Gene expression patterns in Euglena gracilis: Insights into the cellular response to environmental stress
To better understand Euglena gracilis gene expression under different stress conditions (Chromium, Streptomycin or darkness), we undertook a survey of the E. gracilis transcriptome by cDNA sequencing and microarray analysis. First, we constructed a non-normalized cDNA library from the E. gracilis UT...
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todo:paper_03781119_v389_n2_p136_dosSantosFerreira2023-10-03T15:31:50Z Gene expression patterns in Euglena gracilis: Insights into the cellular response to environmental stress dos Santos Ferreira, V. Rocchetta, I. Conforti, V. Bench, S. Feldman, R. Levin, M.J. cDNA library Chromium EST Microarray Streptomycin chromium complementary DNA streptomycin article DNA microarray DNA sequence environmental stress Euglena gracilis expressed sequence tag gene expression nonhuman nucleotide sequence priority journal Animals Chromium Darkness DNA, Algal DNA, Complementary DNA, Protozoan Euglena gracilis Expressed Sequence Tags Gene Expression Profiling Gene Expression Regulation Gene Library Genes, Protozoan Streptomycin Euglena gracilis Plantae To better understand Euglena gracilis gene expression under different stress conditions (Chromium, Streptomycin or darkness), we undertook a survey of the E. gracilis transcriptome by cDNA sequencing and microarray analysis. First, we constructed a non-normalized cDNA library from the E. gracilis UTEX strain and sequenced a total of 1000 cDNAs. Six hundred and ten of these ESTs were similar to either Plantae or Protistae genes (e-value < e- 10). Second, microarrays were built by spotting all the ESTs onto mirror slides. Microarray expression analysis indicated that 90 out of those 610 ESTs changed their expression level in response to different stress treatments (p < 0.05). In addition, we detected 10 ESTs that changed expression levels irrespective of the tested stress. These may be considered as part of a larger set of stress-related genes in E. gracilis. Finally, we identified 23 unknown ESTs (U-ESTs) following the expression profiles of these putative stress-related genes suggesting that they could be related to the cellular mechanism of stress response. © 2006 Elsevier B.V. All rights reserved. Fil:Rocchetta, I. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Conforti, V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_03781119_v389_n2_p136_dosSantosFerreira |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
cDNA library Chromium EST Microarray Streptomycin chromium complementary DNA streptomycin article DNA microarray DNA sequence environmental stress Euglena gracilis expressed sequence tag gene expression nonhuman nucleotide sequence priority journal Animals Chromium Darkness DNA, Algal DNA, Complementary DNA, Protozoan Euglena gracilis Expressed Sequence Tags Gene Expression Profiling Gene Expression Regulation Gene Library Genes, Protozoan Streptomycin Euglena gracilis Plantae |
| spellingShingle |
cDNA library Chromium EST Microarray Streptomycin chromium complementary DNA streptomycin article DNA microarray DNA sequence environmental stress Euglena gracilis expressed sequence tag gene expression nonhuman nucleotide sequence priority journal Animals Chromium Darkness DNA, Algal DNA, Complementary DNA, Protozoan Euglena gracilis Expressed Sequence Tags Gene Expression Profiling Gene Expression Regulation Gene Library Genes, Protozoan Streptomycin Euglena gracilis Plantae dos Santos Ferreira, V. Rocchetta, I. Conforti, V. Bench, S. Feldman, R. Levin, M.J. Gene expression patterns in Euglena gracilis: Insights into the cellular response to environmental stress |
| topic_facet |
cDNA library Chromium EST Microarray Streptomycin chromium complementary DNA streptomycin article DNA microarray DNA sequence environmental stress Euglena gracilis expressed sequence tag gene expression nonhuman nucleotide sequence priority journal Animals Chromium Darkness DNA, Algal DNA, Complementary DNA, Protozoan Euglena gracilis Expressed Sequence Tags Gene Expression Profiling Gene Expression Regulation Gene Library Genes, Protozoan Streptomycin Euglena gracilis Plantae |
| description |
To better understand Euglena gracilis gene expression under different stress conditions (Chromium, Streptomycin or darkness), we undertook a survey of the E. gracilis transcriptome by cDNA sequencing and microarray analysis. First, we constructed a non-normalized cDNA library from the E. gracilis UTEX strain and sequenced a total of 1000 cDNAs. Six hundred and ten of these ESTs were similar to either Plantae or Protistae genes (e-value < e- 10). Second, microarrays were built by spotting all the ESTs onto mirror slides. Microarray expression analysis indicated that 90 out of those 610 ESTs changed their expression level in response to different stress treatments (p < 0.05). In addition, we detected 10 ESTs that changed expression levels irrespective of the tested stress. These may be considered as part of a larger set of stress-related genes in E. gracilis. Finally, we identified 23 unknown ESTs (U-ESTs) following the expression profiles of these putative stress-related genes suggesting that they could be related to the cellular mechanism of stress response. © 2006 Elsevier B.V. All rights reserved. |
| format |
JOUR |
| author |
dos Santos Ferreira, V. Rocchetta, I. Conforti, V. Bench, S. Feldman, R. Levin, M.J. |
| author_facet |
dos Santos Ferreira, V. Rocchetta, I. Conforti, V. Bench, S. Feldman, R. Levin, M.J. |
| author_sort |
dos Santos Ferreira, V. |
| title |
Gene expression patterns in Euglena gracilis: Insights into the cellular response to environmental stress |
| title_short |
Gene expression patterns in Euglena gracilis: Insights into the cellular response to environmental stress |
| title_full |
Gene expression patterns in Euglena gracilis: Insights into the cellular response to environmental stress |
| title_fullStr |
Gene expression patterns in Euglena gracilis: Insights into the cellular response to environmental stress |
| title_full_unstemmed |
Gene expression patterns in Euglena gracilis: Insights into the cellular response to environmental stress |
| title_sort |
gene expression patterns in euglena gracilis: insights into the cellular response to environmental stress |
| url |
http://hdl.handle.net/20.500.12110/paper_03781119_v389_n2_p136_dosSantosFerreira |
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