Brassinosteroid-driven enhancement of the in vitro multiplication rate for the marubakaido apple rootstock [Malus prunifolia (Willd.) Borkh]

Treatment of shoot apices of the marubakaido apple rootstock with a fluoro derivative of 28-homoethylcastasterone (5F-HCTS) in the range 100-10,000 ng per shoot increased the apple rootstock's multiplication rate (MR). A dose of 500 ng 5F-HCTS in 5 μl ethanol, per shoot, resulted in a significa...

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Autores principales: Schaefer, S., Medeiro, S., Ramírez, J., Galagovsky, L., Pereira-Netto, A.
Formato: JOUR
Materias:
28-Homoethylcastasterone
Brassinosteroid derivative
Shoot proliferation
Ethanol
Fluorine compounds
Plant cell culture
Rootstocks
Plants (botany)
Malus
Malus prunifolia
Malus x domestica
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_07217714_v20_n11_p1093_Schaefer
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_07217714_v20_n11_p1093_Schaefer
record_format dspace
spelling todo:paper_07217714_v20_n11_p1093_Schaefer2023-10-03T15:36:49Z Brassinosteroid-driven enhancement of the in vitro multiplication rate for the marubakaido apple rootstock [Malus prunifolia (Willd.) Borkh] Schaefer, S. Medeiro, S. Ramírez, J. Galagovsky, L. Pereira-Netto, A. 28-Homoethylcastasterone Brassinosteroid derivative Shoot proliferation Ethanol Fluorine compounds Plant cell culture Rootstocks Plants (botany) Malus Malus prunifolia Malus x domestica Treatment of shoot apices of the marubakaido apple rootstock with a fluoro derivative of 28-homoethylcastasterone (5F-HCTS) in the range 100-10,000 ng per shoot increased the apple rootstock's multiplication rate (MR). A dose of 500 ng 5F-HCTS in 5 μl ethanol, per shoot, resulted in a significant (P=0.05) 112% increase in MR compared to shoots treated with 5 μl of 95% ethanol when the shoots were grown in culture medium enriched with 2.2 μM N6-benzyladenine. This increase in MR was due to: (1) a 15% increase in the number of main branches; (2) a 238% increase in the number of primary lateral branches; (3) a 250% increase in the number of secondary lateral branches. These results show that shoot proliferation induced by the fluoro derivative of 28-homocastasterone is an effective method to enhance the in vitro multiplication rate in Malus prunifolia. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_07217714_v20_n11_p1093_Schaefer
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic 28-Homoethylcastasterone
Brassinosteroid derivative
Shoot proliferation
Ethanol
Fluorine compounds
Plant cell culture
Rootstocks
Plants (botany)
Malus
Malus prunifolia
Malus x domestica
spellingShingle 28-Homoethylcastasterone
Brassinosteroid derivative
Shoot proliferation
Ethanol
Fluorine compounds
Plant cell culture
Rootstocks
Plants (botany)
Malus
Malus prunifolia
Malus x domestica
Schaefer, S.
Medeiro, S.
Ramírez, J.
Galagovsky, L.
Pereira-Netto, A.
Brassinosteroid-driven enhancement of the in vitro multiplication rate for the marubakaido apple rootstock [Malus prunifolia (Willd.) Borkh]
topic_facet 28-Homoethylcastasterone
Brassinosteroid derivative
Shoot proliferation
Ethanol
Fluorine compounds
Plant cell culture
Rootstocks
Plants (botany)
Malus
Malus prunifolia
Malus x domestica
description Treatment of shoot apices of the marubakaido apple rootstock with a fluoro derivative of 28-homoethylcastasterone (5F-HCTS) in the range 100-10,000 ng per shoot increased the apple rootstock's multiplication rate (MR). A dose of 500 ng 5F-HCTS in 5 μl ethanol, per shoot, resulted in a significant (P=0.05) 112% increase in MR compared to shoots treated with 5 μl of 95% ethanol when the shoots were grown in culture medium enriched with 2.2 μM N6-benzyladenine. This increase in MR was due to: (1) a 15% increase in the number of main branches; (2) a 238% increase in the number of primary lateral branches; (3) a 250% increase in the number of secondary lateral branches. These results show that shoot proliferation induced by the fluoro derivative of 28-homocastasterone is an effective method to enhance the in vitro multiplication rate in Malus prunifolia.
format JOUR
author Schaefer, S.
Medeiro, S.
Ramírez, J.
Galagovsky, L.
Pereira-Netto, A.
author_facet Schaefer, S.
Medeiro, S.
Ramírez, J.
Galagovsky, L.
Pereira-Netto, A.
author_sort Schaefer, S.
title Brassinosteroid-driven enhancement of the in vitro multiplication rate for the marubakaido apple rootstock [Malus prunifolia (Willd.) Borkh]
title_short Brassinosteroid-driven enhancement of the in vitro multiplication rate for the marubakaido apple rootstock [Malus prunifolia (Willd.) Borkh]
title_full Brassinosteroid-driven enhancement of the in vitro multiplication rate for the marubakaido apple rootstock [Malus prunifolia (Willd.) Borkh]
title_fullStr Brassinosteroid-driven enhancement of the in vitro multiplication rate for the marubakaido apple rootstock [Malus prunifolia (Willd.) Borkh]
title_full_unstemmed Brassinosteroid-driven enhancement of the in vitro multiplication rate for the marubakaido apple rootstock [Malus prunifolia (Willd.) Borkh]
title_sort brassinosteroid-driven enhancement of the in vitro multiplication rate for the marubakaido apple rootstock [malus prunifolia (willd.) borkh]
url http://hdl.handle.net/20.500.12110/paper_07217714_v20_n11_p1093_Schaefer
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AT medeiros brassinosteroiddrivenenhancementoftheinvitromultiplicationrateforthemarubakaidoapplerootstockmalusprunifoliawilldborkh
AT ramirezj brassinosteroiddrivenenhancementoftheinvitromultiplicationrateforthemarubakaidoapplerootstockmalusprunifoliawilldborkh
AT galagovskyl brassinosteroiddrivenenhancementoftheinvitromultiplicationrateforthemarubakaidoapplerootstockmalusprunifoliawilldborkh
AT pereiranettoa brassinosteroiddrivenenhancementoftheinvitromultiplicationrateforthemarubakaidoapplerootstockmalusprunifoliawilldborkh
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