Quantitative electrochemical SERS of flavin at a structured silver surface

In situ electrochemical surface enhanced Raman spectra (SERS) for an immobilized monolayer of a flavin analogue (isoalloxazine) at nanostructured silver surfaces are reported. Unique in the present study, the flavin is not directly adsorbed at the Ag surface but is attached through a chemical reacti...

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Autores principales: Abdelsalam, M., Bartlett, P.N., Russell, A.E., Baumberg, J.J., Calvo, E.J., Tognalli, N.G., Fainstein, A.
Formato: JOUR
Materias:
Ag(111) surface
Colloidal templates
Cysteamine
Direct contacts
Electrochemical SERS
Electrochemical surfaces
In-situ
Nano-structured
Silver surface
Surface enhancements
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_07437463_v24_n13_p7018_Abdelsalam
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_07437463_v24_n13_p7018_Abdelsalam
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spelling todo:paper_07437463_v24_n13_p7018_Abdelsalam2023-10-03T15:38:38Z Quantitative electrochemical SERS of flavin at a structured silver surface Abdelsalam, M. Bartlett, P.N. Russell, A.E. Baumberg, J.J. Calvo, E.J. Tognalli, N.G. Fainstein, A. Ag(111) surface Colloidal templates Cysteamine Direct contacts Electrochemical SERS Electrochemical surfaces In-situ Nano-structured Silver surface Surface enhancements In situ electrochemical surface enhanced Raman spectra (SERS) for an immobilized monolayer of a flavin analogue (isoalloxazine) at nanostructured silver surfaces are reported. Unique in the present study, the flavin is not directly adsorbed at the Ag surface but is attached through a chemical reaction between cysteamine adsorbed on the Ag surface and methylformylisoalloxazine. Even though the flavin is held away from direct contact with the metal, strong surface enhancements are observed. The nanostructured silver surfaces are produced by electrodeposition through colloidal templates to produce thin (< 1 μm) films containing close-packed hexagonal arrays of uniform 900 nm sphere segment voids. The sphere segment void (SSV) structured silver surfaces are shown to be ideally suited to in situ electrochemical SERS studies at 633 nm, giving stable, reproducible surface enhancements at a range of electrode potentials, and we show that the SER spectra are sensitive to subfemtomole quantities of immobilized flavin. Studies of the SER spectra as a function of the electrode potential show clear evidence for the formation of the flavin semiquinone at the electrode surface at cathodic potentials. © 2008 American Chemical Society. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_07437463_v24_n13_p7018_Abdelsalam
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Ag(111) surface
Colloidal templates
Cysteamine
Direct contacts
Electrochemical SERS
Electrochemical surfaces
In-situ
Nano-structured
Silver surface
Surface enhancements
spellingShingle Ag(111) surface
Colloidal templates
Cysteamine
Direct contacts
Electrochemical SERS
Electrochemical surfaces
In-situ
Nano-structured
Silver surface
Surface enhancements
Abdelsalam, M.
Bartlett, P.N.
Russell, A.E.
Baumberg, J.J.
Calvo, E.J.
Tognalli, N.G.
Fainstein, A.
Quantitative electrochemical SERS of flavin at a structured silver surface
topic_facet Ag(111) surface
Colloidal templates
Cysteamine
Direct contacts
Electrochemical SERS
Electrochemical surfaces
In-situ
Nano-structured
Silver surface
Surface enhancements
description In situ electrochemical surface enhanced Raman spectra (SERS) for an immobilized monolayer of a flavin analogue (isoalloxazine) at nanostructured silver surfaces are reported. Unique in the present study, the flavin is not directly adsorbed at the Ag surface but is attached through a chemical reaction between cysteamine adsorbed on the Ag surface and methylformylisoalloxazine. Even though the flavin is held away from direct contact with the metal, strong surface enhancements are observed. The nanostructured silver surfaces are produced by electrodeposition through colloidal templates to produce thin (< 1 μm) films containing close-packed hexagonal arrays of uniform 900 nm sphere segment voids. The sphere segment void (SSV) structured silver surfaces are shown to be ideally suited to in situ electrochemical SERS studies at 633 nm, giving stable, reproducible surface enhancements at a range of electrode potentials, and we show that the SER spectra are sensitive to subfemtomole quantities of immobilized flavin. Studies of the SER spectra as a function of the electrode potential show clear evidence for the formation of the flavin semiquinone at the electrode surface at cathodic potentials. © 2008 American Chemical Society.
format JOUR
author Abdelsalam, M.
Bartlett, P.N.
Russell, A.E.
Baumberg, J.J.
Calvo, E.J.
Tognalli, N.G.
Fainstein, A.
author_facet Abdelsalam, M.
Bartlett, P.N.
Russell, A.E.
Baumberg, J.J.
Calvo, E.J.
Tognalli, N.G.
Fainstein, A.
author_sort Abdelsalam, M.
title Quantitative electrochemical SERS of flavin at a structured silver surface
title_short Quantitative electrochemical SERS of flavin at a structured silver surface
title_full Quantitative electrochemical SERS of flavin at a structured silver surface
title_fullStr Quantitative electrochemical SERS of flavin at a structured silver surface
title_full_unstemmed Quantitative electrochemical SERS of flavin at a structured silver surface
title_sort quantitative electrochemical sers of flavin at a structured silver surface
url http://hdl.handle.net/20.500.12110/paper_07437463_v24_n13_p7018_Abdelsalam
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