An α(D)-globin fragment from Triatoma infestans hindgut stimulates Trypanosoma cruzi adenylyl cyclase and promotes metacyclogenesis
A peptide from hindguts of the Triatoma infestans, the hematophagous Chagus' insect vector, activates adenylyl cyclase activity in Trypanosoma cruzi epimastigote membranes and stimulates the in vitro differentiation of epimastigotes (proliferative and non-infectious forms) to metacyclic trypoma...
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_07169760_v26_n1-2_p279_Fraidenraich |
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todo:paper_07169760_v26_n1-2_p279_Fraidenraich2023-10-03T15:36:18Z An α(D)-globin fragment from Triatoma infestans hindgut stimulates Trypanosoma cruzi adenylyl cyclase and promotes metacyclogenesis Fraidenraich, D. Pena, C. Isola, E.L. Lammel, E.M. Coso, O. Diaz Anel, A. Baralle, F. Torres, H.N. Flawia, M.M. adenylate cyclase alpha globin peptide animal tissue conference paper enzyme activation epimastigote insect intestine nonhuman trypanosoma cruzi Adenylate Cyclase Amino Acid Sequence Animal Chickens Digestive Physiology Globins Mice Molecular Sequence Data Peptide Fragments Support, Non-U.S. Gov't Triatoma Trypanosoma cruzi A peptide from hindguts of the Triatoma infestans, the hematophagous Chagus' insect vector, activates adenylyl cyclase activity in Trypanosoma cruzi epimastigote membranes and stimulates the in vitro differentiation of epimastigotes (proliferative and non-infectious forms) to metacyclic trypomastigotes (non-proliferative and infectious forms). The peptide was purified from hindguts of insects fed two days before with chicken blood. After purification, the peptide showed upon SDS-PAGE a single band of about 10 kDa. The sequence for 20 residues of the amino terminus of this peptide was: H2N-Met-Leu-Thr-Ala-Glu-Asp-Lys-Lys-Leu-Ile-Gln-Gln-Ala-Trp-Glu-Lys-A la-Ala-Ser-His. This sequence corresponds to the amino terminus of chicken α(D)-globin. A synthetic peptide with the sequence of the 40 amino acids corresponding to the amino terminus of α(D)-globin, also stimulated T. cruzi adenylyl cyclase activity and promoted metacyclogenesis. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_07169760_v26_n1-2_p279_Fraidenraich |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
adenylate cyclase alpha globin peptide animal tissue conference paper enzyme activation epimastigote insect intestine nonhuman trypanosoma cruzi Adenylate Cyclase Amino Acid Sequence Animal Chickens Digestive Physiology Globins Mice Molecular Sequence Data Peptide Fragments Support, Non-U.S. Gov't Triatoma Trypanosoma cruzi |
| spellingShingle |
adenylate cyclase alpha globin peptide animal tissue conference paper enzyme activation epimastigote insect intestine nonhuman trypanosoma cruzi Adenylate Cyclase Amino Acid Sequence Animal Chickens Digestive Physiology Globins Mice Molecular Sequence Data Peptide Fragments Support, Non-U.S. Gov't Triatoma Trypanosoma cruzi Fraidenraich, D. Pena, C. Isola, E.L. Lammel, E.M. Coso, O. Diaz Anel, A. Baralle, F. Torres, H.N. Flawia, M.M. An α(D)-globin fragment from Triatoma infestans hindgut stimulates Trypanosoma cruzi adenylyl cyclase and promotes metacyclogenesis |
| topic_facet |
adenylate cyclase alpha globin peptide animal tissue conference paper enzyme activation epimastigote insect intestine nonhuman trypanosoma cruzi Adenylate Cyclase Amino Acid Sequence Animal Chickens Digestive Physiology Globins Mice Molecular Sequence Data Peptide Fragments Support, Non-U.S. Gov't Triatoma Trypanosoma cruzi |
| description |
A peptide from hindguts of the Triatoma infestans, the hematophagous Chagus' insect vector, activates adenylyl cyclase activity in Trypanosoma cruzi epimastigote membranes and stimulates the in vitro differentiation of epimastigotes (proliferative and non-infectious forms) to metacyclic trypomastigotes (non-proliferative and infectious forms). The peptide was purified from hindguts of insects fed two days before with chicken blood. After purification, the peptide showed upon SDS-PAGE a single band of about 10 kDa. The sequence for 20 residues of the amino terminus of this peptide was: H2N-Met-Leu-Thr-Ala-Glu-Asp-Lys-Lys-Leu-Ile-Gln-Gln-Ala-Trp-Glu-Lys-A la-Ala-Ser-His. This sequence corresponds to the amino terminus of chicken α(D)-globin. A synthetic peptide with the sequence of the 40 amino acids corresponding to the amino terminus of α(D)-globin, also stimulated T. cruzi adenylyl cyclase activity and promoted metacyclogenesis. |
| format |
JOUR |
| author |
Fraidenraich, D. Pena, C. Isola, E.L. Lammel, E.M. Coso, O. Diaz Anel, A. Baralle, F. Torres, H.N. Flawia, M.M. |
| author_facet |
Fraidenraich, D. Pena, C. Isola, E.L. Lammel, E.M. Coso, O. Diaz Anel, A. Baralle, F. Torres, H.N. Flawia, M.M. |
| author_sort |
Fraidenraich, D. |
| title |
An α(D)-globin fragment from Triatoma infestans hindgut stimulates Trypanosoma cruzi adenylyl cyclase and promotes metacyclogenesis |
| title_short |
An α(D)-globin fragment from Triatoma infestans hindgut stimulates Trypanosoma cruzi adenylyl cyclase and promotes metacyclogenesis |
| title_full |
An α(D)-globin fragment from Triatoma infestans hindgut stimulates Trypanosoma cruzi adenylyl cyclase and promotes metacyclogenesis |
| title_fullStr |
An α(D)-globin fragment from Triatoma infestans hindgut stimulates Trypanosoma cruzi adenylyl cyclase and promotes metacyclogenesis |
| title_full_unstemmed |
An α(D)-globin fragment from Triatoma infestans hindgut stimulates Trypanosoma cruzi adenylyl cyclase and promotes metacyclogenesis |
| title_sort |
α(d)-globin fragment from triatoma infestans hindgut stimulates trypanosoma cruzi adenylyl cyclase and promotes metacyclogenesis |
| url |
http://hdl.handle.net/20.500.12110/paper_07169760_v26_n1-2_p279_Fraidenraich |
| work_keys_str_mv |
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