Evaluation of Toxoplasma gondii recombinant proteins for the diagnosis of recently acquired toxoplasmosis by an immunoglobulin G analysis
The value of T. gondii recombinant antigens rRop2, rGra4, rGra7 and rSAG1m (mature version) or rSAG1ct (C-terminal version) in differentiating recently acquired from chronic infections was determined by IgG-ELISA. The general highest sensitivity was observed with rRop2 whereas rSAG1m was not recogni...
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_07328893_v47_n4_p609_Nigro |
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todo:paper_07328893_v47_n4_p609_Nigro2023-10-03T15:37:35Z Evaluation of Toxoplasma gondii recombinant proteins for the diagnosis of recently acquired toxoplasmosis by an immunoglobulin G analysis Nigro, M. Gutierrez, A. Hoffer, A.M. Clemente, M. Kaufer, F. Carral, L. Martin, V. Guarnera, E.A. Angel, S.O. immunoglobulin antibody immunoglobulin G parasite antigen protozoal protein recombinant antigen recombinant protein rgra4 antigen rgra7 antigen rrop2 antigen rsag1 antigen unclassified drug antibody detection antigen detection antigenicity article blood sampling carboxy terminal sequence chronic disease controlled study diagnostic accuracy diagnostic value enzyme linked immunosorbent assay evaluation female human intermethod comparison nonhuman parasite identification priority journal statistical significance Toxoplasma gondii toxoplasmosis The value of T. gondii recombinant antigens rRop2, rGra4, rGra7 and rSAG1m (mature version) or rSAG1ct (C-terminal version) in differentiating recently acquired from chronic infections was determined by IgG-ELISA. The general highest sensitivity was observed with rRop2 whereas rSAG1m was not recognized by any of the serum samples, suggesting an incorrect folding. rGra4 and rGra7 showed significant higher sensitivity and absorbance values with serum samples from recently infected individuals compared to those with chronic infection. In contrast, rRop2 and rSAG1ct did not show differences in the reactivity pattern between both groups of serum samples. © 2003 Elsevier Inc. All rights reserved. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_07328893_v47_n4_p609_Nigro |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
immunoglobulin antibody immunoglobulin G parasite antigen protozoal protein recombinant antigen recombinant protein rgra4 antigen rgra7 antigen rrop2 antigen rsag1 antigen unclassified drug antibody detection antigen detection antigenicity article blood sampling carboxy terminal sequence chronic disease controlled study diagnostic accuracy diagnostic value enzyme linked immunosorbent assay evaluation female human intermethod comparison nonhuman parasite identification priority journal statistical significance Toxoplasma gondii toxoplasmosis |
| spellingShingle |
immunoglobulin antibody immunoglobulin G parasite antigen protozoal protein recombinant antigen recombinant protein rgra4 antigen rgra7 antigen rrop2 antigen rsag1 antigen unclassified drug antibody detection antigen detection antigenicity article blood sampling carboxy terminal sequence chronic disease controlled study diagnostic accuracy diagnostic value enzyme linked immunosorbent assay evaluation female human intermethod comparison nonhuman parasite identification priority journal statistical significance Toxoplasma gondii toxoplasmosis Nigro, M. Gutierrez, A. Hoffer, A.M. Clemente, M. Kaufer, F. Carral, L. Martin, V. Guarnera, E.A. Angel, S.O. Evaluation of Toxoplasma gondii recombinant proteins for the diagnosis of recently acquired toxoplasmosis by an immunoglobulin G analysis |
| topic_facet |
immunoglobulin antibody immunoglobulin G parasite antigen protozoal protein recombinant antigen recombinant protein rgra4 antigen rgra7 antigen rrop2 antigen rsag1 antigen unclassified drug antibody detection antigen detection antigenicity article blood sampling carboxy terminal sequence chronic disease controlled study diagnostic accuracy diagnostic value enzyme linked immunosorbent assay evaluation female human intermethod comparison nonhuman parasite identification priority journal statistical significance Toxoplasma gondii toxoplasmosis |
| description |
The value of T. gondii recombinant antigens rRop2, rGra4, rGra7 and rSAG1m (mature version) or rSAG1ct (C-terminal version) in differentiating recently acquired from chronic infections was determined by IgG-ELISA. The general highest sensitivity was observed with rRop2 whereas rSAG1m was not recognized by any of the serum samples, suggesting an incorrect folding. rGra4 and rGra7 showed significant higher sensitivity and absorbance values with serum samples from recently infected individuals compared to those with chronic infection. In contrast, rRop2 and rSAG1ct did not show differences in the reactivity pattern between both groups of serum samples. © 2003 Elsevier Inc. All rights reserved. |
| format |
JOUR |
| author |
Nigro, M. Gutierrez, A. Hoffer, A.M. Clemente, M. Kaufer, F. Carral, L. Martin, V. Guarnera, E.A. Angel, S.O. |
| author_facet |
Nigro, M. Gutierrez, A. Hoffer, A.M. Clemente, M. Kaufer, F. Carral, L. Martin, V. Guarnera, E.A. Angel, S.O. |
| author_sort |
Nigro, M. |
| title |
Evaluation of Toxoplasma gondii recombinant proteins for the diagnosis of recently acquired toxoplasmosis by an immunoglobulin G analysis |
| title_short |
Evaluation of Toxoplasma gondii recombinant proteins for the diagnosis of recently acquired toxoplasmosis by an immunoglobulin G analysis |
| title_full |
Evaluation of Toxoplasma gondii recombinant proteins for the diagnosis of recently acquired toxoplasmosis by an immunoglobulin G analysis |
| title_fullStr |
Evaluation of Toxoplasma gondii recombinant proteins for the diagnosis of recently acquired toxoplasmosis by an immunoglobulin G analysis |
| title_full_unstemmed |
Evaluation of Toxoplasma gondii recombinant proteins for the diagnosis of recently acquired toxoplasmosis by an immunoglobulin G analysis |
| title_sort |
evaluation of toxoplasma gondii recombinant proteins for the diagnosis of recently acquired toxoplasmosis by an immunoglobulin g analysis |
| url |
http://hdl.handle.net/20.500.12110/paper_07328893_v47_n4_p609_Nigro |
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