In vitro effect of methyl farnesoate on the vitellogenin content of ovary and hepatopancreas, in the crayfish Cherax quadricarinatus

Vitellogenin levels were determined in pieces of either ovary or hepatopancreas taken from females of the crayfish Cherax quadricarinatus during both early pre-reproductive and reproductive periods, and exposed in vitro to 0.15, 1.5, or 15 μM of the juvenoid methyl farnesoate (MF). A significant acc...

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Autores principales: Medesani, D.A., Ferré, L.E., Grodzielski, M., Rodríguez, E.M.
Formato: JOUR
Materias:
Crustaceans
Induction
Juvenoids
Reproduction
Astacoidea
Cherax quadricarinatus
Crustacea
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_07924259_v56_n2_p138_Medesani
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
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spelling todo:paper_07924259_v56_n2_p138_Medesani2023-10-03T15:39:54Z In vitro effect of methyl farnesoate on the vitellogenin content of ovary and hepatopancreas, in the crayfish Cherax quadricarinatus Medesani, D.A. Ferré, L.E. Grodzielski, M. Rodríguez, E.M. Crustaceans Induction Juvenoids Reproduction Astacoidea Cherax quadricarinatus Crustacea Vitellogenin levels were determined in pieces of either ovary or hepatopancreas taken from females of the crayfish Cherax quadricarinatus during both early pre-reproductive and reproductive periods, and exposed in vitro to 0.15, 1.5, or 15 μM of the juvenoid methyl farnesoate (MF). A significant accumulation of vitellogenin was seen in ovaries treated with 15 μM of MF during the early pre-reproductive period. Besides, protein synthesis, measured as tritiated leucine incorporation, was correspondingly increased in ovaries taken during both pre- and post-reproductive periods and exposed to 15 μM of MF. On the other hand, no stimulating effect of MF on the vitellogenin content of the hepatopancreas was seen in any period. These results, taken together, support the hypothesis of the endogenous vitellogenin synthesis stimulated by MF during the early ovarian maturation. However, this hormone was not able to stimulate vitellogenin synthesis in the ovary during the reproductive period. © 2012 Taylor & Francis. Fil:Medesani, D.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Ferré, L.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Rodríguez, E.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_07924259_v56_n2_p138_Medesani
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Crustaceans
Induction
Juvenoids
Reproduction
Astacoidea
Cherax quadricarinatus
Crustacea
spellingShingle Crustaceans
Induction
Juvenoids
Reproduction
Astacoidea
Cherax quadricarinatus
Crustacea
Medesani, D.A.
Ferré, L.E.
Grodzielski, M.
Rodríguez, E.M.
In vitro effect of methyl farnesoate on the vitellogenin content of ovary and hepatopancreas, in the crayfish Cherax quadricarinatus
topic_facet Crustaceans
Induction
Juvenoids
Reproduction
Astacoidea
Cherax quadricarinatus
Crustacea
description Vitellogenin levels were determined in pieces of either ovary or hepatopancreas taken from females of the crayfish Cherax quadricarinatus during both early pre-reproductive and reproductive periods, and exposed in vitro to 0.15, 1.5, or 15 μM of the juvenoid methyl farnesoate (MF). A significant accumulation of vitellogenin was seen in ovaries treated with 15 μM of MF during the early pre-reproductive period. Besides, protein synthesis, measured as tritiated leucine incorporation, was correspondingly increased in ovaries taken during both pre- and post-reproductive periods and exposed to 15 μM of MF. On the other hand, no stimulating effect of MF on the vitellogenin content of the hepatopancreas was seen in any period. These results, taken together, support the hypothesis of the endogenous vitellogenin synthesis stimulated by MF during the early ovarian maturation. However, this hormone was not able to stimulate vitellogenin synthesis in the ovary during the reproductive period. © 2012 Taylor & Francis.
format JOUR
author Medesani, D.A.
Ferré, L.E.
Grodzielski, M.
Rodríguez, E.M.
author_facet Medesani, D.A.
Ferré, L.E.
Grodzielski, M.
Rodríguez, E.M.
author_sort Medesani, D.A.
title In vitro effect of methyl farnesoate on the vitellogenin content of ovary and hepatopancreas, in the crayfish Cherax quadricarinatus
title_short In vitro effect of methyl farnesoate on the vitellogenin content of ovary and hepatopancreas, in the crayfish Cherax quadricarinatus
title_full In vitro effect of methyl farnesoate on the vitellogenin content of ovary and hepatopancreas, in the crayfish Cherax quadricarinatus
title_fullStr In vitro effect of methyl farnesoate on the vitellogenin content of ovary and hepatopancreas, in the crayfish Cherax quadricarinatus
title_full_unstemmed In vitro effect of methyl farnesoate on the vitellogenin content of ovary and hepatopancreas, in the crayfish Cherax quadricarinatus
title_sort in vitro effect of methyl farnesoate on the vitellogenin content of ovary and hepatopancreas, in the crayfish cherax quadricarinatus
url http://hdl.handle.net/20.500.12110/paper_07924259_v56_n2_p138_Medesani
work_keys_str_mv AT medesanida invitroeffectofmethylfarnesoateonthevitellogenincontentofovaryandhepatopancreasinthecrayfishcheraxquadricarinatus
AT ferrele invitroeffectofmethylfarnesoateonthevitellogenincontentofovaryandhepatopancreasinthecrayfishcheraxquadricarinatus
AT grodzielskim invitroeffectofmethylfarnesoateonthevitellogenincontentofovaryandhepatopancreasinthecrayfishcheraxquadricarinatus
AT rodriguezem invitroeffectofmethylfarnesoateonthevitellogenincontentofovaryandhepatopancreasinthecrayfishcheraxquadricarinatus
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