Tumor necrosis factor alpha induces LIF expression through ERK1/2 activation in mammary epithelial cells
It has been reported that expression of tumor necrosis factor superfamily members occur at the onset of the mammary gland post-lactational involution. One of these proteins, tumor necrosis factor alpha (TNFα), is a major mediator of inflammation that is able to induce expression of several cytokines...
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todo:paper_07302312_v110_n4_p857_Levy2023-10-03T15:37:15Z Tumor necrosis factor alpha induces LIF expression through ERK1/2 activation in mammary epithelial cells Levy, C.S. Slomiansky, V. Gattelli, A. Nahmod, K. Pelisch, F. Blaustein, M. Srebrow, A. Coso, O.A. Kordon, E.C. AP-1 Involution LIF Mammary epithelial cells Mammary gland TNFα leukemia inhibitory factor mitogen activated protein kinase 1 mitogen activated protein kinase 3 transcription factor AP 1 tumor necrosis factor alpha tumor necrosis factor alpha receptor tumor necrosis factor alpha receptor 2 unclassified drug leukemia inhibitory factor LIF protein, human MAPK1 protein, human mitogen activated protein kinase 1 mitogen activated protein kinase 3 transcription factor AP 1 tumor necrosis factor alpha animal cell animal experiment article breast epithelium controlled study enzyme activation lactation mouse nonhuman priority journal protein expression protein induction animal Bagg albino mouse cell line cytology enzymology gel mobility shift assay human immunohistochemistry mammary gland metabolism physiology Western blotting Animals Blotting, Western Cell Line Electrophoretic Mobility Shift Assay Enzyme Activation Humans Immunohistochemistry Leukemia Inhibitory Factor Mammary Glands, Human Mice Mice, Inbred BALB C Mitogen-Activated Protein Kinase 1 Mitogen-Activated Protein Kinase 3 Transcription Factor AP-1 Tumor Necrosis Factor-alpha It has been reported that expression of tumor necrosis factor superfamily members occur at the onset of the mammary gland post-lactational involution. One of these proteins, tumor necrosis factor alpha (TNFα), is a major mediator of inflammation that is able to induce expression of several cytokines. Leukemia inhibitory factor (LIF) is an inflammatory cytokine that is induced and plays a fundamental role during postlactational involution of the mammary gland. Therefore, our goal was to determine whether TNFα activity in the mammary epithelium might include regulation of LIF expression. This biological role would increase the significance of TNFα expression at the end of lactation. Our results show that TNFα was able to induce LIF transcription through ERK1/2 activation in a non-tumorigenic mouse mammary epithelial cell line, SCp2. We found that activation of TNFα receptor-2 (TNFR2) was specifically involved in triggering this signaling pathway. In addition, our data suggest the participation of AP-1 transcription factor family members in this pathway. We determined that TNFα treatment induced c-fos transcription, and blocking AP-1 activity resulted in a significant inhibition of TNFα-induced LIF expression. Finally, we found that TNFα was also able to trigger LIF expression and ERK1/2 activation in the mouse mammary gland in vivo. Therefore, our data suggest that TNFα may contribute to mammary gland involution by, among other activities, eliciting LIF expression through ERK1/2 and AP1 activation. © 2010 Wiley-Liss, Inc. Fil:Slomiansky, V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Pelisch, F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Blaustein, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Srebrow, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Coso, O.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Kordon, E.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_07302312_v110_n4_p857_Levy |
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Universidad de Buenos Aires |
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I-28 |
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R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
AP-1 Involution LIF Mammary epithelial cells Mammary gland TNFα leukemia inhibitory factor mitogen activated protein kinase 1 mitogen activated protein kinase 3 transcription factor AP 1 tumor necrosis factor alpha tumor necrosis factor alpha receptor tumor necrosis factor alpha receptor 2 unclassified drug leukemia inhibitory factor LIF protein, human MAPK1 protein, human mitogen activated protein kinase 1 mitogen activated protein kinase 3 transcription factor AP 1 tumor necrosis factor alpha animal cell animal experiment article breast epithelium controlled study enzyme activation lactation mouse nonhuman priority journal protein expression protein induction animal Bagg albino mouse cell line cytology enzymology gel mobility shift assay human immunohistochemistry mammary gland metabolism physiology Western blotting Animals Blotting, Western Cell Line Electrophoretic Mobility Shift Assay Enzyme Activation Humans Immunohistochemistry Leukemia Inhibitory Factor Mammary Glands, Human Mice Mice, Inbred BALB C Mitogen-Activated Protein Kinase 1 Mitogen-Activated Protein Kinase 3 Transcription Factor AP-1 Tumor Necrosis Factor-alpha |
| spellingShingle |
AP-1 Involution LIF Mammary epithelial cells Mammary gland TNFα leukemia inhibitory factor mitogen activated protein kinase 1 mitogen activated protein kinase 3 transcription factor AP 1 tumor necrosis factor alpha tumor necrosis factor alpha receptor tumor necrosis factor alpha receptor 2 unclassified drug leukemia inhibitory factor LIF protein, human MAPK1 protein, human mitogen activated protein kinase 1 mitogen activated protein kinase 3 transcription factor AP 1 tumor necrosis factor alpha animal cell animal experiment article breast epithelium controlled study enzyme activation lactation mouse nonhuman priority journal protein expression protein induction animal Bagg albino mouse cell line cytology enzymology gel mobility shift assay human immunohistochemistry mammary gland metabolism physiology Western blotting Animals Blotting, Western Cell Line Electrophoretic Mobility Shift Assay Enzyme Activation Humans Immunohistochemistry Leukemia Inhibitory Factor Mammary Glands, Human Mice Mice, Inbred BALB C Mitogen-Activated Protein Kinase 1 Mitogen-Activated Protein Kinase 3 Transcription Factor AP-1 Tumor Necrosis Factor-alpha Levy, C.S. Slomiansky, V. Gattelli, A. Nahmod, K. Pelisch, F. Blaustein, M. Srebrow, A. Coso, O.A. Kordon, E.C. Tumor necrosis factor alpha induces LIF expression through ERK1/2 activation in mammary epithelial cells |
| topic_facet |
AP-1 Involution LIF Mammary epithelial cells Mammary gland TNFα leukemia inhibitory factor mitogen activated protein kinase 1 mitogen activated protein kinase 3 transcription factor AP 1 tumor necrosis factor alpha tumor necrosis factor alpha receptor tumor necrosis factor alpha receptor 2 unclassified drug leukemia inhibitory factor LIF protein, human MAPK1 protein, human mitogen activated protein kinase 1 mitogen activated protein kinase 3 transcription factor AP 1 tumor necrosis factor alpha animal cell animal experiment article breast epithelium controlled study enzyme activation lactation mouse nonhuman priority journal protein expression protein induction animal Bagg albino mouse cell line cytology enzymology gel mobility shift assay human immunohistochemistry mammary gland metabolism physiology Western blotting Animals Blotting, Western Cell Line Electrophoretic Mobility Shift Assay Enzyme Activation Humans Immunohistochemistry Leukemia Inhibitory Factor Mammary Glands, Human Mice Mice, Inbred BALB C Mitogen-Activated Protein Kinase 1 Mitogen-Activated Protein Kinase 3 Transcription Factor AP-1 Tumor Necrosis Factor-alpha |
| description |
It has been reported that expression of tumor necrosis factor superfamily members occur at the onset of the mammary gland post-lactational involution. One of these proteins, tumor necrosis factor alpha (TNFα), is a major mediator of inflammation that is able to induce expression of several cytokines. Leukemia inhibitory factor (LIF) is an inflammatory cytokine that is induced and plays a fundamental role during postlactational involution of the mammary gland. Therefore, our goal was to determine whether TNFα activity in the mammary epithelium might include regulation of LIF expression. This biological role would increase the significance of TNFα expression at the end of lactation. Our results show that TNFα was able to induce LIF transcription through ERK1/2 activation in a non-tumorigenic mouse mammary epithelial cell line, SCp2. We found that activation of TNFα receptor-2 (TNFR2) was specifically involved in triggering this signaling pathway. In addition, our data suggest the participation of AP-1 transcription factor family members in this pathway. We determined that TNFα treatment induced c-fos transcription, and blocking AP-1 activity resulted in a significant inhibition of TNFα-induced LIF expression. Finally, we found that TNFα was also able to trigger LIF expression and ERK1/2 activation in the mouse mammary gland in vivo. Therefore, our data suggest that TNFα may contribute to mammary gland involution by, among other activities, eliciting LIF expression through ERK1/2 and AP1 activation. © 2010 Wiley-Liss, Inc. |
| format |
JOUR |
| author |
Levy, C.S. Slomiansky, V. Gattelli, A. Nahmod, K. Pelisch, F. Blaustein, M. Srebrow, A. Coso, O.A. Kordon, E.C. |
| author_facet |
Levy, C.S. Slomiansky, V. Gattelli, A. Nahmod, K. Pelisch, F. Blaustein, M. Srebrow, A. Coso, O.A. Kordon, E.C. |
| author_sort |
Levy, C.S. |
| title |
Tumor necrosis factor alpha induces LIF expression through ERK1/2 activation in mammary epithelial cells |
| title_short |
Tumor necrosis factor alpha induces LIF expression through ERK1/2 activation in mammary epithelial cells |
| title_full |
Tumor necrosis factor alpha induces LIF expression through ERK1/2 activation in mammary epithelial cells |
| title_fullStr |
Tumor necrosis factor alpha induces LIF expression through ERK1/2 activation in mammary epithelial cells |
| title_full_unstemmed |
Tumor necrosis factor alpha induces LIF expression through ERK1/2 activation in mammary epithelial cells |
| title_sort |
tumor necrosis factor alpha induces lif expression through erk1/2 activation in mammary epithelial cells |
| url |
http://hdl.handle.net/20.500.12110/paper_07302312_v110_n4_p857_Levy |
| work_keys_str_mv |
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| _version_ |
1807323361698643968 |