Combined effect of nisin and pulsed electric fields on the inactivation of Escherichia coli

The Doehlert design was applied in order to investigate the combined effect of nisin and high voltage pulsed electric fields (PEF) on the inactivation of Escherichia coli in simulated milk ultrafiltrate media. Nisin alone was totally inactivated by PEF, but in the presence of bacterial cells a prote...

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Detalles Bibliográficos
Autores principales: Terebiznik, M.R., Jagus, R.J., Cerrutti, P., De Huergo, M.S., Pilosof, A.M.R.
Formato: JOUR
Materias:
antibacterial activity
bacterium culture
culture medium
Escherichia coli
nisin
pulsed electric field
simulation
Bacteria (microorganisms)
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_0362028X_v63_n6_p741_Terebiznik
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_0362028X_v63_n6_p741_Terebiznik
record_format dspace
spelling todo:paper_0362028X_v63_n6_p741_Terebiznik2023-10-03T15:26:48Z Combined effect of nisin and pulsed electric fields on the inactivation of Escherichia coli Terebiznik, M.R. Jagus, R.J. Cerrutti, P. De Huergo, M.S. Pilosof, A.M.R. antibacterial activity bacterium culture culture medium Escherichia coli nisin pulsed electric field simulation Bacteria (microorganisms) Escherichia coli The Doehlert design was applied in order to investigate the combined effect of nisin and high voltage pulsed electric fields (PEF) on the inactivation of Escherichia coli in simulated milk ultrafiltrate media. Nisin alone was totally inactivated by PEF, but in the presence of bacterial cells a protective effect was observed. However, the effectiveness of nisin was still decreased when bacterial cells were subjected to the combined treatment. In spite of this phenomenon, an almost additive response emerged as a consequence of the combined treatment. A 4-log cycle reduction may be accomplished with around 1,000 IU/ml (7.15 μM) of nisin and three pulses of 11.25 kV/cm or 500 IU/ml for five pulses of the same intensity. The observed efficacy arising from the combination of both treatments suggests the possibility of using PEF for improving the action spectrum of natural antimicrobials. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_0362028X_v63_n6_p741_Terebiznik
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic antibacterial activity
bacterium culture
culture medium
Escherichia coli
nisin
pulsed electric field
simulation
Bacteria (microorganisms)
Escherichia coli
spellingShingle antibacterial activity
bacterium culture
culture medium
Escherichia coli
nisin
pulsed electric field
simulation
Bacteria (microorganisms)
Escherichia coli
Terebiznik, M.R.
Jagus, R.J.
Cerrutti, P.
De Huergo, M.S.
Pilosof, A.M.R.
Combined effect of nisin and pulsed electric fields on the inactivation of Escherichia coli
topic_facet antibacterial activity
bacterium culture
culture medium
Escherichia coli
nisin
pulsed electric field
simulation
Bacteria (microorganisms)
Escherichia coli
description The Doehlert design was applied in order to investigate the combined effect of nisin and high voltage pulsed electric fields (PEF) on the inactivation of Escherichia coli in simulated milk ultrafiltrate media. Nisin alone was totally inactivated by PEF, but in the presence of bacterial cells a protective effect was observed. However, the effectiveness of nisin was still decreased when bacterial cells were subjected to the combined treatment. In spite of this phenomenon, an almost additive response emerged as a consequence of the combined treatment. A 4-log cycle reduction may be accomplished with around 1,000 IU/ml (7.15 μM) of nisin and three pulses of 11.25 kV/cm or 500 IU/ml for five pulses of the same intensity. The observed efficacy arising from the combination of both treatments suggests the possibility of using PEF for improving the action spectrum of natural antimicrobials.
format JOUR
author Terebiznik, M.R.
Jagus, R.J.
Cerrutti, P.
De Huergo, M.S.
Pilosof, A.M.R.
author_facet Terebiznik, M.R.
Jagus, R.J.
Cerrutti, P.
De Huergo, M.S.
Pilosof, A.M.R.
author_sort Terebiznik, M.R.
title Combined effect of nisin and pulsed electric fields on the inactivation of Escherichia coli
title_short Combined effect of nisin and pulsed electric fields on the inactivation of Escherichia coli
title_full Combined effect of nisin and pulsed electric fields on the inactivation of Escherichia coli
title_fullStr Combined effect of nisin and pulsed electric fields on the inactivation of Escherichia coli
title_full_unstemmed Combined effect of nisin and pulsed electric fields on the inactivation of Escherichia coli
title_sort combined effect of nisin and pulsed electric fields on the inactivation of escherichia coli
url http://hdl.handle.net/20.500.12110/paper_0362028X_v63_n6_p741_Terebiznik
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AT cerruttip combinedeffectofnisinandpulsedelectricfieldsontheinactivationofescherichiacoli
AT dehuergoms combinedeffectofnisinandpulsedelectricfieldsontheinactivationofescherichiacoli
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