Decreased phosphorylation of GAP-43/B-50 in striatal synaptic plasma membranes after circling motor activity

The effects of spontaneous circling motor activity on the in vitro phosphorylation of the protein kinase C substrate GAP-43/B-50 was studied on striatal membranes of developing rats (30 days of age). At this time of postnatal development, permanent plastic changes in cholinergic and dopaminergic sys...

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Autores principales:	Paratcha, G.Ch., Ibarra, G.R., Cabrera, R., Azcurra, J.M.
Formato:	JOUR
Materias:	Circling motor activity and development GAP-43/B-50 PKC Rat striatum SPM 1 (5 isoquinolinesulfonyl) 2 methylpiperazine brain receptor cipermethrin egtazic acid heparin neuromodulin okadaic acid phorbol ester phosphate phosphorus 32 protein kinase c staurosporine animal experiment animal tissue article cholinergic system circling behavior controlled study corpus striatum dopaminergic system enzyme activity exercise male motor activity newborn nonhuman postnatal development priority journal protein phosphorylation rat running synaptic membrane Animals Cell Membrane Corpus Striatum GAP-43 Protein Male Motor Activity Phosphorylation Rats Rats, Sprague-Dawley Synaptic Membranes Animalia
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_03643190_v23_n10_p1241_Paratcha
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_03643190_v23_n10_p1241_Paratcha
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spelling	todo:paper_03643190_v23_n10_p1241_Paratcha2023-10-03T15:27:36Z Decreased phosphorylation of GAP-43/B-50 in striatal synaptic plasma membranes after circling motor activity Paratcha, G.Ch. Ibarra, G.R. Cabrera, R. Azcurra, J.M. Circling motor activity and development GAP-43/B-50 PKC Rat striatum SPM 1 (5 isoquinolinesulfonyl) 2 methylpiperazine brain receptor cipermethrin egtazic acid heparin neuromodulin okadaic acid phorbol ester phosphate phosphorus 32 protein kinase c staurosporine animal experiment animal tissue article cholinergic system circling behavior controlled study corpus striatum dopaminergic system enzyme activity exercise male motor activity newborn nonhuman postnatal development priority journal protein phosphorylation rat running synaptic membrane Animals Cell Membrane Corpus Striatum GAP-43 Protein Male Motor Activity Phosphorylation Rats Rats, Sprague-Dawley Synaptic Membranes Animalia The effects of spontaneous circling motor activity on the in vitro phosphorylation of the protein kinase C substrate GAP-43/B-50 was studied on striatal membranes of developing rats (30 days of age). At this time of postnatal development, permanent plastic changes in cholinergic and dopaminergic systems are produced by physiological motor activity. Exercised animals showed a significant reduction of 31% in the level of GAP-43/B-50 endogenous phosphorylation in the contralateral striatum respect to the ipsilateral side (P < 0.01), while control animals did not show asymmetric differences. Compared to controls, the contralateral striatum of exercised animals showed a 33% reduction in the incorporation of 32P-phosphate into GAP-43/B-50 30 minutes post-exercise (P < 0.01). This change in GAP-43/B-50 phosphorylation was correlated with the running speed developed by the animals (r:0.8986, P = 0.015). GAP-43/B-50 immunoblots revealed no changes in the amount of this protein in any group. Moreover, a significant variation of 25% (P < 0.05) in the PKC activity was seen between both exercised striata. Interhemispheric differences were not found in control animals. We conclude that endogenous phosphorylation of this protein is also altered by motor activity in the same period that permanent changes in striatal neuroreceptors are triggered after motor training. Fil:Paratcha, G.Ch. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Azcurra, J.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_03643190_v23_n10_p1241_Paratcha
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Circling motor activity and development GAP-43/B-50 PKC Rat striatum SPM 1 (5 isoquinolinesulfonyl) 2 methylpiperazine brain receptor cipermethrin egtazic acid heparin neuromodulin okadaic acid phorbol ester phosphate phosphorus 32 protein kinase c staurosporine animal experiment animal tissue article cholinergic system circling behavior controlled study corpus striatum dopaminergic system enzyme activity exercise male motor activity newborn nonhuman postnatal development priority journal protein phosphorylation rat running synaptic membrane Animals Cell Membrane Corpus Striatum GAP-43 Protein Male Motor Activity Phosphorylation Rats Rats, Sprague-Dawley Synaptic Membranes Animalia
spellingShingle	Circling motor activity and development GAP-43/B-50 PKC Rat striatum SPM 1 (5 isoquinolinesulfonyl) 2 methylpiperazine brain receptor cipermethrin egtazic acid heparin neuromodulin okadaic acid phorbol ester phosphate phosphorus 32 protein kinase c staurosporine animal experiment animal tissue article cholinergic system circling behavior controlled study corpus striatum dopaminergic system enzyme activity exercise male motor activity newborn nonhuman postnatal development priority journal protein phosphorylation rat running synaptic membrane Animals Cell Membrane Corpus Striatum GAP-43 Protein Male Motor Activity Phosphorylation Rats Rats, Sprague-Dawley Synaptic Membranes Animalia Paratcha, G.Ch. Ibarra, G.R. Cabrera, R. Azcurra, J.M. Decreased phosphorylation of GAP-43/B-50 in striatal synaptic plasma membranes after circling motor activity
topic_facet	Circling motor activity and development GAP-43/B-50 PKC Rat striatum SPM 1 (5 isoquinolinesulfonyl) 2 methylpiperazine brain receptor cipermethrin egtazic acid heparin neuromodulin okadaic acid phorbol ester phosphate phosphorus 32 protein kinase c staurosporine animal experiment animal tissue article cholinergic system circling behavior controlled study corpus striatum dopaminergic system enzyme activity exercise male motor activity newborn nonhuman postnatal development priority journal protein phosphorylation rat running synaptic membrane Animals Cell Membrane Corpus Striatum GAP-43 Protein Male Motor Activity Phosphorylation Rats Rats, Sprague-Dawley Synaptic Membranes Animalia
description	The effects of spontaneous circling motor activity on the in vitro phosphorylation of the protein kinase C substrate GAP-43/B-50 was studied on striatal membranes of developing rats (30 days of age). At this time of postnatal development, permanent plastic changes in cholinergic and dopaminergic systems are produced by physiological motor activity. Exercised animals showed a significant reduction of 31% in the level of GAP-43/B-50 endogenous phosphorylation in the contralateral striatum respect to the ipsilateral side (P < 0.01), while control animals did not show asymmetric differences. Compared to controls, the contralateral striatum of exercised animals showed a 33% reduction in the incorporation of 32P-phosphate into GAP-43/B-50 30 minutes post-exercise (P < 0.01). This change in GAP-43/B-50 phosphorylation was correlated with the running speed developed by the animals (r:0.8986, P = 0.015). GAP-43/B-50 immunoblots revealed no changes in the amount of this protein in any group. Moreover, a significant variation of 25% (P < 0.05) in the PKC activity was seen between both exercised striata. Interhemispheric differences were not found in control animals. We conclude that endogenous phosphorylation of this protein is also altered by motor activity in the same period that permanent changes in striatal neuroreceptors are triggered after motor training.
format	JOUR
author	Paratcha, G.Ch. Ibarra, G.R. Cabrera, R. Azcurra, J.M.
author_facet	Paratcha, G.Ch. Ibarra, G.R. Cabrera, R. Azcurra, J.M.
author_sort	Paratcha, G.Ch.
title	Decreased phosphorylation of GAP-43/B-50 in striatal synaptic plasma membranes after circling motor activity
title_short	Decreased phosphorylation of GAP-43/B-50 in striatal synaptic plasma membranes after circling motor activity
title_full	Decreased phosphorylation of GAP-43/B-50 in striatal synaptic plasma membranes after circling motor activity
title_fullStr	Decreased phosphorylation of GAP-43/B-50 in striatal synaptic plasma membranes after circling motor activity
title_full_unstemmed	Decreased phosphorylation of GAP-43/B-50 in striatal synaptic plasma membranes after circling motor activity
title_sort	decreased phosphorylation of gap-43/b-50 in striatal synaptic plasma membranes after circling motor activity
url	http://hdl.handle.net/20.500.12110/paper_03643190_v23_n10_p1241_Paratcha
work_keys_str_mv	AT paratchagch decreasedphosphorylationofgap43b50instriatalsynapticplasmamembranesaftercirclingmotoractivity AT ibarragr decreasedphosphorylationofgap43b50instriatalsynapticplasmamembranesaftercirclingmotoractivity AT cabrerar decreasedphosphorylationofgap43b50instriatalsynapticplasmamembranesaftercirclingmotoractivity AT azcurrajm decreasedphosphorylationofgap43b50instriatalsynapticplasmamembranesaftercirclingmotoractivity
_version_	1807324595479379968

Decreased phosphorylation of GAP-43/B-50 in striatal synaptic plasma membranes after circling motor activity

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