Genetic mapping of EST-SSRs, SSR and InDels to improve saturation of genomic regions in a previously developed sunflower map

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In order to saturate a sunflower genetic map and facilitate marker-assisted selection (MAS) breeding for stress response, it is necessary to enhance map saturation with molecular markers localized in linkage groups associated to genomic regions involved in these traits. This work describes the ident...

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Autores principales: Talia, P., Nishinakamasu, V., Hopp, H.E., Heinz, R.A., Paniego, N.
Formato: JOUR
Materias:
EST-SSR
InDels
Linkage map
SSR
Sunflower
Plants (botany)
Genes
agronomic trait
amplified fragment length polymorphism
article
controlled study
expressed sequence tag
gene deletion
gene insertion
gene locus
gene mapping
genetic database
genetic linkage
genetic recombination
genetic trait
inbred strain
linkage analysis
nonhuman
nucleotide sequence
plant gene
polymerase chain reaction
simple sequence repeat
sunflower
Helianthus
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_07173458_v13_n6_p_Talia
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_07173458_v13_n6_p_Talia
record_format dspace
spelling todo:paper_07173458_v13_n6_p_Talia2023-10-03T15:36:22Z Genetic mapping of EST-SSRs, SSR and InDels to improve saturation of genomic regions in a previously developed sunflower map Talia, P. Nishinakamasu, V. Hopp, H.E. Heinz, R.A. Paniego, N. EST-SSR InDels Linkage map SSR Sunflower EST-SSR InDels Linkage map SSR Sunflower Plants (botany) Genes agronomic trait amplified fragment length polymorphism article controlled study expressed sequence tag gene deletion gene insertion gene locus gene mapping genetic database genetic linkage genetic recombination genetic trait inbred strain linkage analysis nonhuman nucleotide sequence plant gene polymerase chain reaction simple sequence repeat sunflower Helianthus In order to saturate a sunflower genetic map and facilitate marker-assisted selection (MAS) breeding for stress response, it is necessary to enhance map saturation with molecular markers localized in linkage groups associated to genomic regions involved in these traits. This work describes the identification and characterization of 1,134 simple sequence repeat (SSR) containing expressed sequence tags (ESTs) from unigenes available databases. Twelve of these functional markers as well as 41 public SSR markers were successfully localized in linkage groups, thus contributing to the saturation of specific regions on a reference genetic-linkage-map derived from recombinant inbred lines (RIL) mapping population from the cross between PAC2 x RHA266 lines. The enriched map includes 547 markers (231 SSR, 9 EST-SSR, 3 insertions/deletions (InDels) and 304 amplified fragment length polymorphisms (AFLPs) distributed in 17 linkage groups (LG), spanning genetic size to 1,942.3 cM and improving its mean density to 3.6 cM per locus. As consequence, no gaps longer than 13.2 cM remain uncovered throughout the entire map, which increases the feasibility of detecting genes or traits of agronomic importance in sunflower. © 2010 by Pontificia Universidad Católica de Valparaíso, Chile. Fil:Talia, P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Hopp, H.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Heinz, R.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_07173458_v13_n6_p_Talia
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic EST-SSR
InDels
Linkage map
SSR
Sunflower
EST-SSR
InDels
Linkage map
SSR
Sunflower
Plants (botany)
Genes
agronomic trait
amplified fragment length polymorphism
article
controlled study
expressed sequence tag
gene deletion
gene insertion
gene locus
gene mapping
genetic database
genetic linkage
genetic recombination
genetic trait
inbred strain
linkage analysis
nonhuman
nucleotide sequence
plant gene
polymerase chain reaction
simple sequence repeat
sunflower
Helianthus
spellingShingle EST-SSR
InDels
Linkage map
SSR
Sunflower
EST-SSR
InDels
Linkage map
SSR
Sunflower
Plants (botany)
Genes
agronomic trait
amplified fragment length polymorphism
article
controlled study
expressed sequence tag
gene deletion
gene insertion
gene locus
gene mapping
genetic database
genetic linkage
genetic recombination
genetic trait
inbred strain
linkage analysis
nonhuman
nucleotide sequence
plant gene
polymerase chain reaction
simple sequence repeat
sunflower
Helianthus
Talia, P.
Nishinakamasu, V.
Hopp, H.E.
Heinz, R.A.
Paniego, N.
Genetic mapping of EST-SSRs, SSR and InDels to improve saturation of genomic regions in a previously developed sunflower map
topic_facet EST-SSR
InDels
Linkage map
SSR
Sunflower
EST-SSR
InDels
Linkage map
SSR
Sunflower
Plants (botany)
Genes
agronomic trait
amplified fragment length polymorphism
article
controlled study
expressed sequence tag
gene deletion
gene insertion
gene locus
gene mapping
genetic database
genetic linkage
genetic recombination
genetic trait
inbred strain
linkage analysis
nonhuman
nucleotide sequence
plant gene
polymerase chain reaction
simple sequence repeat
sunflower
Helianthus
description In order to saturate a sunflower genetic map and facilitate marker-assisted selection (MAS) breeding for stress response, it is necessary to enhance map saturation with molecular markers localized in linkage groups associated to genomic regions involved in these traits. This work describes the identification and characterization of 1,134 simple sequence repeat (SSR) containing expressed sequence tags (ESTs) from unigenes available databases. Twelve of these functional markers as well as 41 public SSR markers were successfully localized in linkage groups, thus contributing to the saturation of specific regions on a reference genetic-linkage-map derived from recombinant inbred lines (RIL) mapping population from the cross between PAC2 x RHA266 lines. The enriched map includes 547 markers (231 SSR, 9 EST-SSR, 3 insertions/deletions (InDels) and 304 amplified fragment length polymorphisms (AFLPs) distributed in 17 linkage groups (LG), spanning genetic size to 1,942.3 cM and improving its mean density to 3.6 cM per locus. As consequence, no gaps longer than 13.2 cM remain uncovered throughout the entire map, which increases the feasibility of detecting genes or traits of agronomic importance in sunflower. © 2010 by Pontificia Universidad Católica de Valparaíso, Chile.
format JOUR
author Talia, P.
Nishinakamasu, V.
Hopp, H.E.
Heinz, R.A.
Paniego, N.
author_facet Talia, P.
Nishinakamasu, V.
Hopp, H.E.
Heinz, R.A.
Paniego, N.
author_sort Talia, P.
title Genetic mapping of EST-SSRs, SSR and InDels to improve saturation of genomic regions in a previously developed sunflower map
title_short Genetic mapping of EST-SSRs, SSR and InDels to improve saturation of genomic regions in a previously developed sunflower map
title_full Genetic mapping of EST-SSRs, SSR and InDels to improve saturation of genomic regions in a previously developed sunflower map
title_fullStr Genetic mapping of EST-SSRs, SSR and InDels to improve saturation of genomic regions in a previously developed sunflower map
title_full_unstemmed Genetic mapping of EST-SSRs, SSR and InDels to improve saturation of genomic regions in a previously developed sunflower map
title_sort genetic mapping of est-ssrs, ssr and indels to improve saturation of genomic regions in a previously developed sunflower map
url http://hdl.handle.net/20.500.12110/paper_07173458_v13_n6_p_Talia
work_keys_str_mv AT taliap geneticmappingofestssrsssrandindelstoimprovesaturationofgenomicregionsinapreviouslydevelopedsunflowermap
AT nishinakamasuv geneticmappingofestssrsssrandindelstoimprovesaturationofgenomicregionsinapreviouslydevelopedsunflowermap
AT hopphe geneticmappingofestssrsssrandindelstoimprovesaturationofgenomicregionsinapreviouslydevelopedsunflowermap
AT heinzra geneticmappingofestssrsssrandindelstoimprovesaturationofgenomicregionsinapreviouslydevelopedsunflowermap
AT paniegon geneticmappingofestssrsssrandindelstoimprovesaturationofgenomicregionsinapreviouslydevelopedsunflowermap
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