Photophysics on surfaces: Determination of absolute fluorescence quantum yields from reflectance spectra

A method for the calculation of absolute fluorescence quantum yields for dyes attached to solid particles based on reflectance measurements is reported. The same procedure allows calculation of true reflectance spectra (free of fluorescence) for highly fluorescent materials as well. Samples of cresy...

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Detalles Bibliográficos
Autores principales: Mirenda, M., Lagorio, M.G., Román, E.S.
Formato: JOUR
Materias:
Cellulose
Diffusion
Fluorescence
Light absorption
Light emission
Light reflection
Light scattering
Photochemical reactions
Spectroscopic analysis
Dye aggregation
Excitation radiation
Fluorescence quantum yields
Stokes shift
Dyes
Light Absorption
Optical Scattering
Photochemistry
Spectrochemical Analysis
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_07437463_v20_n9_p3690_Mirenda
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_07437463_v20_n9_p3690_Mirenda
record_format dspace
spelling todo:paper_07437463_v20_n9_p3690_Mirenda2023-10-03T15:38:36Z Photophysics on surfaces: Determination of absolute fluorescence quantum yields from reflectance spectra Mirenda, M. Lagorio, M.G. Román, E.S. Cellulose Diffusion Fluorescence Light absorption Light emission Light reflection Light scattering Photochemical reactions Spectroscopic analysis Dye aggregation Excitation radiation Fluorescence quantum yields Stokes shift Dyes Cellulose Diffusion Dyes Light Absorption Optical Scattering Photochemistry Spectrochemical Analysis A method for the calculation of absolute fluorescence quantum yields for dyes attached to solid particles based on reflectance measurements is reported. The same procedure allows calculation of true reflectance spectra (free of fluorescence) for highly fluorescent materials as well. Samples of cresyl violet were immobilized by adsorption on microgranular cellulose in the concentration range 4.5 × 10-9 to 3.8 × 10-6 mol g -1. Diffuse and total reflectance spectra were recorded with and without insertion of an optical absorption filter between the output of the integrating sphere of a reflectance spectrometer and the photodetector in order to block fluorescence partially. From these data, the relative emission spectrum of the dye, the filter transmission spectrum, and the detector sensitivity, true reflectances and absolute fluorescence quantum yields were recovered. Observed fluorescence quantum yields, affected by dye aggregation and inner filter effects, were concentration and wavelength dependent, ranging approximately between 0.1 and 0.6. The analysis of remission function spectra showed that dye aggregation is negligible up to a concentration of 1.41 × 10-7 mol g-1. Fluorescence data were corrected for reemission and reabsorption using a suitable model [Lagorio, M. G.; Dicelio, L. E.; Litter, M. I.; San Román, E. J. Chem. Soc., Faraday Trans. 1998, 94, 419]. Application of this model to samples showing no aggregation yielded a wavelength-independent true fluorescence quantum yield of 0.60 ± 0.05, similar to values found in solution. The usage of cresyl violet as a reference for the evaluation of fluorescence quantum yields for weakly fluorescing samples in the solid phase is discussed. Fil:Mirenda, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Lagorio, M.G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_07437463_v20_n9_p3690_Mirenda
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Cellulose
Diffusion
Fluorescence
Light absorption
Light emission
Light reflection
Light scattering
Photochemical reactions
Spectroscopic analysis
Dye aggregation
Excitation radiation
Fluorescence quantum yields
Stokes shift
Dyes
Cellulose
Diffusion
Dyes
Light Absorption
Optical Scattering
Photochemistry
Spectrochemical Analysis
spellingShingle Cellulose
Diffusion
Fluorescence
Light absorption
Light emission
Light reflection
Light scattering
Photochemical reactions
Spectroscopic analysis
Dye aggregation
Excitation radiation
Fluorescence quantum yields
Stokes shift
Dyes
Cellulose
Diffusion
Dyes
Light Absorption
Optical Scattering
Photochemistry
Spectrochemical Analysis
Mirenda, M.
Lagorio, M.G.
Román, E.S.
Photophysics on surfaces: Determination of absolute fluorescence quantum yields from reflectance spectra
topic_facet Cellulose
Diffusion
Fluorescence
Light absorption
Light emission
Light reflection
Light scattering
Photochemical reactions
Spectroscopic analysis
Dye aggregation
Excitation radiation
Fluorescence quantum yields
Stokes shift
Dyes
Cellulose
Diffusion
Dyes
Light Absorption
Optical Scattering
Photochemistry
Spectrochemical Analysis
description A method for the calculation of absolute fluorescence quantum yields for dyes attached to solid particles based on reflectance measurements is reported. The same procedure allows calculation of true reflectance spectra (free of fluorescence) for highly fluorescent materials as well. Samples of cresyl violet were immobilized by adsorption on microgranular cellulose in the concentration range 4.5 × 10-9 to 3.8 × 10-6 mol g -1. Diffuse and total reflectance spectra were recorded with and without insertion of an optical absorption filter between the output of the integrating sphere of a reflectance spectrometer and the photodetector in order to block fluorescence partially. From these data, the relative emission spectrum of the dye, the filter transmission spectrum, and the detector sensitivity, true reflectances and absolute fluorescence quantum yields were recovered. Observed fluorescence quantum yields, affected by dye aggregation and inner filter effects, were concentration and wavelength dependent, ranging approximately between 0.1 and 0.6. The analysis of remission function spectra showed that dye aggregation is negligible up to a concentration of 1.41 × 10-7 mol g-1. Fluorescence data were corrected for reemission and reabsorption using a suitable model [Lagorio, M. G.; Dicelio, L. E.; Litter, M. I.; San Román, E. J. Chem. Soc., Faraday Trans. 1998, 94, 419]. Application of this model to samples showing no aggregation yielded a wavelength-independent true fluorescence quantum yield of 0.60 ± 0.05, similar to values found in solution. The usage of cresyl violet as a reference for the evaluation of fluorescence quantum yields for weakly fluorescing samples in the solid phase is discussed.
format JOUR
author Mirenda, M.
Lagorio, M.G.
Román, E.S.
author_facet Mirenda, M.
Lagorio, M.G.
Román, E.S.
author_sort Mirenda, M.
title Photophysics on surfaces: Determination of absolute fluorescence quantum yields from reflectance spectra
title_short Photophysics on surfaces: Determination of absolute fluorescence quantum yields from reflectance spectra
title_full Photophysics on surfaces: Determination of absolute fluorescence quantum yields from reflectance spectra
title_fullStr Photophysics on surfaces: Determination of absolute fluorescence quantum yields from reflectance spectra
title_full_unstemmed Photophysics on surfaces: Determination of absolute fluorescence quantum yields from reflectance spectra
title_sort photophysics on surfaces: determination of absolute fluorescence quantum yields from reflectance spectra
url http://hdl.handle.net/20.500.12110/paper_07437463_v20_n9_p3690_Mirenda
work_keys_str_mv AT mirendam photophysicsonsurfacesdeterminationofabsolutefluorescencequantumyieldsfromreflectancespectra
AT lagoriomg photophysicsonsurfacesdeterminationofabsolutefluorescencequantumyieldsfromreflectancespectra
AT romanes photophysicsonsurfacesdeterminationofabsolutefluorescencequantumyieldsfromreflectancespectra
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