for a seedling seed orchard design is proposed. © 2005 Heron Publishing.
format JOUR
author Zelener, N.
Marcucci Poltri, S.N.
Bartoloni, N.
López, C.R.
Hopp, H.E.
author_facet Zelener, N.
Marcucci Poltri, S.N.
Bartoloni, N.
López, C.R.
Hopp, H.E.
author_sort Zelener, N.
title Selection strategy for a seedling seed orchard design based on trait selection index and genomic analysis by molecular markers: A case study for Eucalyptus dunnii
title_short Selection strategy for a seedling seed orchard design based on trait selection index and genomic analysis by molecular markers: A case study for Eucalyptus dunnii
title_full Selection strategy for a seedling seed orchard design based on trait selection index and genomic analysis by molecular markers: A case study for Eucalyptus dunnii
title_fullStr Selection strategy for a seedling seed orchard design based on trait selection index and genomic analysis by molecular markers: A case study for Eucalyptus dunnii
title_full_unstemmed Selection strategy for a seedling seed orchard design based on trait selection index and genomic analysis by molecular markers: A case study for Eucalyptus dunnii
title_sort selection strategy for a seedling seed orchard design based on trait selection index and genomic analysis by molecular markers: a case study for eucalyptus dunnii
url http://hdl.handle.net/20.500.12110/paper_0829318X_v25_n11_p1457_Zelener
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