Abiotic factors and their interactions influence on the co-production of aflatoxin B1 and cyclopiazonic acid by Aspergillus flavus isolated from corn
The objectives of this study were i) to determine the effects of the interactions of water activity, temperature and incubation time on the co-production of AFB1 and CPA by isolates of Aspergillus flavus with different profile of mycotoxin production and ii) to identify the aW and temperature limiti...
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todo:paper_07400020_v38_n_p276_Astoreca2023-10-03T15:38:06Z Abiotic factors and their interactions influence on the co-production of aflatoxin B1 and cyclopiazonic acid by Aspergillus flavus isolated from corn Astoreca, A. Vaamonde, G. Dalcero, A. Marin, S. Ramos, A. Aflatoxin B1 Aspergillus flavus Cyclopiazonic acid Temperature Water activity Aspergillus flavus Fungi Zea mays aflatoxin B1 cyclopiazonic acid indole derivative Aflatoxin B(1) article Aspergillus flavus biosynthesis chemistry culture medium isolation and purification maize metabolism microbiology temperature water activity Aflatoxin B(1) Aspergillus flavus Cyclopiazonic acid Temperature Water activity Aflatoxin B1 Aspergillus flavus Culture Media Indoles Temperature Zea mays The objectives of this study were i) to determine the effects of the interactions of water activity, temperature and incubation time on the co-production of AFB1 and CPA by isolates of Aspergillus flavus with different profile of mycotoxin production and ii) to identify the aW and temperature limiting conditions for the production of both mycotoxins. Fungi used in this study were selected because they belonged to different chemotypes: chemotype I (AFB1+/CPA+), III (AFB1+/CPA-) and IV (AFB1-/CPA+), respectively. Two culture media were used; Czapek yeast agar (CYA) and corn extract agar (CEM), at different incubated temperatures (10-40°C) and aW levels (0.80-0.98). AFB1 and CPA production were analyzed after 7, 14, 21 and 28 days of incubation. Significant differences were observed with respect to mycotoxin production depending on the media evaluated. The AFB1 production occurred more favorably on CYA while the highest CPA concentrations were recorded on CEM. Within the range of aW evaluated in this study, 0.83 was the limiting level for both toxins production. The optimum conditions for AFB1 production occurred at 0.96 aW and 30°C after 21 days of incubation, regardless of the media and isolate. Although different amounts of toxins were produced in each medium, the limiting and optimum conditions for their production were similar in both. No differences in the response of the three isolates to the abiotic factors discussed were observed despite belonging to different chemotypes. The determination of the thresholds of mycotoxins co-production, especially in the case of data obtained with the corn extract medium can be useful to avoid the conditions conducive to co-occurrence of these mycotoxins in corn. © 2013. Fil:Vaamonde, G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_07400020_v38_n_p276_Astoreca |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Aflatoxin B1 Aspergillus flavus Cyclopiazonic acid Temperature Water activity Aspergillus flavus Fungi Zea mays aflatoxin B1 cyclopiazonic acid indole derivative Aflatoxin B(1) article Aspergillus flavus biosynthesis chemistry culture medium isolation and purification maize metabolism microbiology temperature water activity Aflatoxin B(1) Aspergillus flavus Cyclopiazonic acid Temperature Water activity Aflatoxin B1 Aspergillus flavus Culture Media Indoles Temperature Zea mays |
| spellingShingle |
Aflatoxin B1 Aspergillus flavus Cyclopiazonic acid Temperature Water activity Aspergillus flavus Fungi Zea mays aflatoxin B1 cyclopiazonic acid indole derivative Aflatoxin B(1) article Aspergillus flavus biosynthesis chemistry culture medium isolation and purification maize metabolism microbiology temperature water activity Aflatoxin B(1) Aspergillus flavus Cyclopiazonic acid Temperature Water activity Aflatoxin B1 Aspergillus flavus Culture Media Indoles Temperature Zea mays Astoreca, A. Vaamonde, G. Dalcero, A. Marin, S. Ramos, A. Abiotic factors and their interactions influence on the co-production of aflatoxin B1 and cyclopiazonic acid by Aspergillus flavus isolated from corn |
| topic_facet |
Aflatoxin B1 Aspergillus flavus Cyclopiazonic acid Temperature Water activity Aspergillus flavus Fungi Zea mays aflatoxin B1 cyclopiazonic acid indole derivative Aflatoxin B(1) article Aspergillus flavus biosynthesis chemistry culture medium isolation and purification maize metabolism microbiology temperature water activity Aflatoxin B(1) Aspergillus flavus Cyclopiazonic acid Temperature Water activity Aflatoxin B1 Aspergillus flavus Culture Media Indoles Temperature Zea mays |
| description |
The objectives of this study were i) to determine the effects of the interactions of water activity, temperature and incubation time on the co-production of AFB1 and CPA by isolates of Aspergillus flavus with different profile of mycotoxin production and ii) to identify the aW and temperature limiting conditions for the production of both mycotoxins. Fungi used in this study were selected because they belonged to different chemotypes: chemotype I (AFB1+/CPA+), III (AFB1+/CPA-) and IV (AFB1-/CPA+), respectively. Two culture media were used; Czapek yeast agar (CYA) and corn extract agar (CEM), at different incubated temperatures (10-40°C) and aW levels (0.80-0.98). AFB1 and CPA production were analyzed after 7, 14, 21 and 28 days of incubation. Significant differences were observed with respect to mycotoxin production depending on the media evaluated. The AFB1 production occurred more favorably on CYA while the highest CPA concentrations were recorded on CEM. Within the range of aW evaluated in this study, 0.83 was the limiting level for both toxins production. The optimum conditions for AFB1 production occurred at 0.96 aW and 30°C after 21 days of incubation, regardless of the media and isolate. Although different amounts of toxins were produced in each medium, the limiting and optimum conditions for their production were similar in both. No differences in the response of the three isolates to the abiotic factors discussed were observed despite belonging to different chemotypes. The determination of the thresholds of mycotoxins co-production, especially in the case of data obtained with the corn extract medium can be useful to avoid the conditions conducive to co-occurrence of these mycotoxins in corn. © 2013. |
| format |
JOUR |
| author |
Astoreca, A. Vaamonde, G. Dalcero, A. Marin, S. Ramos, A. |
| author_facet |
Astoreca, A. Vaamonde, G. Dalcero, A. Marin, S. Ramos, A. |
| author_sort |
Astoreca, A. |
| title |
Abiotic factors and their interactions influence on the co-production of aflatoxin B1 and cyclopiazonic acid by Aspergillus flavus isolated from corn |
| title_short |
Abiotic factors and their interactions influence on the co-production of aflatoxin B1 and cyclopiazonic acid by Aspergillus flavus isolated from corn |
| title_full |
Abiotic factors and their interactions influence on the co-production of aflatoxin B1 and cyclopiazonic acid by Aspergillus flavus isolated from corn |
| title_fullStr |
Abiotic factors and their interactions influence on the co-production of aflatoxin B1 and cyclopiazonic acid by Aspergillus flavus isolated from corn |
| title_full_unstemmed |
Abiotic factors and their interactions influence on the co-production of aflatoxin B1 and cyclopiazonic acid by Aspergillus flavus isolated from corn |
| title_sort |
abiotic factors and their interactions influence on the co-production of aflatoxin b1 and cyclopiazonic acid by aspergillus flavus isolated from corn |
| url |
http://hdl.handle.net/20.500.12110/paper_07400020_v38_n_p276_Astoreca |
| work_keys_str_mv |
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