The effects of oligosaccharide trimming inhibitors on glycoprotein expression and infectivity of Junin virus
The effects of specific inhibitors of glycoprotein trimming reactions on Junin virus (JV) replication were investigated. Bromoconduritol, an inhibitor of glucosidase II, significantly reduced infective virus production (DE50: 1.1 mM) and viral protein expression. Neither 1-deoxynojirimycin, an inhib...
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todo:paper_03781097_v109_n1_p39_Silber2023-10-03T15:31:38Z The effects of oligosaccharide trimming inhibitors on glycoprotein expression and infectivity of Junin virus Silber, A.M. Candurra, N.A. Elsa B., D. Glycoprotein expression Glycoprotein processing Junin virus Oligosaccharide trimming inhibitors Virus infectively 1 deoxymannonojirimycin 1 deoxynojirimycin bromoconduritol enzyme inhibitor glycoprotein glycosidase inhibitor oligosaccharide swainsonine animal cell article cell culture etiology glycoprotein synthesis junin virus monkey nonhuman priority journal protein processing virus infectivity virus replication 1-Deoxynojirimycin alpha-Glucosidases Animal Arenaviruses, New World Fluorescent Antibody Technique Glycoproteins Inositol Oligosaccharides Support, Non-U.S. Gov't Swainsonine Vero Cells Viral Proteins Virus Replication Animalia Junin virus The effects of specific inhibitors of glycoprotein trimming reactions on Junin virus (JV) replication were investigated. Bromoconduritol, an inhibitor of glucosidase II, significantly reduced infective virus production (DE50: 1.1 mM) and viral protein expression. Neither 1-deoxynojirimycin, an inhibitor of both glucosidases I and II, nor 1-deoxymannojirimycin and swainsonine, inhibitors of mannosidase I and II, respectively, showed any activity against JV multiplication. These results are the first evidence that the acquisition of a complex form of the envelope glycoprotein oligosaccharide chains is not essential for JV infectivity. The effect of bromoconduritol was reversible and probably due to the formation of an unstable intermediate oligosaccharide structure which may be more sensitive to degradative proteolysis. © 1993. Fil:Silber, A.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Candurra, N.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_03781097_v109_n1_p39_Silber |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Glycoprotein expression Glycoprotein processing Junin virus Oligosaccharide trimming inhibitors Virus infectively 1 deoxymannonojirimycin 1 deoxynojirimycin bromoconduritol enzyme inhibitor glycoprotein glycosidase inhibitor oligosaccharide swainsonine animal cell article cell culture etiology glycoprotein synthesis junin virus monkey nonhuman priority journal protein processing virus infectivity virus replication 1-Deoxynojirimycin alpha-Glucosidases Animal Arenaviruses, New World Fluorescent Antibody Technique Glycoproteins Inositol Oligosaccharides Support, Non-U.S. Gov't Swainsonine Vero Cells Viral Proteins Virus Replication Animalia Junin virus |
| spellingShingle |
Glycoprotein expression Glycoprotein processing Junin virus Oligosaccharide trimming inhibitors Virus infectively 1 deoxymannonojirimycin 1 deoxynojirimycin bromoconduritol enzyme inhibitor glycoprotein glycosidase inhibitor oligosaccharide swainsonine animal cell article cell culture etiology glycoprotein synthesis junin virus monkey nonhuman priority journal protein processing virus infectivity virus replication 1-Deoxynojirimycin alpha-Glucosidases Animal Arenaviruses, New World Fluorescent Antibody Technique Glycoproteins Inositol Oligosaccharides Support, Non-U.S. Gov't Swainsonine Vero Cells Viral Proteins Virus Replication Animalia Junin virus Silber, A.M. Candurra, N.A. Elsa B., D. The effects of oligosaccharide trimming inhibitors on glycoprotein expression and infectivity of Junin virus |
| topic_facet |
Glycoprotein expression Glycoprotein processing Junin virus Oligosaccharide trimming inhibitors Virus infectively 1 deoxymannonojirimycin 1 deoxynojirimycin bromoconduritol enzyme inhibitor glycoprotein glycosidase inhibitor oligosaccharide swainsonine animal cell article cell culture etiology glycoprotein synthesis junin virus monkey nonhuman priority journal protein processing virus infectivity virus replication 1-Deoxynojirimycin alpha-Glucosidases Animal Arenaviruses, New World Fluorescent Antibody Technique Glycoproteins Inositol Oligosaccharides Support, Non-U.S. Gov't Swainsonine Vero Cells Viral Proteins Virus Replication Animalia Junin virus |
| description |
The effects of specific inhibitors of glycoprotein trimming reactions on Junin virus (JV) replication were investigated. Bromoconduritol, an inhibitor of glucosidase II, significantly reduced infective virus production (DE50: 1.1 mM) and viral protein expression. Neither 1-deoxynojirimycin, an inhibitor of both glucosidases I and II, nor 1-deoxymannojirimycin and swainsonine, inhibitors of mannosidase I and II, respectively, showed any activity against JV multiplication. These results are the first evidence that the acquisition of a complex form of the envelope glycoprotein oligosaccharide chains is not essential for JV infectivity. The effect of bromoconduritol was reversible and probably due to the formation of an unstable intermediate oligosaccharide structure which may be more sensitive to degradative proteolysis. © 1993. |
| format |
JOUR |
| author |
Silber, A.M. Candurra, N.A. Elsa B., D. |
| author_facet |
Silber, A.M. Candurra, N.A. Elsa B., D. |
| author_sort |
Silber, A.M. |
| title |
The effects of oligosaccharide trimming inhibitors on glycoprotein expression and infectivity of Junin virus |
| title_short |
The effects of oligosaccharide trimming inhibitors on glycoprotein expression and infectivity of Junin virus |
| title_full |
The effects of oligosaccharide trimming inhibitors on glycoprotein expression and infectivity of Junin virus |
| title_fullStr |
The effects of oligosaccharide trimming inhibitors on glycoprotein expression and infectivity of Junin virus |
| title_full_unstemmed |
The effects of oligosaccharide trimming inhibitors on glycoprotein expression and infectivity of Junin virus |
| title_sort |
effects of oligosaccharide trimming inhibitors on glycoprotein expression and infectivity of junin virus |
| url |
http://hdl.handle.net/20.500.12110/paper_03781097_v109_n1_p39_Silber |
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