Regulation of ion transport by pH and [HCO3 -] in isolated gills of the crab Neohelice (Chasmagnathus) granulata
Posterior isolated gills of Neohelice (Chasmagnathus) granulatus were symmetrically perfused with hemolymph-like saline of varying [HCO 3 -] and pH. Elevating [HCO3 -] in the saline from 2.5 to 12.5 mmol/l (pH 7.75 in both cases) induced a significant increase in the transepithelial potential differ...
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todo:paper_03636119_v294_n3_pR1033_Tresguerres2023-10-03T15:27:30Z Regulation of ion transport by pH and [HCO3 -] in isolated gills of the crab Neohelice (Chasmagnathus) granulata Tresguerres, M. Parks, S.K. Sabatini, S.E. Goss, G.G. Luquet, C.M. Acetazolamide Acid/base regulation Amiloride Bafilomycin Carbonic anhydrase Electrogenic Na+/H+ exchanger HCO3 - secretion V-H +-ATPase acetazolamide adenosine triphosphatase (potassium sodium) amiloride bafilomycin bicarbonate carbonate dehydratase ouabain proton transporting adenosine triphosphatase sodium chloride sodium proton exchange protein acid base balance acid secretion acidity alkalinity animal experiment animal tissue article cell membrane potential crab epithelium cell gill immunoreactivity ion transport Neohelice granulata nonhuman perfusion priority journal 4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid 4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid Animals Bicarbonates Biological Transport, Active Brachyura Diuretics Enzyme Inhibitors Ganglia, Invertebrate Gills Hydrogen-Ion Concentration Immunohistochemistry Indicators and Reagents Ions Macrolides Membrane Potentials Proton-Translocating ATPases Solutions Posterior isolated gills of Neohelice (Chasmagnathus) granulatus were symmetrically perfused with hemolymph-like saline of varying [HCO 3 -] and pH. Elevating [HCO3 -] in the saline from 2.5 to 12.5 mmol/l (pH 7.75 in both cases) induced a significant increase in the transepithelial potential difference (Vte), a measure of ion transport. The elevation in [HCO3 -] also induced a switch from acid secretion (-43.7 ± 22.5 μequiv·kg -1·h-1) in controls to base secretion (84.7 ± 14.4 μequiv·kg-1·h-1). The HCO3 --induced Vte increase was inhibited by basolateral acetazolamide (200 μmol/l), amiloride (1 mmol/l), and ouabain (5 mmol/l) but not by bafilomycin (100 nmol/l). The Vte response to HCO3 - did not take place in Cl--free conditions; however, it was unaffected by apical SITS (2 mmol/l) or DIDS (1 mmol/l). A decrease in pH from 7.75 to 7.45 pH units in the perfusate also induced a significant increase in Vte, which was matched by a net increase in acid secretion of 67.8 ± 18.4 μequiv kg-1 h-1. This stimulation was sensitive to basolateral acetazolamide, bafilomycin, DIDS, and Na+-free conditions, but it still took place in Cl--free saline. Therefore, the cellular response to low pH is different from the HCO3 --stimulated response. We also report V-H+-ATPase- and Na+-K+-ATPase-like immunoreactivity in gill sections for the first time in this crab. Our results suggest that carbonic anhydrase (CA), basolateral Na+/H+ exchangers and Na+-K+-ATPase and apical anion exchangers participate in the HCO3 --stimulated response, while CA, apical V-H+-ATPase and basolateral HCO3 --dependent cotransporters mediate the response to low pH. Copyright © 2008 the American Physiological Society. Fil:Tresguerres, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Sabatini, S.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Luquet, C.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_03636119_v294_n3_pR1033_Tresguerres |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Acetazolamide Acid/base regulation Amiloride Bafilomycin Carbonic anhydrase Electrogenic Na+/H+ exchanger HCO3 - secretion V-H +-ATPase acetazolamide adenosine triphosphatase (potassium sodium) amiloride bafilomycin bicarbonate carbonate dehydratase ouabain proton transporting adenosine triphosphatase sodium chloride sodium proton exchange protein acid base balance acid secretion acidity alkalinity animal experiment animal tissue article cell membrane potential crab epithelium cell gill immunoreactivity ion transport Neohelice granulata nonhuman perfusion priority journal 4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid 4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid Animals Bicarbonates Biological Transport, Active Brachyura Diuretics Enzyme Inhibitors Ganglia, Invertebrate Gills Hydrogen-Ion Concentration Immunohistochemistry Indicators and Reagents Ions Macrolides Membrane Potentials Proton-Translocating ATPases Solutions |
| spellingShingle |
Acetazolamide Acid/base regulation Amiloride Bafilomycin Carbonic anhydrase Electrogenic Na+/H+ exchanger HCO3 - secretion V-H +-ATPase acetazolamide adenosine triphosphatase (potassium sodium) amiloride bafilomycin bicarbonate carbonate dehydratase ouabain proton transporting adenosine triphosphatase sodium chloride sodium proton exchange protein acid base balance acid secretion acidity alkalinity animal experiment animal tissue article cell membrane potential crab epithelium cell gill immunoreactivity ion transport Neohelice granulata nonhuman perfusion priority journal 4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid 4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid Animals Bicarbonates Biological Transport, Active Brachyura Diuretics Enzyme Inhibitors Ganglia, Invertebrate Gills Hydrogen-Ion Concentration Immunohistochemistry Indicators and Reagents Ions Macrolides Membrane Potentials Proton-Translocating ATPases Solutions Tresguerres, M. Parks, S.K. Sabatini, S.E. Goss, G.G. Luquet, C.M. Regulation of ion transport by pH and [HCO3 -] in isolated gills of the crab Neohelice (Chasmagnathus) granulata |
| topic_facet |
Acetazolamide Acid/base regulation Amiloride Bafilomycin Carbonic anhydrase Electrogenic Na+/H+ exchanger HCO3 - secretion V-H +-ATPase acetazolamide adenosine triphosphatase (potassium sodium) amiloride bafilomycin bicarbonate carbonate dehydratase ouabain proton transporting adenosine triphosphatase sodium chloride sodium proton exchange protein acid base balance acid secretion acidity alkalinity animal experiment animal tissue article cell membrane potential crab epithelium cell gill immunoreactivity ion transport Neohelice granulata nonhuman perfusion priority journal 4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid 4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid Animals Bicarbonates Biological Transport, Active Brachyura Diuretics Enzyme Inhibitors Ganglia, Invertebrate Gills Hydrogen-Ion Concentration Immunohistochemistry Indicators and Reagents Ions Macrolides Membrane Potentials Proton-Translocating ATPases Solutions |
| description |
Posterior isolated gills of Neohelice (Chasmagnathus) granulatus were symmetrically perfused with hemolymph-like saline of varying [HCO 3 -] and pH. Elevating [HCO3 -] in the saline from 2.5 to 12.5 mmol/l (pH 7.75 in both cases) induced a significant increase in the transepithelial potential difference (Vte), a measure of ion transport. The elevation in [HCO3 -] also induced a switch from acid secretion (-43.7 ± 22.5 μequiv·kg -1·h-1) in controls to base secretion (84.7 ± 14.4 μequiv·kg-1·h-1). The HCO3 --induced Vte increase was inhibited by basolateral acetazolamide (200 μmol/l), amiloride (1 mmol/l), and ouabain (5 mmol/l) but not by bafilomycin (100 nmol/l). The Vte response to HCO3 - did not take place in Cl--free conditions; however, it was unaffected by apical SITS (2 mmol/l) or DIDS (1 mmol/l). A decrease in pH from 7.75 to 7.45 pH units in the perfusate also induced a significant increase in Vte, which was matched by a net increase in acid secretion of 67.8 ± 18.4 μequiv kg-1 h-1. This stimulation was sensitive to basolateral acetazolamide, bafilomycin, DIDS, and Na+-free conditions, but it still took place in Cl--free saline. Therefore, the cellular response to low pH is different from the HCO3 --stimulated response. We also report V-H+-ATPase- and Na+-K+-ATPase-like immunoreactivity in gill sections for the first time in this crab. Our results suggest that carbonic anhydrase (CA), basolateral Na+/H+ exchangers and Na+-K+-ATPase and apical anion exchangers participate in the HCO3 --stimulated response, while CA, apical V-H+-ATPase and basolateral HCO3 --dependent cotransporters mediate the response to low pH. Copyright © 2008 the American Physiological Society. |
| format |
JOUR |
| author |
Tresguerres, M. Parks, S.K. Sabatini, S.E. Goss, G.G. Luquet, C.M. |
| author_facet |
Tresguerres, M. Parks, S.K. Sabatini, S.E. Goss, G.G. Luquet, C.M. |
| author_sort |
Tresguerres, M. |
| title |
Regulation of ion transport by pH and [HCO3 -] in isolated gills of the crab Neohelice (Chasmagnathus) granulata |
| title_short |
Regulation of ion transport by pH and [HCO3 -] in isolated gills of the crab Neohelice (Chasmagnathus) granulata |
| title_full |
Regulation of ion transport by pH and [HCO3 -] in isolated gills of the crab Neohelice (Chasmagnathus) granulata |
| title_fullStr |
Regulation of ion transport by pH and [HCO3 -] in isolated gills of the crab Neohelice (Chasmagnathus) granulata |
| title_full_unstemmed |
Regulation of ion transport by pH and [HCO3 -] in isolated gills of the crab Neohelice (Chasmagnathus) granulata |
| title_sort |
regulation of ion transport by ph and [hco3 -] in isolated gills of the crab neohelice (chasmagnathus) granulata |
| url |
http://hdl.handle.net/20.500.12110/paper_03636119_v294_n3_pR1033_Tresguerres |
| work_keys_str_mv |
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