Mapping of B cell epitopes in an immunodominant antigen of Trypanosoma cruzi using fusions to the Escherichia coli LamB protein
The JL8 protein antigen from Trypanosoma cruzi, a dominant immunogen in man, has been characterized as containing tandem amino acid repeats. Here, we describe the use of the Lamb protein of Escherichia coli as a carrier of JL8 derived sequences in order to map the immunodominant B cell epitopes in t...
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_03781097_v164_n1_p125_Pereira |
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todo:paper_03781097_v164_n1_p125_Pereira2023-10-03T15:31:39Z Mapping of B cell epitopes in an immunodominant antigen of Trypanosoma cruzi using fusions to the Escherichia coli LamB protein Pereira, C.M. Yamauchi, L.M. Levin, M.J. Da Silveira, J.F. Castilho, B.A. B cell epitope mapping Chagas disease Escherichia coli LamB protein Immunodominant repetitive antigen Trypanosoma cruzi bacterial protein amino acid sequence antibody response antigen binding article chagas disease epitope mapping escherichia coli immune response nonhuman nucleotide sequence priority journal serodiagnosis trypanosoma cruzi Animals Antibodies, Bacterial Antibodies, Protozoan Antigens, Protozoan B-Lymphocytes Bacterial Proteins Cloning, Molecular Electrophoresis, Polyacrylamide Gel Enzyme-Linked Immunosorbent Assay Epitope Mapping Epitopes Escherichia coli Escherichia coli Proteins Humans Immunoblotting Immunodominant Epitopes Protein Sorting Signals Rabbits Recombinant Fusion Proteins Trypanosoma cruzi Bacteria (microorganisms) Escherichia coli Protozoa Trypanosoma Trypanosoma cruzi The JL8 protein antigen from Trypanosoma cruzi, a dominant immunogen in man, has been characterized as containing tandem amino acid repeats. Here, we describe the use of the Lamb protein of Escherichia coli as a carrier of JL8 derived sequences in order to map the immunodominant B cell epitopes in this antigen. Five different sequences of JL8 were inserted in the Lamb protein and the JL8-LamB fusion proteins were tested by ELISA with human chronic chagasic sera. The fusion carrying the sequence AEKQKAAEATKVAE was recognized by most sera. This protein was also capable of inhibiting the binding of human chagasic antibodies to GST-JL8 in competitive ELISA suggesting that it contains an immunodominant B cell epitope of JL8. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_03781097_v164_n1_p125_Pereira |
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Universidad de Buenos Aires |
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I-28 |
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R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
B cell epitope mapping Chagas disease Escherichia coli LamB protein Immunodominant repetitive antigen Trypanosoma cruzi bacterial protein amino acid sequence antibody response antigen binding article chagas disease epitope mapping escherichia coli immune response nonhuman nucleotide sequence priority journal serodiagnosis trypanosoma cruzi Animals Antibodies, Bacterial Antibodies, Protozoan Antigens, Protozoan B-Lymphocytes Bacterial Proteins Cloning, Molecular Electrophoresis, Polyacrylamide Gel Enzyme-Linked Immunosorbent Assay Epitope Mapping Epitopes Escherichia coli Escherichia coli Proteins Humans Immunoblotting Immunodominant Epitopes Protein Sorting Signals Rabbits Recombinant Fusion Proteins Trypanosoma cruzi Bacteria (microorganisms) Escherichia coli Protozoa Trypanosoma Trypanosoma cruzi |
| spellingShingle |
B cell epitope mapping Chagas disease Escherichia coli LamB protein Immunodominant repetitive antigen Trypanosoma cruzi bacterial protein amino acid sequence antibody response antigen binding article chagas disease epitope mapping escherichia coli immune response nonhuman nucleotide sequence priority journal serodiagnosis trypanosoma cruzi Animals Antibodies, Bacterial Antibodies, Protozoan Antigens, Protozoan B-Lymphocytes Bacterial Proteins Cloning, Molecular Electrophoresis, Polyacrylamide Gel Enzyme-Linked Immunosorbent Assay Epitope Mapping Epitopes Escherichia coli Escherichia coli Proteins Humans Immunoblotting Immunodominant Epitopes Protein Sorting Signals Rabbits Recombinant Fusion Proteins Trypanosoma cruzi Bacteria (microorganisms) Escherichia coli Protozoa Trypanosoma Trypanosoma cruzi Pereira, C.M. Yamauchi, L.M. Levin, M.J. Da Silveira, J.F. Castilho, B.A. Mapping of B cell epitopes in an immunodominant antigen of Trypanosoma cruzi using fusions to the Escherichia coli LamB protein |
| topic_facet |
B cell epitope mapping Chagas disease Escherichia coli LamB protein Immunodominant repetitive antigen Trypanosoma cruzi bacterial protein amino acid sequence antibody response antigen binding article chagas disease epitope mapping escherichia coli immune response nonhuman nucleotide sequence priority journal serodiagnosis trypanosoma cruzi Animals Antibodies, Bacterial Antibodies, Protozoan Antigens, Protozoan B-Lymphocytes Bacterial Proteins Cloning, Molecular Electrophoresis, Polyacrylamide Gel Enzyme-Linked Immunosorbent Assay Epitope Mapping Epitopes Escherichia coli Escherichia coli Proteins Humans Immunoblotting Immunodominant Epitopes Protein Sorting Signals Rabbits Recombinant Fusion Proteins Trypanosoma cruzi Bacteria (microorganisms) Escherichia coli Protozoa Trypanosoma Trypanosoma cruzi |
| description |
The JL8 protein antigen from Trypanosoma cruzi, a dominant immunogen in man, has been characterized as containing tandem amino acid repeats. Here, we describe the use of the Lamb protein of Escherichia coli as a carrier of JL8 derived sequences in order to map the immunodominant B cell epitopes in this antigen. Five different sequences of JL8 were inserted in the Lamb protein and the JL8-LamB fusion proteins were tested by ELISA with human chronic chagasic sera. The fusion carrying the sequence AEKQKAAEATKVAE was recognized by most sera. This protein was also capable of inhibiting the binding of human chagasic antibodies to GST-JL8 in competitive ELISA suggesting that it contains an immunodominant B cell epitope of JL8. |
| format |
JOUR |
| author |
Pereira, C.M. Yamauchi, L.M. Levin, M.J. Da Silveira, J.F. Castilho, B.A. |
| author_facet |
Pereira, C.M. Yamauchi, L.M. Levin, M.J. Da Silveira, J.F. Castilho, B.A. |
| author_sort |
Pereira, C.M. |
| title |
Mapping of B cell epitopes in an immunodominant antigen of Trypanosoma cruzi using fusions to the Escherichia coli LamB protein |
| title_short |
Mapping of B cell epitopes in an immunodominant antigen of Trypanosoma cruzi using fusions to the Escherichia coli LamB protein |
| title_full |
Mapping of B cell epitopes in an immunodominant antigen of Trypanosoma cruzi using fusions to the Escherichia coli LamB protein |
| title_fullStr |
Mapping of B cell epitopes in an immunodominant antigen of Trypanosoma cruzi using fusions to the Escherichia coli LamB protein |
| title_full_unstemmed |
Mapping of B cell epitopes in an immunodominant antigen of Trypanosoma cruzi using fusions to the Escherichia coli LamB protein |
| title_sort |
mapping of b cell epitopes in an immunodominant antigen of trypanosoma cruzi using fusions to the escherichia coli lamb protein |
| url |
http://hdl.handle.net/20.500.12110/paper_03781097_v164_n1_p125_Pereira |
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