DNA single strand breaks in peripheral blood lymphocytes induced by three nitroimidazole derivatives
Tinidazole (TNZ), ornidazole (ONZ) and metronidazole (MTZ) are antiparasitic drugs (nitroimidazole derivatives) that have proven to be effective against Trichomonas vaginalis, Entoamoeba histolytica, Giardia lamblia and Helicobacter pylori. The reduction of the nitro group and the generation of shor...
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todo:paper_03784274_v132_n2_p109_RodriguezFerreiro2023-10-03T15:32:19Z DNA single strand breaks in peripheral blood lymphocytes induced by three nitroimidazole derivatives Rodriguez Ferreiro, G. Cancino Badóas, L. Lopez-Nigro, M. Palermo, A. Mudry, M. González Elio, P. Carballo, M.A. Comet assay DNA damage Metronidazole Nitroimidazoles Ornidazole Tinidazole DNA metronidazole nitroimidazole derivative ornidazole reactive oxygen metabolite tinidazole aerobic metabolism antimicrobial activity article bacterial cell comet assay concentration response controlled study DNA damage DNA strand breakage drug cytotoxicity human human cell lymphocyte mammal cell mutagenic activity priority journal Adult Antitrichomonal Agents Cells, Cultured Comet Assay DNA Damage DNA, Single-Stranded Dose-Response Relationship, Drug Humans Lymphocytes Male Metronidazole Nitroimidazoles Ornidazole Tinidazole Giardia Giardia intestinalis Helicobacter Helicobacter pylori Mammalia Trichomonas vaginalis Tinidazole (TNZ), ornidazole (ONZ) and metronidazole (MTZ) are antiparasitic drugs (nitroimidazole derivatives) that have proven to be effective against Trichomonas vaginalis, Entoamoeba histolytica, Giardia lamblia and Helicobacter pylori. The reduction of the nitro group and the generation of short-lived reactive intermediates are the basis of its parasiticidal activity. This reduction is associated with its mutagenic activity in bacteria, although in mammalian cells DNA damage seems to be related to the production of reactive oxygen species (ROS). Using alkaline single cell electrophoresis, a significant increase in single strand breaks and alkali labile sites in human peripheral blood lymphocytes (PBL) exposed to MTZ, ONZ and TNZ at 10, 100 and 500 μg/ml is observed. MTZ causes less damage, especially at higher concentrations, when compared with TNZ, the most harmful of the drugs tested. These findings suggest that primary damage is induced under aerobic conditions and confirms that these nitroimidazoles are DNA damaging agents. © 2002 Elsevier Science Ireland Ltd. All rights reserved. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_03784274_v132_n2_p109_RodriguezFerreiro |
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Universidad de Buenos Aires |
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I-28 |
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R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Comet assay DNA damage Metronidazole Nitroimidazoles Ornidazole Tinidazole DNA metronidazole nitroimidazole derivative ornidazole reactive oxygen metabolite tinidazole aerobic metabolism antimicrobial activity article bacterial cell comet assay concentration response controlled study DNA damage DNA strand breakage drug cytotoxicity human human cell lymphocyte mammal cell mutagenic activity priority journal Adult Antitrichomonal Agents Cells, Cultured Comet Assay DNA Damage DNA, Single-Stranded Dose-Response Relationship, Drug Humans Lymphocytes Male Metronidazole Nitroimidazoles Ornidazole Tinidazole Giardia Giardia intestinalis Helicobacter Helicobacter pylori Mammalia Trichomonas vaginalis |
| spellingShingle |
Comet assay DNA damage Metronidazole Nitroimidazoles Ornidazole Tinidazole DNA metronidazole nitroimidazole derivative ornidazole reactive oxygen metabolite tinidazole aerobic metabolism antimicrobial activity article bacterial cell comet assay concentration response controlled study DNA damage DNA strand breakage drug cytotoxicity human human cell lymphocyte mammal cell mutagenic activity priority journal Adult Antitrichomonal Agents Cells, Cultured Comet Assay DNA Damage DNA, Single-Stranded Dose-Response Relationship, Drug Humans Lymphocytes Male Metronidazole Nitroimidazoles Ornidazole Tinidazole Giardia Giardia intestinalis Helicobacter Helicobacter pylori Mammalia Trichomonas vaginalis Rodriguez Ferreiro, G. Cancino Badóas, L. Lopez-Nigro, M. Palermo, A. Mudry, M. González Elio, P. Carballo, M.A. DNA single strand breaks in peripheral blood lymphocytes induced by three nitroimidazole derivatives |
| topic_facet |
Comet assay DNA damage Metronidazole Nitroimidazoles Ornidazole Tinidazole DNA metronidazole nitroimidazole derivative ornidazole reactive oxygen metabolite tinidazole aerobic metabolism antimicrobial activity article bacterial cell comet assay concentration response controlled study DNA damage DNA strand breakage drug cytotoxicity human human cell lymphocyte mammal cell mutagenic activity priority journal Adult Antitrichomonal Agents Cells, Cultured Comet Assay DNA Damage DNA, Single-Stranded Dose-Response Relationship, Drug Humans Lymphocytes Male Metronidazole Nitroimidazoles Ornidazole Tinidazole Giardia Giardia intestinalis Helicobacter Helicobacter pylori Mammalia Trichomonas vaginalis |
| description |
Tinidazole (TNZ), ornidazole (ONZ) and metronidazole (MTZ) are antiparasitic drugs (nitroimidazole derivatives) that have proven to be effective against Trichomonas vaginalis, Entoamoeba histolytica, Giardia lamblia and Helicobacter pylori. The reduction of the nitro group and the generation of short-lived reactive intermediates are the basis of its parasiticidal activity. This reduction is associated with its mutagenic activity in bacteria, although in mammalian cells DNA damage seems to be related to the production of reactive oxygen species (ROS). Using alkaline single cell electrophoresis, a significant increase in single strand breaks and alkali labile sites in human peripheral blood lymphocytes (PBL) exposed to MTZ, ONZ and TNZ at 10, 100 and 500 μg/ml is observed. MTZ causes less damage, especially at higher concentrations, when compared with TNZ, the most harmful of the drugs tested. These findings suggest that primary damage is induced under aerobic conditions and confirms that these nitroimidazoles are DNA damaging agents. © 2002 Elsevier Science Ireland Ltd. All rights reserved. |
| format |
JOUR |
| author |
Rodriguez Ferreiro, G. Cancino Badóas, L. Lopez-Nigro, M. Palermo, A. Mudry, M. González Elio, P. Carballo, M.A. |
| author_facet |
Rodriguez Ferreiro, G. Cancino Badóas, L. Lopez-Nigro, M. Palermo, A. Mudry, M. González Elio, P. Carballo, M.A. |
| author_sort |
Rodriguez Ferreiro, G. |
| title |
DNA single strand breaks in peripheral blood lymphocytes induced by three nitroimidazole derivatives |
| title_short |
DNA single strand breaks in peripheral blood lymphocytes induced by three nitroimidazole derivatives |
| title_full |
DNA single strand breaks in peripheral blood lymphocytes induced by three nitroimidazole derivatives |
| title_fullStr |
DNA single strand breaks in peripheral blood lymphocytes induced by three nitroimidazole derivatives |
| title_full_unstemmed |
DNA single strand breaks in peripheral blood lymphocytes induced by three nitroimidazole derivatives |
| title_sort |
dna single strand breaks in peripheral blood lymphocytes induced by three nitroimidazole derivatives |
| url |
http://hdl.handle.net/20.500.12110/paper_03784274_v132_n2_p109_RodriguezFerreiro |
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