Brassinosteroid-driven enhancement of the in vitro multiplication rate for the marubakaido apple rootstock [Malus prunifolia (Willd.) Borkh]

Treatment of shoot apices of the marubakaido apple rootstock with a fluoro derivative of 28-homoethylcastasterone (5F-HCTS) in the range 100-10,000 ng per shoot increased the apple rootstock's multiplication rate (MR). A dose of 500 ng 5F-HCTS in 5 μl ethanol, per shoot, resulted in a significa...

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Detalles Bibliográficos
Autores principales: Schaefer, S., Medeiro, S., Ramírez, J., Galagovsky, L., Pereira-Netto, A.
Formato: JOUR
Materias:
28-Homoethylcastasterone
Brassinosteroid derivative
Shoot proliferation
Ethanol
Fluorine compounds
Plant cell culture
Rootstocks
Plants (botany)
Malus
Malus prunifolia
Malus x domestica
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_07217714_v20_n11_p1093_Schaefer
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
Descripción
Sumario:Treatment of shoot apices of the marubakaido apple rootstock with a fluoro derivative of 28-homoethylcastasterone (5F-HCTS) in the range 100-10,000 ng per shoot increased the apple rootstock's multiplication rate (MR). A dose of 500 ng 5F-HCTS in 5 μl ethanol, per shoot, resulted in a significant (P=0.05) 112% increase in MR compared to shoots treated with 5 μl of 95% ethanol when the shoots were grown in culture medium enriched with 2.2 μM N6-benzyladenine. This increase in MR was due to: (1) a 15% increase in the number of main branches; (2) a 238% increase in the number of primary lateral branches; (3) a 250% increase in the number of secondary lateral branches. These results show that shoot proliferation induced by the fluoro derivative of 28-homocastasterone is an effective method to enhance the in vitro multiplication rate in Malus prunifolia.

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