Characterisation of an antiviral pediocin-like bacteriocin produced by Enterococcus faecium
The bacteriocin-producing strain Enterococcus faecium ST5Ha was isolated from smoked salmon and identified by biomolecular techniques. Ent. faecium ST5Ha produces a pediocin-like bacteriocin with activity against several lactic acid bacteria, Listeria spp. and some other human and food pathogens, an...
Guardado en:
| Autores principales: | , , , , , , , , |
|---|---|
| Formato: | JOUR |
| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_07400020_v27_n7_p869_Todorov |
| Aporte de: |
| id |
todo:paper_07400020_v27_n7_p869_Todorov |
|---|---|
| record_format |
dspace |
| spelling |
todo:paper_07400020_v27_n7_p869_Todorov2023-10-03T15:38:05Z Characterisation of an antiviral pediocin-like bacteriocin produced by Enterococcus faecium Todorov, S.D. Wachsman, M. Tomé, E. Dousset, X. Destro, M.T. Dicks, L.M.T. de Melo Franco, B.D.G. Vaz-Velho, M. Drider, D. Anti-Listeria activity Antiviral activity Enterococcus faecium Pediocin-like bacteriocin Salmon antiinfective agent antivirus agent bacteriocin animal antibiosis article bacterial count biosynthesis drug effect Enterococcus faecium food control growth, development and aging Herpes simplex virus 1 human Listeria Listeria monocytogenes metabolism microbiological examination microbiology molecular weight physiology product safety salmon sea food Animals Anti-Bacterial Agents Antibiosis Antiviral Agents Bacteriocins Colony Count, Microbial Consumer Product Safety Enterococcus faecium Food Microbiology Herpesvirus 1, Human Humans Listeria Listeria monocytogenes Microbial Sensitivity Tests Molecular Weight Salmon Seafood Bacteria (microorganisms) Enterococcus faecium Human herpesvirus 1 Listeria Listeria ivanovii Listeria monocytogenes The bacteriocin-producing strain Enterococcus faecium ST5Ha was isolated from smoked salmon and identified by biomolecular techniques. Ent. faecium ST5Ha produces a pediocin-like bacteriocin with activity against several lactic acid bacteria, Listeria spp. and some other human and food pathogens, and remarkably against HSV-1 virus. Bacteriocin ST5Ha was produced at high levels in MRS broth at 30 °C and 37 °C, reaching a maximum production of 1.0 × 109 AU/ml, checked against Listeria ivanovii ATCC19119 as target strain and surrogate of pathogenic strain Listeria monocytogenes. The molecular weight of bacteriocin ST5Ha was estimated to be 4.5 kDa according to tricine-SDS-PAGE data. Ent. faecium ST5Ha harbors a 1.044 kb chromosomal DNA fragment fitting in size to that of pediocin PA-1/AcH. In addition, the sequencing of bacteriocin ST5Ha gene indicated 99% of DNA homology to pediocin PA-1/AcH. The combined application of low levels (below MIC) of ciprofloxacin and bacteriocin ST5Ha resulted in a synergetic effect in the inhibition of target strain L. ivanovii ATCC19119. Bacteriocin ST5Ha displayed antiviral activity against HSV-1, an important human pathogen, with a selectivity index of 173. To the best of our knowledge, this is the first report on Ent. faecium as a potential producer of pediocin-like bacteriocin with antiviral activity. © 2010 Elsevier Ltd. Fil:Wachsman, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_07400020_v27_n7_p869_Todorov |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Anti-Listeria activity Antiviral activity Enterococcus faecium Pediocin-like bacteriocin Salmon antiinfective agent antivirus agent bacteriocin animal antibiosis article bacterial count biosynthesis drug effect Enterococcus faecium food control growth, development and aging Herpes simplex virus 1 human Listeria Listeria monocytogenes metabolism microbiological examination microbiology molecular weight physiology product safety salmon sea food Animals Anti-Bacterial Agents Antibiosis Antiviral Agents Bacteriocins Colony Count, Microbial Consumer Product Safety Enterococcus faecium Food Microbiology Herpesvirus 1, Human Humans Listeria Listeria monocytogenes Microbial Sensitivity Tests Molecular Weight Salmon Seafood Bacteria (microorganisms) Enterococcus faecium Human herpesvirus 1 Listeria Listeria ivanovii Listeria monocytogenes |
| spellingShingle |
Anti-Listeria activity Antiviral activity Enterococcus faecium Pediocin-like bacteriocin Salmon antiinfective agent antivirus agent bacteriocin animal antibiosis article bacterial count biosynthesis drug effect Enterococcus faecium food control growth, development and aging Herpes simplex virus 1 human Listeria Listeria monocytogenes metabolism microbiological examination microbiology molecular weight physiology product safety salmon sea food Animals Anti-Bacterial Agents Antibiosis Antiviral Agents Bacteriocins Colony Count, Microbial Consumer Product Safety Enterococcus faecium Food Microbiology Herpesvirus 1, Human Humans Listeria Listeria monocytogenes Microbial Sensitivity Tests Molecular Weight Salmon Seafood Bacteria (microorganisms) Enterococcus faecium Human herpesvirus 1 Listeria Listeria ivanovii Listeria monocytogenes Todorov, S.D. Wachsman, M. Tomé, E. Dousset, X. Destro, M.T. Dicks, L.M.T. de Melo Franco, B.D.G. Vaz-Velho, M. Drider, D. Characterisation of an antiviral pediocin-like bacteriocin produced by Enterococcus faecium |
| topic_facet |
Anti-Listeria activity Antiviral activity Enterococcus faecium Pediocin-like bacteriocin Salmon antiinfective agent antivirus agent bacteriocin animal antibiosis article bacterial count biosynthesis drug effect Enterococcus faecium food control growth, development and aging Herpes simplex virus 1 human Listeria Listeria monocytogenes metabolism microbiological examination microbiology molecular weight physiology product safety salmon sea food Animals Anti-Bacterial Agents Antibiosis Antiviral Agents Bacteriocins Colony Count, Microbial Consumer Product Safety Enterococcus faecium Food Microbiology Herpesvirus 1, Human Humans Listeria Listeria monocytogenes Microbial Sensitivity Tests Molecular Weight Salmon Seafood Bacteria (microorganisms) Enterococcus faecium Human herpesvirus 1 Listeria Listeria ivanovii Listeria monocytogenes |
| description |
The bacteriocin-producing strain Enterococcus faecium ST5Ha was isolated from smoked salmon and identified by biomolecular techniques. Ent. faecium ST5Ha produces a pediocin-like bacteriocin with activity against several lactic acid bacteria, Listeria spp. and some other human and food pathogens, and remarkably against HSV-1 virus. Bacteriocin ST5Ha was produced at high levels in MRS broth at 30 °C and 37 °C, reaching a maximum production of 1.0 × 109 AU/ml, checked against Listeria ivanovii ATCC19119 as target strain and surrogate of pathogenic strain Listeria monocytogenes. The molecular weight of bacteriocin ST5Ha was estimated to be 4.5 kDa according to tricine-SDS-PAGE data. Ent. faecium ST5Ha harbors a 1.044 kb chromosomal DNA fragment fitting in size to that of pediocin PA-1/AcH. In addition, the sequencing of bacteriocin ST5Ha gene indicated 99% of DNA homology to pediocin PA-1/AcH. The combined application of low levels (below MIC) of ciprofloxacin and bacteriocin ST5Ha resulted in a synergetic effect in the inhibition of target strain L. ivanovii ATCC19119. Bacteriocin ST5Ha displayed antiviral activity against HSV-1, an important human pathogen, with a selectivity index of 173. To the best of our knowledge, this is the first report on Ent. faecium as a potential producer of pediocin-like bacteriocin with antiviral activity. © 2010 Elsevier Ltd. |
| format |
JOUR |
| author |
Todorov, S.D. Wachsman, M. Tomé, E. Dousset, X. Destro, M.T. Dicks, L.M.T. de Melo Franco, B.D.G. Vaz-Velho, M. Drider, D. |
| author_facet |
Todorov, S.D. Wachsman, M. Tomé, E. Dousset, X. Destro, M.T. Dicks, L.M.T. de Melo Franco, B.D.G. Vaz-Velho, M. Drider, D. |
| author_sort |
Todorov, S.D. |
| title |
Characterisation of an antiviral pediocin-like bacteriocin produced by Enterococcus faecium |
| title_short |
Characterisation of an antiviral pediocin-like bacteriocin produced by Enterococcus faecium |
| title_full |
Characterisation of an antiviral pediocin-like bacteriocin produced by Enterococcus faecium |
| title_fullStr |
Characterisation of an antiviral pediocin-like bacteriocin produced by Enterococcus faecium |
| title_full_unstemmed |
Characterisation of an antiviral pediocin-like bacteriocin produced by Enterococcus faecium |
| title_sort |
characterisation of an antiviral pediocin-like bacteriocin produced by enterococcus faecium |
| url |
http://hdl.handle.net/20.500.12110/paper_07400020_v27_n7_p869_Todorov |
| work_keys_str_mv |
AT todorovsd characterisationofanantiviralpediocinlikebacteriocinproducedbyenterococcusfaecium AT wachsmanm characterisationofanantiviralpediocinlikebacteriocinproducedbyenterococcusfaecium AT tomee characterisationofanantiviralpediocinlikebacteriocinproducedbyenterococcusfaecium AT doussetx characterisationofanantiviralpediocinlikebacteriocinproducedbyenterococcusfaecium AT destromt characterisationofanantiviralpediocinlikebacteriocinproducedbyenterococcusfaecium AT dickslmt characterisationofanantiviralpediocinlikebacteriocinproducedbyenterococcusfaecium AT demelofrancobdg characterisationofanantiviralpediocinlikebacteriocinproducedbyenterococcusfaecium AT vazvelhom characterisationofanantiviralpediocinlikebacteriocinproducedbyenterococcusfaecium AT driderd characterisationofanantiviralpediocinlikebacteriocinproducedbyenterococcusfaecium |
| _version_ |
1807317640203468800 |