Identification of polydisperse RNA in the cytoplasm of neurons isolated from the immature brain cortex

The cytoplasmic RNA of 10-day-old rats was studied in bulk-isolated, cortical neurons, obtained under conditions which minimize nuclear contamination. Two RNA fractions, one enriched in rRNA and the other in "polydisperse" RNA, were obtained by differential extraction with phenol. Gel elec...

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Autores principales: Azcurra, J.M., Carrasco, A.E.
Formato: JOUR
Materias:
rna
animal experiment
brain cortex
central nervous system
nerve cell
nervous system
rat
Animal
Cerebral Cortex
Cytoplasm
Electrophoresis, Polyacrylamide Gel
Molecular Weight
Rats
RNA
RNA, Ribosomal
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_03643190_v3_n5_p521_Azcurra
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_03643190_v3_n5_p521_Azcurra
record_format dspace
spelling todo:paper_03643190_v3_n5_p521_Azcurra2023-10-03T15:27:39Z Identification of polydisperse RNA in the cytoplasm of neurons isolated from the immature brain cortex Azcurra, J.M. Carrasco, A.E. rna animal experiment brain cortex central nervous system nerve cell nervous system rat Animal Cerebral Cortex Cytoplasm Electrophoresis, Polyacrylamide Gel Molecular Weight Rats RNA RNA, Ribosomal The cytoplasmic RNA of 10-day-old rats was studied in bulk-isolated, cortical neurons, obtained under conditions which minimize nuclear contamination. Two RNA fractions, one enriched in rRNA and the other in "polydisperse" RNA, were obtained by differential extraction with phenol. Gel electrophoresis and pulse labelling with 5-[3H]uridine were used to confirm the delayed appearance of newly synthesized rRNA in the cytoplasm and to demonstrate its stability. "Polydisperse" RNA appeared in the cytoplasm earlier and had a shorter half-life than rRNA. A wide range of molecular weights for this RNA was found with no predominant individual species. A set of cytoplasmic RNA components of molecular size between 28S and 18S was also present probably reflecting the in vivo degradation of rRNA. The significance of the unexpectedly high amounts of neuronal polydisperse RNA is discussed. © 1978 Plenum Publishing Corporation. Fil:Azcurra, J.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_03643190_v3_n5_p521_Azcurra
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic rna
animal experiment
brain cortex
central nervous system
nerve cell
nervous system
rat
Animal
Cerebral Cortex
Cytoplasm
Electrophoresis, Polyacrylamide Gel
Molecular Weight
Rats
RNA
RNA, Ribosomal
spellingShingle rna
animal experiment
brain cortex
central nervous system
nerve cell
nervous system
rat
Animal
Cerebral Cortex
Cytoplasm
Electrophoresis, Polyacrylamide Gel
Molecular Weight
Rats
RNA
RNA, Ribosomal
Azcurra, J.M.
Carrasco, A.E.
Identification of polydisperse RNA in the cytoplasm of neurons isolated from the immature brain cortex
topic_facet rna
animal experiment
brain cortex
central nervous system
nerve cell
nervous system
rat
Animal
Cerebral Cortex
Cytoplasm
Electrophoresis, Polyacrylamide Gel
Molecular Weight
Rats
RNA
RNA, Ribosomal
description The cytoplasmic RNA of 10-day-old rats was studied in bulk-isolated, cortical neurons, obtained under conditions which minimize nuclear contamination. Two RNA fractions, one enriched in rRNA and the other in "polydisperse" RNA, were obtained by differential extraction with phenol. Gel electrophoresis and pulse labelling with 5-[3H]uridine were used to confirm the delayed appearance of newly synthesized rRNA in the cytoplasm and to demonstrate its stability. "Polydisperse" RNA appeared in the cytoplasm earlier and had a shorter half-life than rRNA. A wide range of molecular weights for this RNA was found with no predominant individual species. A set of cytoplasmic RNA components of molecular size between 28S and 18S was also present probably reflecting the in vivo degradation of rRNA. The significance of the unexpectedly high amounts of neuronal polydisperse RNA is discussed. © 1978 Plenum Publishing Corporation.
format JOUR
author Azcurra, J.M.
Carrasco, A.E.
author_facet Azcurra, J.M.
Carrasco, A.E.
author_sort Azcurra, J.M.
title Identification of polydisperse RNA in the cytoplasm of neurons isolated from the immature brain cortex
title_short Identification of polydisperse RNA in the cytoplasm of neurons isolated from the immature brain cortex
title_full Identification of polydisperse RNA in the cytoplasm of neurons isolated from the immature brain cortex
title_fullStr Identification of polydisperse RNA in the cytoplasm of neurons isolated from the immature brain cortex
title_full_unstemmed Identification of polydisperse RNA in the cytoplasm of neurons isolated from the immature brain cortex
title_sort identification of polydisperse rna in the cytoplasm of neurons isolated from the immature brain cortex
url http://hdl.handle.net/20.500.12110/paper_03643190_v3_n5_p521_Azcurra
work_keys_str_mv AT azcurrajm identificationofpolydispersernainthecytoplasmofneuronsisolatedfromtheimmaturebraincortex
AT carrascoae identificationofpolydispersernainthecytoplasmofneuronsisolatedfromtheimmaturebraincortex
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