Diurnal Variations of Benzodiazepine Binding in Rat Cerebral Cortex: Disruption by Pinealectomy
In a previous work, pinealectomy was found to depress benzodiazepine (BZP) receptor binding in cerebral cortex membranes of rats killed at noon. In order to assess the effect of pineal removal on diurnal variations of BZP binding site concentration and affinity, groups of intact, pinealectomized, or...
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todo:paper_07423098_v3_n2_p101_AcunaÀCastroviejo2023-10-03T15:38:25Z Diurnal Variations of Benzodiazepine Binding in Rat Cerebral Cortex: Disruption by Pinealectomy Acuña‐Castroviejo, D. Lowenstein, P.R. Rosenstein, R. Cardinali, D.P. brain benzodiazepine binding diurnal rhythms melatonin pineal gland pinealectomy benzodiazepine receptor flunitrazepam melatonin radioisotope animal cell animal experiment autopsy brain cortex central nervous system circadian rhythm drug administration drug binding drug receptor binding flunitrazepam h 3 methodology nonhuman pharmacokinetics pinealectomy rat Animal Cerebral Cortex Circadian Rhythm Dose-Response Relationship, Drug Flunitrazepam Male Melatonin Membranes Pineal Gland Rats Receptors, GABA-A Support, Non-U.S. Gov't In a previous work, pinealectomy was found to depress benzodiazepine (BZP) receptor binding in cerebral cortex membranes of rats killed at noon. In order to assess the effect of pineal removal on diurnal variations of BZP binding site concentration and affinity, groups of intact, pinealectomized, or sham‐ pinealectomized rats (subjected to surgery 2 wk earlier) were killed at six different time intervals during the 24‐h cycle. BZP binding was assessed by Scatchard analysis of 3H‐flunitrazepam high‐affinity binding to cerebral cortex membranes. In intact and sham‐pinealectomized rats, a maximum in BZP receptor concentration was found at midnight. Pinealectomy blunted the nocturnal peak of receptor concentration and caused a significant depression of binding site number at noon. No changes in the affinity of the binding sites for the radioligand were detected as a function of time of day or following surgery. In a doseresponse experiment for melatonin ability to restore the depressed BZP receptor concentration of cerebral cortex membranes of pinealectomized rats killed at noon, a minimal effective dose of 25 μg/kg body weight was obtained. These results further support a link between pineal activity and brain BZP receptors in rats. Copyright © 1986, Wiley Blackwell. All rights reserved JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_07423098_v3_n2_p101_Acuna‐Castroviejo |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
brain benzodiazepine binding diurnal rhythms melatonin pineal gland pinealectomy benzodiazepine receptor flunitrazepam melatonin radioisotope animal cell animal experiment autopsy brain cortex central nervous system circadian rhythm drug administration drug binding drug receptor binding flunitrazepam h 3 methodology nonhuman pharmacokinetics pinealectomy rat Animal Cerebral Cortex Circadian Rhythm Dose-Response Relationship, Drug Flunitrazepam Male Melatonin Membranes Pineal Gland Rats Receptors, GABA-A Support, Non-U.S. Gov't |
| spellingShingle |
brain benzodiazepine binding diurnal rhythms melatonin pineal gland pinealectomy benzodiazepine receptor flunitrazepam melatonin radioisotope animal cell animal experiment autopsy brain cortex central nervous system circadian rhythm drug administration drug binding drug receptor binding flunitrazepam h 3 methodology nonhuman pharmacokinetics pinealectomy rat Animal Cerebral Cortex Circadian Rhythm Dose-Response Relationship, Drug Flunitrazepam Male Melatonin Membranes Pineal Gland Rats Receptors, GABA-A Support, Non-U.S. Gov't Acuña‐Castroviejo, D. Lowenstein, P.R. Rosenstein, R. Cardinali, D.P. Diurnal Variations of Benzodiazepine Binding in Rat Cerebral Cortex: Disruption by Pinealectomy |
| topic_facet |
brain benzodiazepine binding diurnal rhythms melatonin pineal gland pinealectomy benzodiazepine receptor flunitrazepam melatonin radioisotope animal cell animal experiment autopsy brain cortex central nervous system circadian rhythm drug administration drug binding drug receptor binding flunitrazepam h 3 methodology nonhuman pharmacokinetics pinealectomy rat Animal Cerebral Cortex Circadian Rhythm Dose-Response Relationship, Drug Flunitrazepam Male Melatonin Membranes Pineal Gland Rats Receptors, GABA-A Support, Non-U.S. Gov't |
| description |
In a previous work, pinealectomy was found to depress benzodiazepine (BZP) receptor binding in cerebral cortex membranes of rats killed at noon. In order to assess the effect of pineal removal on diurnal variations of BZP binding site concentration and affinity, groups of intact, pinealectomized, or sham‐ pinealectomized rats (subjected to surgery 2 wk earlier) were killed at six different time intervals during the 24‐h cycle. BZP binding was assessed by Scatchard analysis of 3H‐flunitrazepam high‐affinity binding to cerebral cortex membranes. In intact and sham‐pinealectomized rats, a maximum in BZP receptor concentration was found at midnight. Pinealectomy blunted the nocturnal peak of receptor concentration and caused a significant depression of binding site number at noon. No changes in the affinity of the binding sites for the radioligand were detected as a function of time of day or following surgery. In a doseresponse experiment for melatonin ability to restore the depressed BZP receptor concentration of cerebral cortex membranes of pinealectomized rats killed at noon, a minimal effective dose of 25 μg/kg body weight was obtained. These results further support a link between pineal activity and brain BZP receptors in rats. Copyright © 1986, Wiley Blackwell. All rights reserved |
| format |
JOUR |
| author |
Acuña‐Castroviejo, D. Lowenstein, P.R. Rosenstein, R. Cardinali, D.P. |
| author_facet |
Acuña‐Castroviejo, D. Lowenstein, P.R. Rosenstein, R. Cardinali, D.P. |
| author_sort |
Acuña‐Castroviejo, D. |
| title |
Diurnal Variations of Benzodiazepine Binding in Rat Cerebral Cortex: Disruption by Pinealectomy |
| title_short |
Diurnal Variations of Benzodiazepine Binding in Rat Cerebral Cortex: Disruption by Pinealectomy |
| title_full |
Diurnal Variations of Benzodiazepine Binding in Rat Cerebral Cortex: Disruption by Pinealectomy |
| title_fullStr |
Diurnal Variations of Benzodiazepine Binding in Rat Cerebral Cortex: Disruption by Pinealectomy |
| title_full_unstemmed |
Diurnal Variations of Benzodiazepine Binding in Rat Cerebral Cortex: Disruption by Pinealectomy |
| title_sort |
diurnal variations of benzodiazepine binding in rat cerebral cortex: disruption by pinealectomy |
| url |
http://hdl.handle.net/20.500.12110/paper_07423098_v3_n2_p101_Acuna‐Castroviejo |
| work_keys_str_mv |
AT acunacastroviejod diurnalvariationsofbenzodiazepinebindinginratcerebralcortexdisruptionbypinealectomy AT lowensteinpr diurnalvariationsofbenzodiazepinebindinginratcerebralcortexdisruptionbypinealectomy AT rosensteinr diurnalvariationsofbenzodiazepinebindinginratcerebralcortexdisruptionbypinealectomy AT cardinalidp diurnalvariationsofbenzodiazepinebindinginratcerebralcortexdisruptionbypinealectomy |
| _version_ |
1807316425148203008 |