Redox-active concanavalin a: Synthesis, characterization, and recognition-driven assembly of interfacial architectures for bioelectronic applications
The convergence of chemistry, biology, and materials science has paved the way to the emergence of hybrid nanobuilding blocks that incorporate the highly selective recognition properties of biomolecules, with the tailorable functional capabilities of inorganic molecules. In this work, we describe fo...
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todo:paper_07437463_v26_n16_p13684_Pallarola2023-10-03T15:38:41Z Redox-active concanavalin a: Synthesis, characterization, and recognition-driven assembly of interfacial architectures for bioelectronic applications Pallarola, D. Queralto, N. Knoll, W. Ceoli Azzaroni, O. Battaglini, F. Bio-molecular Bioelectrochemical systems Bioelectronic applications Building blockes Concanavalin A Electroactive Functional capabilities Functional features Glycoconjugates Gold electrodes Inorganic complexes Inorganic molecules Interfacial architecture MALDI TOF MS Matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry Metal electrodes Molecular modification Multi-layered Nanobuilding blocks Recognition process Recognition properties Redox activity Redox potentials Redox-active Selective recognition Small angle X-ray scattering Spontaneous formation Steady-state fluorescence Supramolecular assemblies Surface plasmon resonance spectroscopy Biosensors Carbohydrates Characterization Complexation Cyclic voltammetry Desorption Electrodes Functional polymers Glucose Glucose oxidase Glucose sensors Hybrid materials Mass spectrometry Molecular biology Osmium Pulsed laser applications Pulsed lasers Redox reactions Sugar (sucrose) Surface plasmon resonance X ray scattering Building materials concanavalin A nanomaterial article chemistry electrochemistry mass spectrometry small angle scattering surface plasmon resonance theoretical model Concanavalin A Electrochemistry Models, Theoretical Nanostructures Scattering, Small Angle Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Surface Plasmon Resonance The convergence of chemistry, biology, and materials science has paved the way to the emergence of hybrid nanobuilding blocks that incorporate the highly selective recognition properties of biomolecules, with the tailorable functional capabilities of inorganic molecules. In this work, we describe for the first time the decoration of concanavalin A (Con A), a protein with the ability to recognize sugars and form glycoconjugates, with Os(II) redox-active complexes. This strategy enabled the construction of electroactive biosupramolecular materials whose redox potentials could be easily modulated through the facile molecular modification of the electroactive inorganic complexes. Small-angle X-ray scattering (SAXS), steady-state fluorescence, surface plasmon resonance (SPR) spectroscopy, matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-TOF-MS), and differential-pulsed (DPV) and cyclic voltammetry (CV) were used to characterize the structural and functional features of the synthesized biohybrid building blocks as well as their respective supramolecular assemblies built up on gold electrodes. By harnessing the electroactive and carbohydrate-recognition properties of these tailor-made biohybrid building blocks, we were able to integrate glucose oxidase (GOx) onto gold electrodes via sugar'lectin interactions. The redox activity of the Os-modified Con A interlayer allowed the electronic connection between the multilayered GOx assemblies and the metal electrode as evidenced by the well-defined bioelectrocatalytic response exhibited by the biomolecular assemblies in the presence of the glucose in solution. We consider that this approach based on the spontaneous formation of redox-active biosupramolecular assemblies driven by recognition processes can be of practical relevance for the facile design of biosensors, as well as for the construction of new multifunctional bioelectrochemical systems. © 2010 American Chemical Society. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_07437463_v26_n16_p13684_Pallarola |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Bio-molecular Bioelectrochemical systems Bioelectronic applications Building blockes Concanavalin A Electroactive Functional capabilities Functional features Glycoconjugates Gold electrodes Inorganic complexes Inorganic molecules Interfacial architecture MALDI TOF MS Matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry Metal electrodes Molecular modification Multi-layered Nanobuilding blocks Recognition process Recognition properties Redox activity Redox potentials Redox-active Selective recognition Small angle X-ray scattering Spontaneous formation Steady-state fluorescence Supramolecular assemblies Surface plasmon resonance spectroscopy Biosensors Carbohydrates Characterization Complexation Cyclic voltammetry Desorption Electrodes Functional polymers Glucose Glucose oxidase Glucose sensors Hybrid materials Mass spectrometry Molecular biology Osmium Pulsed laser applications Pulsed lasers Redox reactions Sugar (sucrose) Surface plasmon resonance X ray scattering Building materials concanavalin A nanomaterial article chemistry electrochemistry mass spectrometry small angle scattering surface plasmon resonance theoretical model Concanavalin A Electrochemistry Models, Theoretical Nanostructures Scattering, Small Angle Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Surface Plasmon Resonance |
| spellingShingle |
Bio-molecular Bioelectrochemical systems Bioelectronic applications Building blockes Concanavalin A Electroactive Functional capabilities Functional features Glycoconjugates Gold electrodes Inorganic complexes Inorganic molecules Interfacial architecture MALDI TOF MS Matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry Metal electrodes Molecular modification Multi-layered Nanobuilding blocks Recognition process Recognition properties Redox activity Redox potentials Redox-active Selective recognition Small angle X-ray scattering Spontaneous formation Steady-state fluorescence Supramolecular assemblies Surface plasmon resonance spectroscopy Biosensors Carbohydrates Characterization Complexation Cyclic voltammetry Desorption Electrodes Functional polymers Glucose Glucose oxidase Glucose sensors Hybrid materials Mass spectrometry Molecular biology Osmium Pulsed laser applications Pulsed lasers Redox reactions Sugar (sucrose) Surface plasmon resonance X ray scattering Building materials concanavalin A nanomaterial article chemistry electrochemistry mass spectrometry small angle scattering surface plasmon resonance theoretical model Concanavalin A Electrochemistry Models, Theoretical Nanostructures Scattering, Small Angle Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Surface Plasmon Resonance Pallarola, D. Queralto, N. Knoll, W. Ceoli Azzaroni, O. Battaglini, F. Redox-active concanavalin a: Synthesis, characterization, and recognition-driven assembly of interfacial architectures for bioelectronic applications |
| topic_facet |
Bio-molecular Bioelectrochemical systems Bioelectronic applications Building blockes Concanavalin A Electroactive Functional capabilities Functional features Glycoconjugates Gold electrodes Inorganic complexes Inorganic molecules Interfacial architecture MALDI TOF MS Matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry Metal electrodes Molecular modification Multi-layered Nanobuilding blocks Recognition process Recognition properties Redox activity Redox potentials Redox-active Selective recognition Small angle X-ray scattering Spontaneous formation Steady-state fluorescence Supramolecular assemblies Surface plasmon resonance spectroscopy Biosensors Carbohydrates Characterization Complexation Cyclic voltammetry Desorption Electrodes Functional polymers Glucose Glucose oxidase Glucose sensors Hybrid materials Mass spectrometry Molecular biology Osmium Pulsed laser applications Pulsed lasers Redox reactions Sugar (sucrose) Surface plasmon resonance X ray scattering Building materials concanavalin A nanomaterial article chemistry electrochemistry mass spectrometry small angle scattering surface plasmon resonance theoretical model Concanavalin A Electrochemistry Models, Theoretical Nanostructures Scattering, Small Angle Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Surface Plasmon Resonance |
| description |
The convergence of chemistry, biology, and materials science has paved the way to the emergence of hybrid nanobuilding blocks that incorporate the highly selective recognition properties of biomolecules, with the tailorable functional capabilities of inorganic molecules. In this work, we describe for the first time the decoration of concanavalin A (Con A), a protein with the ability to recognize sugars and form glycoconjugates, with Os(II) redox-active complexes. This strategy enabled the construction of electroactive biosupramolecular materials whose redox potentials could be easily modulated through the facile molecular modification of the electroactive inorganic complexes. Small-angle X-ray scattering (SAXS), steady-state fluorescence, surface plasmon resonance (SPR) spectroscopy, matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-TOF-MS), and differential-pulsed (DPV) and cyclic voltammetry (CV) were used to characterize the structural and functional features of the synthesized biohybrid building blocks as well as their respective supramolecular assemblies built up on gold electrodes. By harnessing the electroactive and carbohydrate-recognition properties of these tailor-made biohybrid building blocks, we were able to integrate glucose oxidase (GOx) onto gold electrodes via sugar'lectin interactions. The redox activity of the Os-modified Con A interlayer allowed the electronic connection between the multilayered GOx assemblies and the metal electrode as evidenced by the well-defined bioelectrocatalytic response exhibited by the biomolecular assemblies in the presence of the glucose in solution. We consider that this approach based on the spontaneous formation of redox-active biosupramolecular assemblies driven by recognition processes can be of practical relevance for the facile design of biosensors, as well as for the construction of new multifunctional bioelectrochemical systems. © 2010 American Chemical Society. |
| format |
JOUR |
| author |
Pallarola, D. Queralto, N. Knoll, W. Ceoli Azzaroni, O. Battaglini, F. |
| author_facet |
Pallarola, D. Queralto, N. Knoll, W. Ceoli Azzaroni, O. Battaglini, F. |
| author_sort |
Pallarola, D. |
| title |
Redox-active concanavalin a: Synthesis, characterization, and recognition-driven assembly of interfacial architectures for bioelectronic applications |
| title_short |
Redox-active concanavalin a: Synthesis, characterization, and recognition-driven assembly of interfacial architectures for bioelectronic applications |
| title_full |
Redox-active concanavalin a: Synthesis, characterization, and recognition-driven assembly of interfacial architectures for bioelectronic applications |
| title_fullStr |
Redox-active concanavalin a: Synthesis, characterization, and recognition-driven assembly of interfacial architectures for bioelectronic applications |
| title_full_unstemmed |
Redox-active concanavalin a: Synthesis, characterization, and recognition-driven assembly of interfacial architectures for bioelectronic applications |
| title_sort |
redox-active concanavalin a: synthesis, characterization, and recognition-driven assembly of interfacial architectures for bioelectronic applications |
| url |
http://hdl.handle.net/20.500.12110/paper_07437463_v26_n16_p13684_Pallarola |
| work_keys_str_mv |
AT pallarolad redoxactiveconcanavalinasynthesischaracterizationandrecognitiondrivenassemblyofinterfacialarchitecturesforbioelectronicapplications AT queralton redoxactiveconcanavalinasynthesischaracterizationandrecognitiondrivenassemblyofinterfacialarchitecturesforbioelectronicapplications AT knollw redoxactiveconcanavalinasynthesischaracterizationandrecognitiondrivenassemblyofinterfacialarchitecturesforbioelectronicapplications AT ceoli redoxactiveconcanavalinasynthesischaracterizationandrecognitiondrivenassemblyofinterfacialarchitecturesforbioelectronicapplications AT azzaronio redoxactiveconcanavalinasynthesischaracterizationandrecognitiondrivenassemblyofinterfacialarchitecturesforbioelectronicapplications AT battaglinif redoxactiveconcanavalinasynthesischaracterizationandrecognitiondrivenassemblyofinterfacialarchitecturesforbioelectronicapplications |
| _version_ |
1782025643158929408 |