Lymphocyte subsets and cytokines in adenoviral infection in children
To determine the distribution of major blood lymphocyte subsets we evaluated blood lymphocytes by flow cytometry in adenovirus-infected infants aged 30-730 d. In addition, interleukin-1-receptor antagonist, interleukin- 10 and transforming growth factor-β1 were measured in serum by enzyme- linked im...
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_08035253_v87_n9_p933_Mistchenko |
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todo:paper_08035253_v87_n9_p933_Mistchenko2023-10-03T15:39:56Z Lymphocyte subsets and cytokines in adenoviral infection in children Mistchenko, A.S. Koch, E.R.R. Kajon, A.E. Tibaldi, F. Maffey, A.F. Diez, R.A. Adenovirus Cytokines Lymphocytes Respiratory infection cd16 antigen cd19 antigen cd4 antigen cd5 antigen cd8 antigen cytokine interleukin 1 receptor blocking agent interleukin 10 transforming growth factor beta1 adenovirus article artificial ventilation clinical article controlled study female helper cell human infant inflammation male natural killer cell preschool child priority journal respiratory tract infection t lymphocyte t lymphocyte subpopulation virus infection Adenoviridae Infections Child, Preschool Cytokines Female Humans Infant Lymphocyte Subsets Male Phenotype To determine the distribution of major blood lymphocyte subsets we evaluated blood lymphocytes by flow cytometry in adenovirus-infected infants aged 30-730 d. In addition, interleukin-1-receptor antagonist, interleukin- 10 and transforming growth factor-β1 were measured in serum by enzyme- linked immunosorbent assay. According to clinical parameters, mechanical ventilation and outcome, infections were classified as moderate (n = 15), severe (n = 11) and fatal (n = 12). Controls were 13 healthy children. In severe and fatal infection, T cells (CD5+/CD19-), NK effectors (CD16+), CD4+ T subset and B1 subset of B lymphocytes (CD5+/CD19+) were all significantly decreased. CD8+ cells were decreased in severe but not fatal cases. There was no difference in serum values of interleukin-10; however, fatal cases had high interleukin 1-receptor antagonist values. Interestingly, patients with moderate infection showed significantly increased values of transforming growth factor-β1. These results demonstrate that life- threatening adenoviral infection is associated with marked abnormalities in blood lymphocyte and cytokine profile. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_08035253_v87_n9_p933_Mistchenko |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Adenovirus Cytokines Lymphocytes Respiratory infection cd16 antigen cd19 antigen cd4 antigen cd5 antigen cd8 antigen cytokine interleukin 1 receptor blocking agent interleukin 10 transforming growth factor beta1 adenovirus article artificial ventilation clinical article controlled study female helper cell human infant inflammation male natural killer cell preschool child priority journal respiratory tract infection t lymphocyte t lymphocyte subpopulation virus infection Adenoviridae Infections Child, Preschool Cytokines Female Humans Infant Lymphocyte Subsets Male Phenotype |
| spellingShingle |
Adenovirus Cytokines Lymphocytes Respiratory infection cd16 antigen cd19 antigen cd4 antigen cd5 antigen cd8 antigen cytokine interleukin 1 receptor blocking agent interleukin 10 transforming growth factor beta1 adenovirus article artificial ventilation clinical article controlled study female helper cell human infant inflammation male natural killer cell preschool child priority journal respiratory tract infection t lymphocyte t lymphocyte subpopulation virus infection Adenoviridae Infections Child, Preschool Cytokines Female Humans Infant Lymphocyte Subsets Male Phenotype Mistchenko, A.S. Koch, E.R.R. Kajon, A.E. Tibaldi, F. Maffey, A.F. Diez, R.A. Lymphocyte subsets and cytokines in adenoviral infection in children |
| topic_facet |
Adenovirus Cytokines Lymphocytes Respiratory infection cd16 antigen cd19 antigen cd4 antigen cd5 antigen cd8 antigen cytokine interleukin 1 receptor blocking agent interleukin 10 transforming growth factor beta1 adenovirus article artificial ventilation clinical article controlled study female helper cell human infant inflammation male natural killer cell preschool child priority journal respiratory tract infection t lymphocyte t lymphocyte subpopulation virus infection Adenoviridae Infections Child, Preschool Cytokines Female Humans Infant Lymphocyte Subsets Male Phenotype |
| description |
To determine the distribution of major blood lymphocyte subsets we evaluated blood lymphocytes by flow cytometry in adenovirus-infected infants aged 30-730 d. In addition, interleukin-1-receptor antagonist, interleukin- 10 and transforming growth factor-β1 were measured in serum by enzyme- linked immunosorbent assay. According to clinical parameters, mechanical ventilation and outcome, infections were classified as moderate (n = 15), severe (n = 11) and fatal (n = 12). Controls were 13 healthy children. In severe and fatal infection, T cells (CD5+/CD19-), NK effectors (CD16+), CD4+ T subset and B1 subset of B lymphocytes (CD5+/CD19+) were all significantly decreased. CD8+ cells were decreased in severe but not fatal cases. There was no difference in serum values of interleukin-10; however, fatal cases had high interleukin 1-receptor antagonist values. Interestingly, patients with moderate infection showed significantly increased values of transforming growth factor-β1. These results demonstrate that life- threatening adenoviral infection is associated with marked abnormalities in blood lymphocyte and cytokine profile. |
| format |
JOUR |
| author |
Mistchenko, A.S. Koch, E.R.R. Kajon, A.E. Tibaldi, F. Maffey, A.F. Diez, R.A. |
| author_facet |
Mistchenko, A.S. Koch, E.R.R. Kajon, A.E. Tibaldi, F. Maffey, A.F. Diez, R.A. |
| author_sort |
Mistchenko, A.S. |
| title |
Lymphocyte subsets and cytokines in adenoviral infection in children |
| title_short |
Lymphocyte subsets and cytokines in adenoviral infection in children |
| title_full |
Lymphocyte subsets and cytokines in adenoviral infection in children |
| title_fullStr |
Lymphocyte subsets and cytokines in adenoviral infection in children |
| title_full_unstemmed |
Lymphocyte subsets and cytokines in adenoviral infection in children |
| title_sort |
lymphocyte subsets and cytokines in adenoviral infection in children |
| url |
http://hdl.handle.net/20.500.12110/paper_08035253_v87_n9_p933_Mistchenko |
| work_keys_str_mv |
AT mistchenkoas lymphocytesubsetsandcytokinesinadenoviralinfectioninchildren AT kocherr lymphocytesubsetsandcytokinesinadenoviralinfectioninchildren AT kajonae lymphocytesubsetsandcytokinesinadenoviralinfectioninchildren AT tibaldif lymphocytesubsetsandcytokinesinadenoviralinfectioninchildren AT maffeyaf lymphocytesubsetsandcytokinesinadenoviralinfectioninchildren AT diezra lymphocytesubsetsandcytokinesinadenoviralinfectioninchildren |
| _version_ |
1807317064731328512 |