Subcellular fractionation and association with the cytoskeleton of messengers encoding myelin proteins

The targeting of polypeptides to restricted cytoplasmic domains by means of mRNA sorting is a widespread phenomena utilized by many cell types. In the central nervous system, in situ hybridization analysis has shown previously that the mRNAs encoding several myelin-specific proteins are specifically...

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Autores principales:	Boccaccio, G.L., Carminatti, H., Colman, D.R.
Formato:	JOUR
Materias:	MBP mRNA MOBP mRNA MRNA transport myelin basic protein RNA animal cell article cell fractionation cytoskeleton in situ hybridization mouse myelin sheath nonhuman priority journal protein determination protein targeting RNA analysis RNA splicing RNA translation RNA transport Alternative Splicing Animals Blotting, Northern Blotting, Western Cell Membrane Cytoskeleton Exons Immunoblotting Mice Mice, Inbred C57BL Myelin Proteins Myelin Sheath Oligodendroglia Osmotic Pressure Plasmids Reverse Transcriptase Polymerase Chain Reaction RNA Probes RNA, Messenger Subcellular Fractions
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_03604012_v58_n4_p480_Boccaccio
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_03604012_v58_n4_p480_Boccaccio
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spelling	todo:paper_03604012_v58_n4_p480_Boccaccio2023-10-03T15:26:33Z Subcellular fractionation and association with the cytoskeleton of messengers encoding myelin proteins Boccaccio, G.L. Carminatti, H. Colman, D.R. MBP mRNA MOBP mRNA MRNA transport myelin basic protein RNA animal cell article cell fractionation cytoskeleton in situ hybridization mouse myelin sheath nonhuman priority journal protein determination protein targeting RNA analysis RNA splicing RNA translation RNA transport Alternative Splicing Animals Blotting, Northern Blotting, Western Cell Membrane Cytoskeleton Exons Immunoblotting Mice Mice, Inbred C57BL Myelin Proteins Myelin Sheath Oligodendroglia Osmotic Pressure Plasmids Reverse Transcriptase Polymerase Chain Reaction RNA Probes RNA, Messenger Subcellular Fractions The targeting of polypeptides to restricted cytoplasmic domains by means of mRNA sorting is a widespread phenomena utilized by many cell types. In the central nervous system, in situ hybridization analysis has shown previously that the mRNAs encoding several myelin-specific proteins are specifically located within the myelinating processes of oligodendrocytes. Here, by means of biochemical and subcellular fractionation methods, we show that a myelin fraction is selectively enriched in those mRNAs. The four major myelin basic protein (MBP) mRNAs that arise by alternative splicing of exons II and VI of the MBP gene are concentrated in this subcellular fraction. Furthermore, an interaction of MBP and MOBP 81A mRNAs with the cytoskeleton was observed. This interaction might serve to mediate the anchoring of these messengers after translocation to the subcellular site of translation. Fil:Boccaccio, G.L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Carminatti, H. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_03604012_v58_n4_p480_Boccaccio
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	MBP mRNA MOBP mRNA MRNA transport myelin basic protein RNA animal cell article cell fractionation cytoskeleton in situ hybridization mouse myelin sheath nonhuman priority journal protein determination protein targeting RNA analysis RNA splicing RNA translation RNA transport Alternative Splicing Animals Blotting, Northern Blotting, Western Cell Membrane Cytoskeleton Exons Immunoblotting Mice Mice, Inbred C57BL Myelin Proteins Myelin Sheath Oligodendroglia Osmotic Pressure Plasmids Reverse Transcriptase Polymerase Chain Reaction RNA Probes RNA, Messenger Subcellular Fractions
spellingShingle	MBP mRNA MOBP mRNA MRNA transport myelin basic protein RNA animal cell article cell fractionation cytoskeleton in situ hybridization mouse myelin sheath nonhuman priority journal protein determination protein targeting RNA analysis RNA splicing RNA translation RNA transport Alternative Splicing Animals Blotting, Northern Blotting, Western Cell Membrane Cytoskeleton Exons Immunoblotting Mice Mice, Inbred C57BL Myelin Proteins Myelin Sheath Oligodendroglia Osmotic Pressure Plasmids Reverse Transcriptase Polymerase Chain Reaction RNA Probes RNA, Messenger Subcellular Fractions Boccaccio, G.L. Carminatti, H. Colman, D.R. Subcellular fractionation and association with the cytoskeleton of messengers encoding myelin proteins
topic_facet	MBP mRNA MOBP mRNA MRNA transport myelin basic protein RNA animal cell article cell fractionation cytoskeleton in situ hybridization mouse myelin sheath nonhuman priority journal protein determination protein targeting RNA analysis RNA splicing RNA translation RNA transport Alternative Splicing Animals Blotting, Northern Blotting, Western Cell Membrane Cytoskeleton Exons Immunoblotting Mice Mice, Inbred C57BL Myelin Proteins Myelin Sheath Oligodendroglia Osmotic Pressure Plasmids Reverse Transcriptase Polymerase Chain Reaction RNA Probes RNA, Messenger Subcellular Fractions
description	The targeting of polypeptides to restricted cytoplasmic domains by means of mRNA sorting is a widespread phenomena utilized by many cell types. In the central nervous system, in situ hybridization analysis has shown previously that the mRNAs encoding several myelin-specific proteins are specifically located within the myelinating processes of oligodendrocytes. Here, by means of biochemical and subcellular fractionation methods, we show that a myelin fraction is selectively enriched in those mRNAs. The four major myelin basic protein (MBP) mRNAs that arise by alternative splicing of exons II and VI of the MBP gene are concentrated in this subcellular fraction. Furthermore, an interaction of MBP and MOBP 81A mRNAs with the cytoskeleton was observed. This interaction might serve to mediate the anchoring of these messengers after translocation to the subcellular site of translation.
format	JOUR
author	Boccaccio, G.L. Carminatti, H. Colman, D.R.
author_facet	Boccaccio, G.L. Carminatti, H. Colman, D.R.
author_sort	Boccaccio, G.L.
title	Subcellular fractionation and association with the cytoskeleton of messengers encoding myelin proteins
title_short	Subcellular fractionation and association with the cytoskeleton of messengers encoding myelin proteins
title_full	Subcellular fractionation and association with the cytoskeleton of messengers encoding myelin proteins
title_fullStr	Subcellular fractionation and association with the cytoskeleton of messengers encoding myelin proteins
title_full_unstemmed	Subcellular fractionation and association with the cytoskeleton of messengers encoding myelin proteins
title_sort	subcellular fractionation and association with the cytoskeleton of messengers encoding myelin proteins
url	http://hdl.handle.net/20.500.12110/paper_03604012_v58_n4_p480_Boccaccio
work_keys_str_mv	AT boccacciogl subcellularfractionationandassociationwiththecytoskeletonofmessengersencodingmyelinproteins AT carminattih subcellularfractionationandassociationwiththecytoskeletonofmessengersencodingmyelinproteins AT colmandr subcellularfractionationandassociationwiththecytoskeletonofmessengersencodingmyelinproteins
_version_	1807322824965095424

Subcellular fractionation and association with the cytoskeleton of messengers encoding myelin proteins

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