Analysis of the potato calcium-dependent protein kinase family and characterization of StCDPK7, a member induced upon infection with Phytophthora infestans

Key message: We describe the potato CDPK family and place StCDPK7 as a player in potato response to Phytophthora infestansinfection, identifying phenylalanine ammonia lyase as its specific phosphorylation target in vitro. Abstract: Calcium-dependent protein kinases (CDPKs) decode calcium (Ca2+) sign...

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Autores principales:	Fantino, E., Segretin, M.E., Santin, F., Mirkin, F.G., Ulloa, R.M.
Formato:	JOUR
Materias:	Calcium-dependent protein kinases Phenylalanine ammonia lyase Phytophthora infestans Solanum tuberosum calcium-dependent protein kinase phenylalanine ammonia lyase plant protein protein kinase cell nucleus cytosol disease resistance enzymology genetics metabolism microbiology parasitology physiology plant disease potato Cell Nucleus Cytosol Disease Resistance Phenylalanine Ammonia-Lyase Plant Diseases Plant Proteins Protein Kinases
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_07217714_v36_n7_p1137_Fantino
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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spelling	todo:paper_07217714_v36_n7_p1137_Fantino2023-10-03T15:36:51Z Analysis of the potato calcium-dependent protein kinase family and characterization of StCDPK7, a member induced upon infection with Phytophthora infestans Fantino, E. Segretin, M.E. Santin, F. Mirkin, F.G. Ulloa, R.M. Calcium-dependent protein kinases Phenylalanine ammonia lyase Phytophthora infestans Solanum tuberosum calcium-dependent protein kinase phenylalanine ammonia lyase plant protein protein kinase cell nucleus cytosol disease resistance enzymology genetics metabolism microbiology parasitology physiology Phytophthora infestans plant disease potato Cell Nucleus Cytosol Disease Resistance Phenylalanine Ammonia-Lyase Phytophthora infestans Plant Diseases Plant Proteins Protein Kinases Solanum tuberosum Key message: We describe the potato CDPK family and place StCDPK7 as a player in potato response to Phytophthora infestansinfection, identifying phenylalanine ammonia lyase as its specific phosphorylation target in vitro. Abstract: Calcium-dependent protein kinases (CDPKs) decode calcium (Ca2+) signals and activate different signaling pathways involved in hormone signaling, plant growth, development, and both abiotic and biotic stress responses. In this study, we describe the potato CDPK/CRK multigene family; bioinformatic analysis allowed us to identify 20 new CDPK isoforms, three CDPK-related kinases (CRKs), and a CDPK-like kinase. Phylogenetic analysis indicated that 26 StCDPKs can be classified into four groups, whose members are predicted to undergo different acylation patterns and exhibited diverse expression levels in different tissues and in response to various stimuli. With the aim of characterizing those members that are particularly involved in plant–pathogen interaction, we focused on StCDPK7. Tissue expression profile revealed that StCDPK7 transcript levels are high in swollen stolons, roots, and mini tubers. Moreover, its expression is induced upon Phytophthora infestans infection in systemic leaves. Transient expression assays showed that StCDPK7 displays a cytosolic/nuclear localization in spite of having a predicted chloroplast transit peptide. The recombinant protein, StCDPK7:6xHis, is an active Ca2+-dependent protein kinase that can phosphorylate phenylalanine ammonia lyase, an enzyme involved in plant defense response. The analysis of the potato CDPK family provides the first step towards the identification of CDPK isoforms involved in biotic stress. StCDPK7 emerges as a relevant player that could be manipulated to deploy disease resistance in potato crops. © 2017, Springer-Verlag Berlin Heidelberg. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_07217714_v36_n7_p1137_Fantino
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Calcium-dependent protein kinases Phenylalanine ammonia lyase Phytophthora infestans Solanum tuberosum calcium-dependent protein kinase phenylalanine ammonia lyase plant protein protein kinase cell nucleus cytosol disease resistance enzymology genetics metabolism microbiology parasitology physiology Phytophthora infestans plant disease potato Cell Nucleus Cytosol Disease Resistance Phenylalanine Ammonia-Lyase Phytophthora infestans Plant Diseases Plant Proteins Protein Kinases Solanum tuberosum
spellingShingle	Calcium-dependent protein kinases Phenylalanine ammonia lyase Phytophthora infestans Solanum tuberosum calcium-dependent protein kinase phenylalanine ammonia lyase plant protein protein kinase cell nucleus cytosol disease resistance enzymology genetics metabolism microbiology parasitology physiology Phytophthora infestans plant disease potato Cell Nucleus Cytosol Disease Resistance Phenylalanine Ammonia-Lyase Phytophthora infestans Plant Diseases Plant Proteins Protein Kinases Solanum tuberosum Fantino, E. Segretin, M.E. Santin, F. Mirkin, F.G. Ulloa, R.M. Analysis of the potato calcium-dependent protein kinase family and characterization of StCDPK7, a member induced upon infection with Phytophthora infestans
topic_facet	Calcium-dependent protein kinases Phenylalanine ammonia lyase Phytophthora infestans Solanum tuberosum calcium-dependent protein kinase phenylalanine ammonia lyase plant protein protein kinase cell nucleus cytosol disease resistance enzymology genetics metabolism microbiology parasitology physiology Phytophthora infestans plant disease potato Cell Nucleus Cytosol Disease Resistance Phenylalanine Ammonia-Lyase Phytophthora infestans Plant Diseases Plant Proteins Protein Kinases Solanum tuberosum
description	Key message: We describe the potato CDPK family and place StCDPK7 as a player in potato response to Phytophthora infestansinfection, identifying phenylalanine ammonia lyase as its specific phosphorylation target in vitro. Abstract: Calcium-dependent protein kinases (CDPKs) decode calcium (Ca2+) signals and activate different signaling pathways involved in hormone signaling, plant growth, development, and both abiotic and biotic stress responses. In this study, we describe the potato CDPK/CRK multigene family; bioinformatic analysis allowed us to identify 20 new CDPK isoforms, three CDPK-related kinases (CRKs), and a CDPK-like kinase. Phylogenetic analysis indicated that 26 StCDPKs can be classified into four groups, whose members are predicted to undergo different acylation patterns and exhibited diverse expression levels in different tissues and in response to various stimuli. With the aim of characterizing those members that are particularly involved in plant–pathogen interaction, we focused on StCDPK7. Tissue expression profile revealed that StCDPK7 transcript levels are high in swollen stolons, roots, and mini tubers. Moreover, its expression is induced upon Phytophthora infestans infection in systemic leaves. Transient expression assays showed that StCDPK7 displays a cytosolic/nuclear localization in spite of having a predicted chloroplast transit peptide. The recombinant protein, StCDPK7:6xHis, is an active Ca2+-dependent protein kinase that can phosphorylate phenylalanine ammonia lyase, an enzyme involved in plant defense response. The analysis of the potato CDPK family provides the first step towards the identification of CDPK isoforms involved in biotic stress. StCDPK7 emerges as a relevant player that could be manipulated to deploy disease resistance in potato crops. © 2017, Springer-Verlag Berlin Heidelberg.
format	JOUR
author	Fantino, E. Segretin, M.E. Santin, F. Mirkin, F.G. Ulloa, R.M.
author_facet	Fantino, E. Segretin, M.E. Santin, F. Mirkin, F.G. Ulloa, R.M.
author_sort	Fantino, E.
title	Analysis of the potato calcium-dependent protein kinase family and characterization of StCDPK7, a member induced upon infection with Phytophthora infestans
title_short	Analysis of the potato calcium-dependent protein kinase family and characterization of StCDPK7, a member induced upon infection with Phytophthora infestans
title_full	Analysis of the potato calcium-dependent protein kinase family and characterization of StCDPK7, a member induced upon infection with Phytophthora infestans
title_fullStr	Analysis of the potato calcium-dependent protein kinase family and characterization of StCDPK7, a member induced upon infection with Phytophthora infestans
title_full_unstemmed	Analysis of the potato calcium-dependent protein kinase family and characterization of StCDPK7, a member induced upon infection with Phytophthora infestans
title_sort	analysis of the potato calcium-dependent protein kinase family and characterization of stcdpk7, a member induced upon infection with phytophthora infestans
url	http://hdl.handle.net/20.500.12110/paper_07217714_v36_n7_p1137_Fantino
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Analysis of the potato calcium-dependent protein kinase family and characterization of StCDPK7, a member induced upon infection with Phytophthora infestans

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