Identification of immunoreactive mammalian gonadotropin-releasing hormone in the brain of metamorphic larvae of Bufo arenarum hensel (Amphibia: Anura)
Gonadotropin-releasing hormone (GnRH) immunoreactivity in brain extracts of Bufo arenarum tadpoles were investigated by high-performance liquid chromatography, followed by radioimmunoassay analysis using two different antisera raised against different GnRH variants. Only one immunoreactive peak was...
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todo:paper_07365748_v14_n5_p663_Somoza2023-10-03T15:37:49Z Identification of immunoreactive mammalian gonadotropin-releasing hormone in the brain of metamorphic larvae of Bufo arenarum hensel (Amphibia: Anura) Somoza, G.M. Paz, D.A. Stefano, A.V. Affanni, J.M. GmRH Migration Nervous terminalis Olfactory placode Tadpoles gonadorelin peptide animal tissue article brain development controlled study dilution forebrain high performance liquid chromatography hypothalamus immunocytochemistry immunoreactivity larva mesencephalon metamorphosis nerve fiber newborn nonhuman olfactory epithelium olfactory nerve perikaryon preoptic area priority journal radioimmunoassay rhombencephalon spinal cord toad Amphibia Animalia Anura Bufo arenarum Mammalia Terminalis Gonadotropin-releasing hormone (GnRH) immunoreactivity in brain extracts of Bufo arenarum tadpoles were investigated by high-performance liquid chromatography, followed by radioimmunoassay analysis using two different antisera raised against different GnRH variants. Only one immunoreactive peak was identified, eluting in the same position as synthetic mammalian GnRH. This result was further confirmed by serial dilution studies using more specific mammalian GnRH antisera. Our results suggest that mammalian GnRH is most likely an endogenous peptide in the brain of the developing larvae and froglets of Bufo arenarum and quite likely it is the only GnRH variant present during those development stages. The distribution and density of cell bodies and fibers were analysed by immunocytochemical procedures. Immunoreactive cell bodies appeared in the olfactory epithelium and across the olfactory nerve at late prometamorphic larval stages. Near the metamorphic climax and in froglets, perikarya and fibers were detected in basal forebrain, preoptic and hypothalamic areas. No immunoreaction was observed at midbrain, hindbrain and spinal cord levels. This study suggests that mammalian GnRH is most likely an endogenous peptide and is probably the only GnRH variant in the brain of the developing larvae and froglets of Bufo arenarum. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_07365748_v14_n5_p663_Somoza |
| institution |
Universidad de Buenos Aires |
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I-28 |
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R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
GmRH Migration Nervous terminalis Olfactory placode Tadpoles gonadorelin peptide animal tissue article brain development controlled study dilution forebrain high performance liquid chromatography hypothalamus immunocytochemistry immunoreactivity larva mesencephalon metamorphosis nerve fiber newborn nonhuman olfactory epithelium olfactory nerve perikaryon preoptic area priority journal radioimmunoassay rhombencephalon spinal cord toad Amphibia Animalia Anura Bufo arenarum Mammalia Terminalis |
| spellingShingle |
GmRH Migration Nervous terminalis Olfactory placode Tadpoles gonadorelin peptide animal tissue article brain development controlled study dilution forebrain high performance liquid chromatography hypothalamus immunocytochemistry immunoreactivity larva mesencephalon metamorphosis nerve fiber newborn nonhuman olfactory epithelium olfactory nerve perikaryon preoptic area priority journal radioimmunoassay rhombencephalon spinal cord toad Amphibia Animalia Anura Bufo arenarum Mammalia Terminalis Somoza, G.M. Paz, D.A. Stefano, A.V. Affanni, J.M. Identification of immunoreactive mammalian gonadotropin-releasing hormone in the brain of metamorphic larvae of Bufo arenarum hensel (Amphibia: Anura) |
| topic_facet |
GmRH Migration Nervous terminalis Olfactory placode Tadpoles gonadorelin peptide animal tissue article brain development controlled study dilution forebrain high performance liquid chromatography hypothalamus immunocytochemistry immunoreactivity larva mesencephalon metamorphosis nerve fiber newborn nonhuman olfactory epithelium olfactory nerve perikaryon preoptic area priority journal radioimmunoassay rhombencephalon spinal cord toad Amphibia Animalia Anura Bufo arenarum Mammalia Terminalis |
| description |
Gonadotropin-releasing hormone (GnRH) immunoreactivity in brain extracts of Bufo arenarum tadpoles were investigated by high-performance liquid chromatography, followed by radioimmunoassay analysis using two different antisera raised against different GnRH variants. Only one immunoreactive peak was identified, eluting in the same position as synthetic mammalian GnRH. This result was further confirmed by serial dilution studies using more specific mammalian GnRH antisera. Our results suggest that mammalian GnRH is most likely an endogenous peptide in the brain of the developing larvae and froglets of Bufo arenarum and quite likely it is the only GnRH variant present during those development stages. The distribution and density of cell bodies and fibers were analysed by immunocytochemical procedures. Immunoreactive cell bodies appeared in the olfactory epithelium and across the olfactory nerve at late prometamorphic larval stages. Near the metamorphic climax and in froglets, perikarya and fibers were detected in basal forebrain, preoptic and hypothalamic areas. No immunoreaction was observed at midbrain, hindbrain and spinal cord levels. This study suggests that mammalian GnRH is most likely an endogenous peptide and is probably the only GnRH variant in the brain of the developing larvae and froglets of Bufo arenarum. |
| format |
JOUR |
| author |
Somoza, G.M. Paz, D.A. Stefano, A.V. Affanni, J.M. |
| author_facet |
Somoza, G.M. Paz, D.A. Stefano, A.V. Affanni, J.M. |
| author_sort |
Somoza, G.M. |
| title |
Identification of immunoreactive mammalian gonadotropin-releasing hormone in the brain of metamorphic larvae of Bufo arenarum hensel (Amphibia: Anura) |
| title_short |
Identification of immunoreactive mammalian gonadotropin-releasing hormone in the brain of metamorphic larvae of Bufo arenarum hensel (Amphibia: Anura) |
| title_full |
Identification of immunoreactive mammalian gonadotropin-releasing hormone in the brain of metamorphic larvae of Bufo arenarum hensel (Amphibia: Anura) |
| title_fullStr |
Identification of immunoreactive mammalian gonadotropin-releasing hormone in the brain of metamorphic larvae of Bufo arenarum hensel (Amphibia: Anura) |
| title_full_unstemmed |
Identification of immunoreactive mammalian gonadotropin-releasing hormone in the brain of metamorphic larvae of Bufo arenarum hensel (Amphibia: Anura) |
| title_sort |
identification of immunoreactive mammalian gonadotropin-releasing hormone in the brain of metamorphic larvae of bufo arenarum hensel (amphibia: anura) |
| url |
http://hdl.handle.net/20.500.12110/paper_07365748_v14_n5_p663_Somoza |
| work_keys_str_mv |
AT somozagm identificationofimmunoreactivemammaliangonadotropinreleasinghormoneinthebrainofmetamorphiclarvaeofbufoarenarumhenselamphibiaanura AT pazda identificationofimmunoreactivemammaliangonadotropinreleasinghormoneinthebrainofmetamorphiclarvaeofbufoarenarumhenselamphibiaanura AT stefanoav identificationofimmunoreactivemammaliangonadotropinreleasinghormoneinthebrainofmetamorphiclarvaeofbufoarenarumhenselamphibiaanura AT affannijm identificationofimmunoreactivemammaliangonadotropinreleasinghormoneinthebrainofmetamorphiclarvaeofbufoarenarumhenselamphibiaanura |
| _version_ |
1807315140461199360 |