S-Adenosyl-L-methionine: Its effect on aminolevulinate dehydratase and glutathione in acute ethanol intoxication
The effect of disulfiram and S-adenosyl-L-methionine (SAM) administration to acute ethanol intoxicated mice on the hepatic glutathione (GSH) concentration and aminolevulinic and dehydratase (ALA-D) activity was investigated. It was found that both GSH levels and ALA-D activity were decreased, and ev...
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todo:paper_07418329_v4_n2_p81_Paredes2023-10-03T15:38:21Z S-Adenosyl-L-methionine: Its effect on aminolevulinate dehydratase and glutathione in acute ethanol intoxication Paredes, S.R. Kozicki, P.A. Fukuda, H. Rossetti, M.V. del C. Batlle, A.M. Acetaldehyde Alcohol Glutathione S-Adenosyl-L-methionine δ-Aminolevulinic acid dehydratase alcohol glutathione porphobilinogen synthase s adenosylmethionine animal experiment drug interaction drug toxicity intoxication intraperitoneal drug administration liver liver toxicity mouse nonhuman Acetaldehyde Alcoholic Intoxication Animals Disease Models, Animal Disulfiram Erythrocytes Glutathione Liver Male Mice Porphobilinogen Synthase S-Adenosylmethionine Animalia The effect of disulfiram and S-adenosyl-L-methionine (SAM) administration to acute ethanol intoxicated mice on the hepatic glutathione (GSH) concentration and aminolevulinic and dehydratase (ALA-D) activity was investigated. It was found that both GSH levels and ALA-D activity were decreased, and evidence suggested that the toxic action of ethanol was due to its conversion into acetaldehyde. Administration of SAM reverses the effects of acute alcohol abuse by increasing liver GSH availability. In vitro, hepatic ALA-D activity was not modified by ethanol; instead it was non-competitively inhibited by acetaldehyde. This inhibition was efficiently reversed by GSH and cysteine (CySH). Therefore, a mechanism for the action of ethanol on ALA-D, based on the inhibitory effect of acetaldehyde, is proposed. © 1987. Fil:Paredes, S.R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Fukuda, H. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Rossetti, M.V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:del C. Batlle, A.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_07418329_v4_n2_p81_Paredes |
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Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Acetaldehyde Alcohol Glutathione S-Adenosyl-L-methionine δ-Aminolevulinic acid dehydratase alcohol glutathione porphobilinogen synthase s adenosylmethionine animal experiment drug interaction drug toxicity intoxication intraperitoneal drug administration liver liver toxicity mouse nonhuman Acetaldehyde Alcoholic Intoxication Animals Disease Models, Animal Disulfiram Erythrocytes Glutathione Liver Male Mice Porphobilinogen Synthase S-Adenosylmethionine Animalia |
| spellingShingle |
Acetaldehyde Alcohol Glutathione S-Adenosyl-L-methionine δ-Aminolevulinic acid dehydratase alcohol glutathione porphobilinogen synthase s adenosylmethionine animal experiment drug interaction drug toxicity intoxication intraperitoneal drug administration liver liver toxicity mouse nonhuman Acetaldehyde Alcoholic Intoxication Animals Disease Models, Animal Disulfiram Erythrocytes Glutathione Liver Male Mice Porphobilinogen Synthase S-Adenosylmethionine Animalia Paredes, S.R. Kozicki, P.A. Fukuda, H. Rossetti, M.V. del C. Batlle, A.M. S-Adenosyl-L-methionine: Its effect on aminolevulinate dehydratase and glutathione in acute ethanol intoxication |
| topic_facet |
Acetaldehyde Alcohol Glutathione S-Adenosyl-L-methionine δ-Aminolevulinic acid dehydratase alcohol glutathione porphobilinogen synthase s adenosylmethionine animal experiment drug interaction drug toxicity intoxication intraperitoneal drug administration liver liver toxicity mouse nonhuman Acetaldehyde Alcoholic Intoxication Animals Disease Models, Animal Disulfiram Erythrocytes Glutathione Liver Male Mice Porphobilinogen Synthase S-Adenosylmethionine Animalia |
| description |
The effect of disulfiram and S-adenosyl-L-methionine (SAM) administration to acute ethanol intoxicated mice on the hepatic glutathione (GSH) concentration and aminolevulinic and dehydratase (ALA-D) activity was investigated. It was found that both GSH levels and ALA-D activity were decreased, and evidence suggested that the toxic action of ethanol was due to its conversion into acetaldehyde. Administration of SAM reverses the effects of acute alcohol abuse by increasing liver GSH availability. In vitro, hepatic ALA-D activity was not modified by ethanol; instead it was non-competitively inhibited by acetaldehyde. This inhibition was efficiently reversed by GSH and cysteine (CySH). Therefore, a mechanism for the action of ethanol on ALA-D, based on the inhibitory effect of acetaldehyde, is proposed. © 1987. |
| format |
JOUR |
| author |
Paredes, S.R. Kozicki, P.A. Fukuda, H. Rossetti, M.V. del C. Batlle, A.M. |
| author_facet |
Paredes, S.R. Kozicki, P.A. Fukuda, H. Rossetti, M.V. del C. Batlle, A.M. |
| author_sort |
Paredes, S.R. |
| title |
S-Adenosyl-L-methionine: Its effect on aminolevulinate dehydratase and glutathione in acute ethanol intoxication |
| title_short |
S-Adenosyl-L-methionine: Its effect on aminolevulinate dehydratase and glutathione in acute ethanol intoxication |
| title_full |
S-Adenosyl-L-methionine: Its effect on aminolevulinate dehydratase and glutathione in acute ethanol intoxication |
| title_fullStr |
S-Adenosyl-L-methionine: Its effect on aminolevulinate dehydratase and glutathione in acute ethanol intoxication |
| title_full_unstemmed |
S-Adenosyl-L-methionine: Its effect on aminolevulinate dehydratase and glutathione in acute ethanol intoxication |
| title_sort |
s-adenosyl-l-methionine: its effect on aminolevulinate dehydratase and glutathione in acute ethanol intoxication |
| url |
http://hdl.handle.net/20.500.12110/paper_07418329_v4_n2_p81_Paredes |
| work_keys_str_mv |
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| _version_ |
1807317137805541376 |