High δ-aminolevulinic acid uptake in rat cerebral cortex: effect on porphyrin biosynthesis
The response of nerve cells to high exogenous aminolevulinic acid (ALA) concentrations was studied by examining the changes in its uptake and in porphyrin biosynthesis. ALA was shown to be taken up by cerebral cortex particles by a non-saturable process. As opposed to other previously described expe...
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todo:paper_07428413_v111_n1_p143_Juknat2023-10-03T15:38:26Z High δ-aminolevulinic acid uptake in rat cerebral cortex: effect on porphyrin biosynthesis Juknat, A.A. Kotler, M.L. del Carmen Batlle, A.M. Acute porphyric attac AIP brain tissue model ALA accumulation ALA uptake Cerebral cortex particles Glucose uptake PBG accumulation Porphyrin biosynthesis aminolevulinic acid glucose porphyrin animal tissue article bioaccumulation brain cortex controlled study male nerve cell nonhuman porphyria priority journal protein synthesis rat Aminolevulinic Acid Animal Cerebral Cortex Chromatography, High Pressure Liquid Glucose Heme In Vitro Male Porphyrins Rats Spectrometry, Fluorescence Support, Non-U.S. Gov't The response of nerve cells to high exogenous aminolevulinic acid (ALA) concentrations was studied by examining the changes in its uptake and in porphyrin biosynthesis. ALA was shown to be taken up by cerebral cortex particles by a non-saturable process. As opposed to other previously described experimental systems, it was also observed that 84-87% of porphyrins formed was found within the cells. Exposure of cerebral cortex particles to high exogenous ALA (0.8-4.0 mM) showed that ALA can be accumulated in relatively high concentrations in brain cells (21.04 ± 1.05 nmol/mg protein). Under these experimental conditions, porphyrin biosynthesis was found to be markedly inhibited (52%). 2.4 mM ALA caused an initial stimulation of glucose uptake after 1 hr incubation and a later fall to below control values, being consistent with the fact that acute porphyric crisis could be precipitated by the action of ALA on energy metabolism. ALA toxicity could be due both to its accumulation in the cells and to deficient heme concentrations, with an additional effect on glucose metabolism. These findings provide the basis for a useful brain tissue model to investigate the nature of the metabolic mechanisms occurring in acute intermittent porphyria (AIP) patients. © 1995. Fil:Juknat, A.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:del Carmen Batlle, A.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_07428413_v111_n1_p143_Juknat |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Acute porphyric attac AIP brain tissue model ALA accumulation ALA uptake Cerebral cortex particles Glucose uptake PBG accumulation Porphyrin biosynthesis aminolevulinic acid glucose porphyrin animal tissue article bioaccumulation brain cortex controlled study male nerve cell nonhuman porphyria priority journal protein synthesis rat Aminolevulinic Acid Animal Cerebral Cortex Chromatography, High Pressure Liquid Glucose Heme In Vitro Male Porphyrins Rats Spectrometry, Fluorescence Support, Non-U.S. Gov't |
| spellingShingle |
Acute porphyric attac AIP brain tissue model ALA accumulation ALA uptake Cerebral cortex particles Glucose uptake PBG accumulation Porphyrin biosynthesis aminolevulinic acid glucose porphyrin animal tissue article bioaccumulation brain cortex controlled study male nerve cell nonhuman porphyria priority journal protein synthesis rat Aminolevulinic Acid Animal Cerebral Cortex Chromatography, High Pressure Liquid Glucose Heme In Vitro Male Porphyrins Rats Spectrometry, Fluorescence Support, Non-U.S. Gov't Juknat, A.A. Kotler, M.L. del Carmen Batlle, A.M. High δ-aminolevulinic acid uptake in rat cerebral cortex: effect on porphyrin biosynthesis |
| topic_facet |
Acute porphyric attac AIP brain tissue model ALA accumulation ALA uptake Cerebral cortex particles Glucose uptake PBG accumulation Porphyrin biosynthesis aminolevulinic acid glucose porphyrin animal tissue article bioaccumulation brain cortex controlled study male nerve cell nonhuman porphyria priority journal protein synthesis rat Aminolevulinic Acid Animal Cerebral Cortex Chromatography, High Pressure Liquid Glucose Heme In Vitro Male Porphyrins Rats Spectrometry, Fluorescence Support, Non-U.S. Gov't |
| description |
The response of nerve cells to high exogenous aminolevulinic acid (ALA) concentrations was studied by examining the changes in its uptake and in porphyrin biosynthesis. ALA was shown to be taken up by cerebral cortex particles by a non-saturable process. As opposed to other previously described experimental systems, it was also observed that 84-87% of porphyrins formed was found within the cells. Exposure of cerebral cortex particles to high exogenous ALA (0.8-4.0 mM) showed that ALA can be accumulated in relatively high concentrations in brain cells (21.04 ± 1.05 nmol/mg protein). Under these experimental conditions, porphyrin biosynthesis was found to be markedly inhibited (52%). 2.4 mM ALA caused an initial stimulation of glucose uptake after 1 hr incubation and a later fall to below control values, being consistent with the fact that acute porphyric crisis could be precipitated by the action of ALA on energy metabolism. ALA toxicity could be due both to its accumulation in the cells and to deficient heme concentrations, with an additional effect on glucose metabolism. These findings provide the basis for a useful brain tissue model to investigate the nature of the metabolic mechanisms occurring in acute intermittent porphyria (AIP) patients. © 1995. |
| format |
JOUR |
| author |
Juknat, A.A. Kotler, M.L. del Carmen Batlle, A.M. |
| author_facet |
Juknat, A.A. Kotler, M.L. del Carmen Batlle, A.M. |
| author_sort |
Juknat, A.A. |
| title |
High δ-aminolevulinic acid uptake in rat cerebral cortex: effect on porphyrin biosynthesis |
| title_short |
High δ-aminolevulinic acid uptake in rat cerebral cortex: effect on porphyrin biosynthesis |
| title_full |
High δ-aminolevulinic acid uptake in rat cerebral cortex: effect on porphyrin biosynthesis |
| title_fullStr |
High δ-aminolevulinic acid uptake in rat cerebral cortex: effect on porphyrin biosynthesis |
| title_full_unstemmed |
High δ-aminolevulinic acid uptake in rat cerebral cortex: effect on porphyrin biosynthesis |
| title_sort |
high δ-aminolevulinic acid uptake in rat cerebral cortex: effect on porphyrin biosynthesis |
| url |
http://hdl.handle.net/20.500.12110/paper_07428413_v111_n1_p143_Juknat |
| work_keys_str_mv |
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