Normal induction of the SOS response in Bacillus subtilis is prevented by the mutant repressor from phage φ105cts23
The presence of the Φ105cts23 mutant prophage in Bacillus subtilis induces a series of pleiotropic effects that could be ascribed to an anti-SOS activity. In order to circumvent the phage function responsible for this phenomenon, the cts23 mutant repressor was cloned and sequenced. The isolated repr...
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todo:paper_03781097_v167_n2_p315_Rubinstein2023-10-03T15:31:39Z Normal induction of the SOS response in Bacillus subtilis is prevented by the mutant repressor from phage φ105cts23 Rubinstein, C.P. Guerchicoff, A. Sanchez-Rivas, C. Bacillus subtilis Bacteriophage φ105 Repair SOS induction Thermosensitive repressor article bacillus subtilis bacteriophage dna repair heat sensitivity mutant nonhuman priority journal Artificial Gene Fusion Bacillus Phages Bacillus subtilis beta-Galactosidase DNA Damage DNA Repair Gene Expression Regulation, Bacterial Genes, Viral Mutation Plasmids Repressor Proteins SOS Response (Genetics) Temperature Viral Proteins Bacillus subtilis The presence of the Φ105cts23 mutant prophage in Bacillus subtilis induces a series of pleiotropic effects that could be ascribed to an anti-SOS activity. In order to circumvent the phage function responsible for this phenomenon, the cts23 mutant repressor was cloned and sequenced. The isolated repressor reduced the survival capacity of the host cells after mitomycin C or nalidixic acid treatments and lowered the spontaneous reversion frequency. When SOS induction kinetics were studied, low or null induction of the damage-inducible din22::LacZ fusion was observed. In contrast, the presence of the wild-type prophage amplified the SOS response. Sequencing of the mutant repressor revealed that the cts23 mutation is a T→C transition affecting the 5' closest codon to one of the two reported DNA binding domains. Copyright (C) 1998 Federation of European Microbiological Societies. Fil:Rubinstein, C.P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_03781097_v167_n2_p315_Rubinstein |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Bacillus subtilis Bacteriophage φ105 Repair SOS induction Thermosensitive repressor article bacillus subtilis bacteriophage dna repair heat sensitivity mutant nonhuman priority journal Artificial Gene Fusion Bacillus Phages Bacillus subtilis beta-Galactosidase DNA Damage DNA Repair Gene Expression Regulation, Bacterial Genes, Viral Mutation Plasmids Repressor Proteins SOS Response (Genetics) Temperature Viral Proteins Bacillus subtilis |
| spellingShingle |
Bacillus subtilis Bacteriophage φ105 Repair SOS induction Thermosensitive repressor article bacillus subtilis bacteriophage dna repair heat sensitivity mutant nonhuman priority journal Artificial Gene Fusion Bacillus Phages Bacillus subtilis beta-Galactosidase DNA Damage DNA Repair Gene Expression Regulation, Bacterial Genes, Viral Mutation Plasmids Repressor Proteins SOS Response (Genetics) Temperature Viral Proteins Bacillus subtilis Rubinstein, C.P. Guerchicoff, A. Sanchez-Rivas, C. Normal induction of the SOS response in Bacillus subtilis is prevented by the mutant repressor from phage φ105cts23 |
| topic_facet |
Bacillus subtilis Bacteriophage φ105 Repair SOS induction Thermosensitive repressor article bacillus subtilis bacteriophage dna repair heat sensitivity mutant nonhuman priority journal Artificial Gene Fusion Bacillus Phages Bacillus subtilis beta-Galactosidase DNA Damage DNA Repair Gene Expression Regulation, Bacterial Genes, Viral Mutation Plasmids Repressor Proteins SOS Response (Genetics) Temperature Viral Proteins Bacillus subtilis |
| description |
The presence of the Φ105cts23 mutant prophage in Bacillus subtilis induces a series of pleiotropic effects that could be ascribed to an anti-SOS activity. In order to circumvent the phage function responsible for this phenomenon, the cts23 mutant repressor was cloned and sequenced. The isolated repressor reduced the survival capacity of the host cells after mitomycin C or nalidixic acid treatments and lowered the spontaneous reversion frequency. When SOS induction kinetics were studied, low or null induction of the damage-inducible din22::LacZ fusion was observed. In contrast, the presence of the wild-type prophage amplified the SOS response. Sequencing of the mutant repressor revealed that the cts23 mutation is a T→C transition affecting the 5' closest codon to one of the two reported DNA binding domains. Copyright (C) 1998 Federation of European Microbiological Societies. |
| format |
JOUR |
| author |
Rubinstein, C.P. Guerchicoff, A. Sanchez-Rivas, C. |
| author_facet |
Rubinstein, C.P. Guerchicoff, A. Sanchez-Rivas, C. |
| author_sort |
Rubinstein, C.P. |
| title |
Normal induction of the SOS response in Bacillus subtilis is prevented by the mutant repressor from phage φ105cts23 |
| title_short |
Normal induction of the SOS response in Bacillus subtilis is prevented by the mutant repressor from phage φ105cts23 |
| title_full |
Normal induction of the SOS response in Bacillus subtilis is prevented by the mutant repressor from phage φ105cts23 |
| title_fullStr |
Normal induction of the SOS response in Bacillus subtilis is prevented by the mutant repressor from phage φ105cts23 |
| title_full_unstemmed |
Normal induction of the SOS response in Bacillus subtilis is prevented by the mutant repressor from phage φ105cts23 |
| title_sort |
normal induction of the sos response in bacillus subtilis is prevented by the mutant repressor from phage φ105cts23 |
| url |
http://hdl.handle.net/20.500.12110/paper_03781097_v167_n2_p315_Rubinstein |
| work_keys_str_mv |
AT rubinsteincp normalinductionofthesosresponseinbacillussubtilisispreventedbythemutantrepressorfromphageph105cts23 AT guerchicoffa normalinductionofthesosresponseinbacillussubtilisispreventedbythemutantrepressorfromphageph105cts23 AT sanchezrivasc normalinductionofthesosresponseinbacillussubtilisispreventedbythemutantrepressorfromphageph105cts23 |
| _version_ |
1782026940348104704 |