Depressed erythroid progenitor cell activity in aluminum-overloaded mice

The aim of the present study was to evaluate the effect of aluminum (A1) on in vivo erythropoiesis in mice that had received short- and long-term A1 overloading. At the end of each treatment period, clonal assays of-late erythroid progenitor cells (CFU-E) stimulated in vitro with erythropoietin were...

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Autores principales:	Garbossa, G., Gutnisky, A., Nesse, A.
Formato:	JOUR
Materias:	Aluminum Aluminum toxicity Anemia CFU-E Erythroid colony-forming units Erythropoiesis Median osmotic fragility Osmotic fragility curves aluminum endogenous compound erythropoietin hemoglobin animal cell animal model article bone controlled study erythroid precursor cell erythropoiesis hematocrit histology liver mouse nonhuman priority journal Aluminum Compounds Animals Bone and Bones Cell Division Chlorides Citric Acid Erythroid Progenitor Cells Erythropoietin Hematocrit Hemoglobins Liver Mice Mice, Inbred C3H Osmotic Fragility
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_03780392_v22_n4_p214_Garbossa
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_03780392_v22_n4_p214_Garbossa
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spelling	todo:paper_03780392_v22_n4_p214_Garbossa2023-10-03T15:31:37Z Depressed erythroid progenitor cell activity in aluminum-overloaded mice Garbossa, G. Gutnisky, A. Nesse, A. Aluminum Aluminum toxicity Anemia CFU-E Erythroid colony-forming units Erythropoiesis Median osmotic fragility Osmotic fragility curves aluminum endogenous compound erythropoietin hemoglobin animal cell animal model article bone controlled study erythroid precursor cell erythropoiesis hematocrit histology liver mouse nonhuman priority journal Aluminum Aluminum Compounds Animals Bone and Bones Cell Division Chlorides Citric Acid Erythroid Progenitor Cells Erythropoiesis Erythropoietin Hematocrit Hemoglobins Liver Mice Mice, Inbred C3H Osmotic Fragility The aim of the present study was to evaluate the effect of aluminum (A1) on in vivo erythropoiesis in mice that had received short- and long-term A1 overloading. At the end of each treatment period, clonal assays of-late erythroid progenitor cells (CFU-E) stimulated in vitro with erythropoietin were carried out, hematological parameters determined, and histological aluminon staining performed on bone and liver. After 2 weeks of-oral ingestion of either A1 chloride or A1 citrate (10 pmol per day), poor CFU-E growth was obtained but no differences in hematocrit (Ht) and hemoglobin (Hb) values were found when comparing the treated and control groups. CFU-E development, determined after loading mice with A1 citrate during 22 weeks (10 μmol/day), proved to be heavily depressed, and significant reductions in Ht, Hb concentration and erythrocyte osmotic fragility were also observed. No A1 accumulation could be demonstrated in tissue, using histological aluminon staining. The results suggest that, even in the absence of signs of anemia, ingested A1 may depress hematopoiesis by affecting red blood cell production and cell destruction. Fil:Garbossa, G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Nesse, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_03780392_v22_n4_p214_Garbossa
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Aluminum Aluminum toxicity Anemia CFU-E Erythroid colony-forming units Erythropoiesis Median osmotic fragility Osmotic fragility curves aluminum endogenous compound erythropoietin hemoglobin animal cell animal model article bone controlled study erythroid precursor cell erythropoiesis hematocrit histology liver mouse nonhuman priority journal Aluminum Aluminum Compounds Animals Bone and Bones Cell Division Chlorides Citric Acid Erythroid Progenitor Cells Erythropoiesis Erythropoietin Hematocrit Hemoglobins Liver Mice Mice, Inbred C3H Osmotic Fragility
spellingShingle	Aluminum Aluminum toxicity Anemia CFU-E Erythroid colony-forming units Erythropoiesis Median osmotic fragility Osmotic fragility curves aluminum endogenous compound erythropoietin hemoglobin animal cell animal model article bone controlled study erythroid precursor cell erythropoiesis hematocrit histology liver mouse nonhuman priority journal Aluminum Aluminum Compounds Animals Bone and Bones Cell Division Chlorides Citric Acid Erythroid Progenitor Cells Erythropoiesis Erythropoietin Hematocrit Hemoglobins Liver Mice Mice, Inbred C3H Osmotic Fragility Garbossa, G. Gutnisky, A. Nesse, A. Depressed erythroid progenitor cell activity in aluminum-overloaded mice
topic_facet	Aluminum Aluminum toxicity Anemia CFU-E Erythroid colony-forming units Erythropoiesis Median osmotic fragility Osmotic fragility curves aluminum endogenous compound erythropoietin hemoglobin animal cell animal model article bone controlled study erythroid precursor cell erythropoiesis hematocrit histology liver mouse nonhuman priority journal Aluminum Aluminum Compounds Animals Bone and Bones Cell Division Chlorides Citric Acid Erythroid Progenitor Cells Erythropoiesis Erythropoietin Hematocrit Hemoglobins Liver Mice Mice, Inbred C3H Osmotic Fragility
description	The aim of the present study was to evaluate the effect of aluminum (A1) on in vivo erythropoiesis in mice that had received short- and long-term A1 overloading. At the end of each treatment period, clonal assays of-late erythroid progenitor cells (CFU-E) stimulated in vitro with erythropoietin were carried out, hematological parameters determined, and histological aluminon staining performed on bone and liver. After 2 weeks of-oral ingestion of either A1 chloride or A1 citrate (10 pmol per day), poor CFU-E growth was obtained but no differences in hematocrit (Ht) and hemoglobin (Hb) values were found when comparing the treated and control groups. CFU-E development, determined after loading mice with A1 citrate during 22 weeks (10 μmol/day), proved to be heavily depressed, and significant reductions in Ht, Hb concentration and erythrocyte osmotic fragility were also observed. No A1 accumulation could be demonstrated in tissue, using histological aluminon staining. The results suggest that, even in the absence of signs of anemia, ingested A1 may depress hematopoiesis by affecting red blood cell production and cell destruction.
format	JOUR
author	Garbossa, G. Gutnisky, A. Nesse, A.
author_facet	Garbossa, G. Gutnisky, A. Nesse, A.
author_sort	Garbossa, G.
title	Depressed erythroid progenitor cell activity in aluminum-overloaded mice
title_short	Depressed erythroid progenitor cell activity in aluminum-overloaded mice
title_full	Depressed erythroid progenitor cell activity in aluminum-overloaded mice
title_fullStr	Depressed erythroid progenitor cell activity in aluminum-overloaded mice
title_full_unstemmed	Depressed erythroid progenitor cell activity in aluminum-overloaded mice
title_sort	depressed erythroid progenitor cell activity in aluminum-overloaded mice
url	http://hdl.handle.net/20.500.12110/paper_03780392_v22_n4_p214_Garbossa
work_keys_str_mv	AT garbossag depressederythroidprogenitorcellactivityinaluminumoverloadedmice AT gutniskya depressederythroidprogenitorcellactivityinaluminumoverloadedmice AT nessea depressederythroidprogenitorcellactivityinaluminumoverloadedmice
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Depressed erythroid progenitor cell activity in aluminum-overloaded mice

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