Sequence evolution of the major satellite DNA of the genus Ctenomys (Octodontidae, Rodentia)
Sequence variability of RPCS (repetitive PuvII Ctenomys sequence), the major satellite DNA of octodontid Ctenomys rodents, was analysed in species belonging to three groups of species representing the two patterns of karyotypic evolution in the genus: stable and dynamic karyotypes among closely rela...
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todo:paper_03781119_v392_n1-2_p283_Ellingsen2023-10-03T15:31:50Z Sequence evolution of the major satellite DNA of the genus Ctenomys (Octodontidae, Rodentia) Ellingsen, A. Slamovits, C.H. Rossi, M.S. Concerted evolution Ctenomys Satellite DNA satellite DNA animal tissue article cladogenesis controlled study correlation analysis DNA sequence gene amplification gene number gene replication genetic stability genome karyotyping molecular cloning nonhuman nucleotide sequence polymerase chain reaction priority journal rodent Amino Acid Sequence Animals Base Sequence Deoxyribonucleases, Type II Site-Specific DNA, Satellite Evolution, Molecular Gene Amplification Gene Library Molecular Sequence Data Phylogeny Rodentia Sequence Homology Ctenomys Octodontidae Rodentia Sequence variability of RPCS (repetitive PuvII Ctenomys sequence), the major satellite DNA of octodontid Ctenomys rodents, was analysed in species belonging to three groups of species representing the two patterns of karyotypic evolution in the genus: stable and dynamic karyotypes among closely related species. The studied species represent the overall range of RPCS copy number (2000-6.6 × 106 copies per haploid genome) in the genus. RPCS sequence was characterised by PCR amplification of the genomic consensus sequence and cloned monomers. Our results suggest that RPCS genomic consensus sequence variability correlates with RPCS copy number stability and karyotypic stastis, but not with high or low RPCS copy number values. In contrast, the RPCS gcs shows a mutational profile that is similar across all analysed species. Our data suggest that an RPCS ancestral library of variants was maintained through the cladogenesis of the genus. There is also evidence pointing to the simultaneous contribution of processes of concerted evolution that resulted in a reduced representation of some ancestral variants and their partial replacement for new ones. In addition, analysis of distribution of the variability along the monomer suggests that subsequences of the RPCS are subject to some degree of constraint, probably driven by the recent replicative activity of RPCS in species with high copy number. © 2007 Elsevier B.V. All rights reserved. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_03781119_v392_n1-2_p283_Ellingsen |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Concerted evolution Ctenomys Satellite DNA satellite DNA animal tissue article cladogenesis controlled study correlation analysis DNA sequence gene amplification gene number gene replication genetic stability genome karyotyping molecular cloning nonhuman nucleotide sequence polymerase chain reaction priority journal rodent Amino Acid Sequence Animals Base Sequence Deoxyribonucleases, Type II Site-Specific DNA, Satellite Evolution, Molecular Gene Amplification Gene Library Molecular Sequence Data Phylogeny Rodentia Sequence Homology Ctenomys Octodontidae Rodentia |
| spellingShingle |
Concerted evolution Ctenomys Satellite DNA satellite DNA animal tissue article cladogenesis controlled study correlation analysis DNA sequence gene amplification gene number gene replication genetic stability genome karyotyping molecular cloning nonhuman nucleotide sequence polymerase chain reaction priority journal rodent Amino Acid Sequence Animals Base Sequence Deoxyribonucleases, Type II Site-Specific DNA, Satellite Evolution, Molecular Gene Amplification Gene Library Molecular Sequence Data Phylogeny Rodentia Sequence Homology Ctenomys Octodontidae Rodentia Ellingsen, A. Slamovits, C.H. Rossi, M.S. Sequence evolution of the major satellite DNA of the genus Ctenomys (Octodontidae, Rodentia) |
| topic_facet |
Concerted evolution Ctenomys Satellite DNA satellite DNA animal tissue article cladogenesis controlled study correlation analysis DNA sequence gene amplification gene number gene replication genetic stability genome karyotyping molecular cloning nonhuman nucleotide sequence polymerase chain reaction priority journal rodent Amino Acid Sequence Animals Base Sequence Deoxyribonucleases, Type II Site-Specific DNA, Satellite Evolution, Molecular Gene Amplification Gene Library Molecular Sequence Data Phylogeny Rodentia Sequence Homology Ctenomys Octodontidae Rodentia |
| description |
Sequence variability of RPCS (repetitive PuvII Ctenomys sequence), the major satellite DNA of octodontid Ctenomys rodents, was analysed in species belonging to three groups of species representing the two patterns of karyotypic evolution in the genus: stable and dynamic karyotypes among closely related species. The studied species represent the overall range of RPCS copy number (2000-6.6 × 106 copies per haploid genome) in the genus. RPCS sequence was characterised by PCR amplification of the genomic consensus sequence and cloned monomers. Our results suggest that RPCS genomic consensus sequence variability correlates with RPCS copy number stability and karyotypic stastis, but not with high or low RPCS copy number values. In contrast, the RPCS gcs shows a mutational profile that is similar across all analysed species. Our data suggest that an RPCS ancestral library of variants was maintained through the cladogenesis of the genus. There is also evidence pointing to the simultaneous contribution of processes of concerted evolution that resulted in a reduced representation of some ancestral variants and their partial replacement for new ones. In addition, analysis of distribution of the variability along the monomer suggests that subsequences of the RPCS are subject to some degree of constraint, probably driven by the recent replicative activity of RPCS in species with high copy number. © 2007 Elsevier B.V. All rights reserved. |
| format |
JOUR |
| author |
Ellingsen, A. Slamovits, C.H. Rossi, M.S. |
| author_facet |
Ellingsen, A. Slamovits, C.H. Rossi, M.S. |
| author_sort |
Ellingsen, A. |
| title |
Sequence evolution of the major satellite DNA of the genus Ctenomys (Octodontidae, Rodentia) |
| title_short |
Sequence evolution of the major satellite DNA of the genus Ctenomys (Octodontidae, Rodentia) |
| title_full |
Sequence evolution of the major satellite DNA of the genus Ctenomys (Octodontidae, Rodentia) |
| title_fullStr |
Sequence evolution of the major satellite DNA of the genus Ctenomys (Octodontidae, Rodentia) |
| title_full_unstemmed |
Sequence evolution of the major satellite DNA of the genus Ctenomys (Octodontidae, Rodentia) |
| title_sort |
sequence evolution of the major satellite dna of the genus ctenomys (octodontidae, rodentia) |
| url |
http://hdl.handle.net/20.500.12110/paper_03781119_v392_n1-2_p283_Ellingsen |
| work_keys_str_mv |
AT ellingsena sequenceevolutionofthemajorsatellitednaofthegenusctenomysoctodontidaerodentia AT slamovitsch sequenceevolutionofthemajorsatellitednaofthegenusctenomysoctodontidaerodentia AT rossims sequenceevolutionofthemajorsatellitednaofthegenusctenomysoctodontidaerodentia |
| _version_ |
1782027091614629888 |