A laboratory scale device for microencapsulation of genetically engineered cells into alginate beads

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The microencapsulation of recombinant cells, widely used for in vitro high-density cell culture, is a novel and potentially cost-effective method of in vivo heterologous protein delivery, where the protein producing cells are immunologically protected from tissue rejection. We report here a simple,...

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Detalles Bibliográficos
Autores principales: Fiszman, G.L., Karara, A.L., Finocchiaro, L.M.E., Glikin, G.C.
Formato: JOUR
Materias:
CHO cells
Interleukin-2
Protein delivery
calcium alginate
interleukin 2
animal cell
article
CHO cell
generator
microcapsule
microencapsulation
nonhuman
technique
Animalia
Cricetinae
Cricetulus griseus
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_07173458_v5_n3_p86_Fiszman
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_07173458_v5_n3_p86_Fiszman
record_format dspace
spelling todo:paper_07173458_v5_n3_p86_Fiszman2023-10-03T15:36:24Z A laboratory scale device for microencapsulation of genetically engineered cells into alginate beads Fiszman, G.L. Karara, A.L. Finocchiaro, L.M.E. Glikin, G.C. CHO cells Interleukin-2 Protein delivery calcium alginate interleukin 2 animal cell article CHO cell generator microcapsule microencapsulation nonhuman technique Animalia Cricetinae Cricetulus griseus The microencapsulation of recombinant cells, widely used for in vitro high-density cell culture, is a novel and potentially cost-effective method of in vivo heterologous protein delivery, where the protein producing cells are immunologically protected from tissue rejection. We report here a simple, reliable and inexpensive laboratory method to generate calcium alginate microcapsules containing genetically engineered, interleukin-2 expressing, Chinese hamster ovary (CHO) cells. Fil:Finocchiaro, L.M.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Glikin, G.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_07173458_v5_n3_p86_Fiszman
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic CHO cells
Interleukin-2
Protein delivery
calcium alginate
interleukin 2
animal cell
article
CHO cell
generator
microcapsule
microencapsulation
nonhuman
technique
Animalia
Cricetinae
Cricetulus griseus
spellingShingle CHO cells
Interleukin-2
Protein delivery
calcium alginate
interleukin 2
animal cell
article
CHO cell
generator
microcapsule
microencapsulation
nonhuman
technique
Animalia
Cricetinae
Cricetulus griseus
Fiszman, G.L.
Karara, A.L.
Finocchiaro, L.M.E.
Glikin, G.C.
A laboratory scale device for microencapsulation of genetically engineered cells into alginate beads
topic_facet CHO cells
Interleukin-2
Protein delivery
calcium alginate
interleukin 2
animal cell
article
CHO cell
generator
microcapsule
microencapsulation
nonhuman
technique
Animalia
Cricetinae
Cricetulus griseus
description The microencapsulation of recombinant cells, widely used for in vitro high-density cell culture, is a novel and potentially cost-effective method of in vivo heterologous protein delivery, where the protein producing cells are immunologically protected from tissue rejection. We report here a simple, reliable and inexpensive laboratory method to generate calcium alginate microcapsules containing genetically engineered, interleukin-2 expressing, Chinese hamster ovary (CHO) cells.
format JOUR
author Fiszman, G.L.
Karara, A.L.
Finocchiaro, L.M.E.
Glikin, G.C.
author_facet Fiszman, G.L.
Karara, A.L.
Finocchiaro, L.M.E.
Glikin, G.C.
author_sort Fiszman, G.L.
title A laboratory scale device for microencapsulation of genetically engineered cells into alginate beads
title_short A laboratory scale device for microencapsulation of genetically engineered cells into alginate beads
title_full A laboratory scale device for microencapsulation of genetically engineered cells into alginate beads
title_fullStr A laboratory scale device for microencapsulation of genetically engineered cells into alginate beads
title_full_unstemmed A laboratory scale device for microencapsulation of genetically engineered cells into alginate beads
title_sort laboratory scale device for microencapsulation of genetically engineered cells into alginate beads
url http://hdl.handle.net/20.500.12110/paper_07173458_v5_n3_p86_Fiszman
work_keys_str_mv AT fiszmangl alaboratoryscaledeviceformicroencapsulationofgeneticallyengineeredcellsintoalginatebeads
AT kararaal alaboratoryscaledeviceformicroencapsulationofgeneticallyengineeredcellsintoalginatebeads
AT finocchiarolme alaboratoryscaledeviceformicroencapsulationofgeneticallyengineeredcellsintoalginatebeads
AT glikingc alaboratoryscaledeviceformicroencapsulationofgeneticallyengineeredcellsintoalginatebeads
AT fiszmangl laboratoryscaledeviceformicroencapsulationofgeneticallyengineeredcellsintoalginatebeads
AT kararaal laboratoryscaledeviceformicroencapsulationofgeneticallyengineeredcellsintoalginatebeads
AT finocchiarolme laboratoryscaledeviceformicroencapsulationofgeneticallyengineeredcellsintoalginatebeads
AT glikingc laboratoryscaledeviceformicroencapsulationofgeneticallyengineeredcellsintoalginatebeads
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