Nuclear DNA content in the polyploid complex Turnera ulmifolia (Turnera L., Passifloraceae)

Turnera ulmifolia constitutes a well-studied polyploid complex with allo- and autopolyploid species ranging from 2 to 8x. Flow cytometry was used to determine nuclear DNA content, and to estimate 2C- and 1Cx-values with the aim of analysing the genome size in Turnera in terms of polyploid speciation...

Descripción completa

Detalles Bibliográficos
Autores principales: López, A., Panseri, A.F., Poggio, L., Fernández, A.
Formato: JOUR
Materias:
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_03782697_v296_n3-4_p225_Lopez
Aporte de:
id todo:paper_03782697_v296_n3-4_p225_Lopez
record_format dspace
spelling todo:paper_03782697_v296_n3-4_p225_Lopez2023-10-03T15:32:10Z Nuclear DNA content in the polyploid complex Turnera ulmifolia (Turnera L., Passifloraceae) López, A. Panseri, A.F. Poggio, L. Fernández, A. 2C-value Cx-value DNA content Passifloraceae Polyploid complex Turnera Turneraceae Passiflora edulis Passifloraceae Turnera Turnera aurelii Turnera cuneiformis Turnera grandidentata Turnera ulmifolia Turneraceae Turnera ulmifolia constitutes a well-studied polyploid complex with allo- and autopolyploid species ranging from 2 to 8x. Flow cytometry was used to determine nuclear DNA content, and to estimate 2C- and 1Cx-values with the aim of analysing the genome size in Turnera in terms of polyploid speciation. The 2C-value and 1Cx-value were evaluated in 12 species of the T. ulmifolia complex. Nuclear DNA content was estimated by flow cytometry of nuclei stained with propidium iodide. The 2C DNA content ranged from 1.38 to 1.83 pg in diploids, from 2.67 to 3.96 pg in tetraploids, from 2.73 to 4.31 pg in hexaploids, and from 3.53 to 5.90 pg in octoploids. The 1Cx-value ranged from 0.44 to 0.99 pg. The Turnera ulmifolia complex showed an increase in total DNA content in the ploidy level, but not in the expected proportion. The general tendency indicated a decrease in the 1Cx-value with increasing chromosome number, with T. grandidentata 4x being an outstanding exception. The 1Cx-values in the allooctoploids T. aurelii and T. cuneiformis differed by 1.6-fold from each other, probably as a result of different evolutionary histories following divergence from the last common ancestor. © 2011 Springer-Verlag. Fil:López, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Poggio, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_03782697_v296_n3-4_p225_Lopez
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic 2C-value
Cx-value
DNA content
Passifloraceae
Polyploid complex
Turnera
Turneraceae
Passiflora edulis
Passifloraceae
Turnera
Turnera aurelii
Turnera cuneiformis
Turnera grandidentata
Turnera ulmifolia
Turneraceae
spellingShingle 2C-value
Cx-value
DNA content
Passifloraceae
Polyploid complex
Turnera
Turneraceae
Passiflora edulis
Passifloraceae
Turnera
Turnera aurelii
Turnera cuneiformis
Turnera grandidentata
Turnera ulmifolia
Turneraceae
López, A.
Panseri, A.F.
Poggio, L.
Fernández, A.
Nuclear DNA content in the polyploid complex Turnera ulmifolia (Turnera L., Passifloraceae)
topic_facet 2C-value
Cx-value
DNA content
Passifloraceae
Polyploid complex
Turnera
Turneraceae
Passiflora edulis
Passifloraceae
Turnera
Turnera aurelii
Turnera cuneiformis
Turnera grandidentata
Turnera ulmifolia
Turneraceae
description Turnera ulmifolia constitutes a well-studied polyploid complex with allo- and autopolyploid species ranging from 2 to 8x. Flow cytometry was used to determine nuclear DNA content, and to estimate 2C- and 1Cx-values with the aim of analysing the genome size in Turnera in terms of polyploid speciation. The 2C-value and 1Cx-value were evaluated in 12 species of the T. ulmifolia complex. Nuclear DNA content was estimated by flow cytometry of nuclei stained with propidium iodide. The 2C DNA content ranged from 1.38 to 1.83 pg in diploids, from 2.67 to 3.96 pg in tetraploids, from 2.73 to 4.31 pg in hexaploids, and from 3.53 to 5.90 pg in octoploids. The 1Cx-value ranged from 0.44 to 0.99 pg. The Turnera ulmifolia complex showed an increase in total DNA content in the ploidy level, but not in the expected proportion. The general tendency indicated a decrease in the 1Cx-value with increasing chromosome number, with T. grandidentata 4x being an outstanding exception. The 1Cx-values in the allooctoploids T. aurelii and T. cuneiformis differed by 1.6-fold from each other, probably as a result of different evolutionary histories following divergence from the last common ancestor. © 2011 Springer-Verlag.
format JOUR
author López, A.
Panseri, A.F.
Poggio, L.
Fernández, A.
author_facet López, A.
Panseri, A.F.
Poggio, L.
Fernández, A.
author_sort López, A.
title Nuclear DNA content in the polyploid complex Turnera ulmifolia (Turnera L., Passifloraceae)
title_short Nuclear DNA content in the polyploid complex Turnera ulmifolia (Turnera L., Passifloraceae)
title_full Nuclear DNA content in the polyploid complex Turnera ulmifolia (Turnera L., Passifloraceae)
title_fullStr Nuclear DNA content in the polyploid complex Turnera ulmifolia (Turnera L., Passifloraceae)
title_full_unstemmed Nuclear DNA content in the polyploid complex Turnera ulmifolia (Turnera L., Passifloraceae)
title_sort nuclear dna content in the polyploid complex turnera ulmifolia (turnera l., passifloraceae)
url http://hdl.handle.net/20.500.12110/paper_03782697_v296_n3-4_p225_Lopez
work_keys_str_mv AT lopeza nucleardnacontentinthepolyploidcomplexturneraulmifoliaturneralpassifloraceae
AT panseriaf nucleardnacontentinthepolyploidcomplexturneraulmifoliaturneralpassifloraceae
AT poggiol nucleardnacontentinthepolyploidcomplexturneraulmifoliaturneralpassifloraceae
AT fernandeza nucleardnacontentinthepolyploidcomplexturneraulmifoliaturneralpassifloraceae
_version_ 1807320725007106048