Preparation and Biological Activities of Sulfated Derivatives of (1→3)-β-D-Glucans
Sulfated derivatives of (1→3)-β-D-glucans with different degree of branching (DB) i.e., curdlan (linear, DB 0), grifolan (6-O-substituted by β-D-glucose at every third residue of the main chain, DB 1/3), and SSG (6-O-substituted by β-D-glucose at every second residue, DB 1/2) having DS value (degree...
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todo:paper_0386846X_v14_n5_p256_Suzuki2023-10-03T15:33:44Z Preparation and Biological Activities of Sulfated Derivatives of (1→3)-β-D-Glucans Suzuki, T. Ohno, N. Adachi, Y. Cirelli, A.F. Covian, J.A. Yadomae, T. (1→3)-β-D-glucan biological activity grifolan SSG sulfated derivative beta 1,3 glucan curdlan grifolan immunomodulating agent animal cell article blood clotting complement system concentration response controlled study drug synthesis human human tissue lymphocyte proliferation macrophage activation male mitogenesis mouse nonhuman sulfation Animal Blood Coagulation Chemistry, Physical Complement Pathway, Alternative Glucans Human In Vitro Macrophage Activation Male Mice Mice, Inbred C3H Mice, Inbred ICR Mitogens Molecular Weight Sulfated derivatives of (1→3)-β-D-glucans with different degree of branching (DB) i.e., curdlan (linear, DB 0), grifolan (6-O-substituted by β-D-glucose at every third residue of the main chain, DB 1/3), and SSG (6-O-substituted by β-D-glucose at every second residue, DB 1/2) having DS value (degree of substitution) lower than 0.6 were prepared. Biological activities [clotting of plasma, alternative pathway of complement (APC), proliferative response of murine spleen cells (mitogenic activity), and activation of murine peritoneal macrophages in vitro] of these derivatives were examined. Clotting of plasma was inhibited by the derivatives having DS above 0.2. APC was inhibited by incubation with derivatives and was strongly dependent on substitution. Inhibition of APC was the most significant in sulfated SSG having DS 0.6 [SSG(3)]. Mitogenic activity was observed by most of the derivatives and the highest activity was shown by SSG(l) (DS 0.2). Macrophage was also activated but SSG(l) would lose the capability to recognize the receptor for (1 →3)-β-D-glucans. From these results, it appears that the biological activities of (1→3)-β-D-glucans were significantly modulated by sulfation and the effect was dependent on both DS and DB. © 1991, The Pharmaceutical Society of Japan. All rights reserved. Fil:Covian, J.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_0386846X_v14_n5_p256_Suzuki |
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Universidad de Buenos Aires |
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I-28 |
| repository_str |
R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
(1→3)-β-D-glucan biological activity grifolan SSG sulfated derivative beta 1,3 glucan curdlan grifolan immunomodulating agent animal cell article blood clotting complement system concentration response controlled study drug synthesis human human tissue lymphocyte proliferation macrophage activation male mitogenesis mouse nonhuman sulfation Animal Blood Coagulation Chemistry, Physical Complement Pathway, Alternative Glucans Human In Vitro Macrophage Activation Male Mice Mice, Inbred C3H Mice, Inbred ICR Mitogens Molecular Weight |
| spellingShingle |
(1→3)-β-D-glucan biological activity grifolan SSG sulfated derivative beta 1,3 glucan curdlan grifolan immunomodulating agent animal cell article blood clotting complement system concentration response controlled study drug synthesis human human tissue lymphocyte proliferation macrophage activation male mitogenesis mouse nonhuman sulfation Animal Blood Coagulation Chemistry, Physical Complement Pathway, Alternative Glucans Human In Vitro Macrophage Activation Male Mice Mice, Inbred C3H Mice, Inbred ICR Mitogens Molecular Weight Suzuki, T. Ohno, N. Adachi, Y. Cirelli, A.F. Covian, J.A. Yadomae, T. Preparation and Biological Activities of Sulfated Derivatives of (1→3)-β-D-Glucans |
| topic_facet |
(1→3)-β-D-glucan biological activity grifolan SSG sulfated derivative beta 1,3 glucan curdlan grifolan immunomodulating agent animal cell article blood clotting complement system concentration response controlled study drug synthesis human human tissue lymphocyte proliferation macrophage activation male mitogenesis mouse nonhuman sulfation Animal Blood Coagulation Chemistry, Physical Complement Pathway, Alternative Glucans Human In Vitro Macrophage Activation Male Mice Mice, Inbred C3H Mice, Inbred ICR Mitogens Molecular Weight |
| description |
Sulfated derivatives of (1→3)-β-D-glucans with different degree of branching (DB) i.e., curdlan (linear, DB 0), grifolan (6-O-substituted by β-D-glucose at every third residue of the main chain, DB 1/3), and SSG (6-O-substituted by β-D-glucose at every second residue, DB 1/2) having DS value (degree of substitution) lower than 0.6 were prepared. Biological activities [clotting of plasma, alternative pathway of complement (APC), proliferative response of murine spleen cells (mitogenic activity), and activation of murine peritoneal macrophages in vitro] of these derivatives were examined. Clotting of plasma was inhibited by the derivatives having DS above 0.2. APC was inhibited by incubation with derivatives and was strongly dependent on substitution. Inhibition of APC was the most significant in sulfated SSG having DS 0.6 [SSG(3)]. Mitogenic activity was observed by most of the derivatives and the highest activity was shown by SSG(l) (DS 0.2). Macrophage was also activated but SSG(l) would lose the capability to recognize the receptor for (1 →3)-β-D-glucans. From these results, it appears that the biological activities of (1→3)-β-D-glucans were significantly modulated by sulfation and the effect was dependent on both DS and DB. © 1991, The Pharmaceutical Society of Japan. All rights reserved. |
| format |
JOUR |
| author |
Suzuki, T. Ohno, N. Adachi, Y. Cirelli, A.F. Covian, J.A. Yadomae, T. |
| author_facet |
Suzuki, T. Ohno, N. Adachi, Y. Cirelli, A.F. Covian, J.A. Yadomae, T. |
| author_sort |
Suzuki, T. |
| title |
Preparation and Biological Activities of Sulfated Derivatives of (1→3)-β-D-Glucans |
| title_short |
Preparation and Biological Activities of Sulfated Derivatives of (1→3)-β-D-Glucans |
| title_full |
Preparation and Biological Activities of Sulfated Derivatives of (1→3)-β-D-Glucans |
| title_fullStr |
Preparation and Biological Activities of Sulfated Derivatives of (1→3)-β-D-Glucans |
| title_full_unstemmed |
Preparation and Biological Activities of Sulfated Derivatives of (1→3)-β-D-Glucans |
| title_sort |
preparation and biological activities of sulfated derivatives of (1→3)-β-d-glucans |
| url |
http://hdl.handle.net/20.500.12110/paper_0386846X_v14_n5_p256_Suzuki |
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