Induction and Rejoining of DNA Double Strand Breaks Assessed by H2AX Phosphorylation in Melanoma Cells Irradiated with Proton and Lithium Beams

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Purpose: The aim of this study was to evaluate the induction and rejoining of DNA double strand breaks (DSBs) in melanoma cells exposed to low and high linear energy transfer (LET) radiation. Methods and Materials: DSBs and survival were determined as a function of dose in melanoma cells (B16-F0) ir...

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Autores principales:	Ibañez, I.L., Bracalente, C., Molinari, B.L., Palmieri, M.A., Policastro, L., Kreiner, A.J., Burlón, A.A., Valda, A., Navalesi, D., Davidson, J., Davidson, M., Vázquez, M., Ozafrán, M., Durán, H.
Formato:	JOUR
Materias:	γH2AX DNA DSB Ionizing radiation LET Melanoma cells Clonogenic assays DNA double strand breaks Gamma source High linear energy transfers In-cell LET radiation Linear-quadratic models Low-LET radiation Monoenergetic protons Phosphorylated histone Postirradiation Survival curves Surviving fractions Cell membranes DNA Energy transfer Genes Lithium Nucleic acids Phosphorylation Proton irradiation Protons Radiation shielding Radioactivity Lighting fixtures double stranded DNA histone H2AX animal cell article cancer cell culture cancer radiotherapy cancer survival clonogenic assay DNA determination DNA strand breakage linear energy transfer low energy radiation melanoma melanoma cell mouse nonhuman priority journal protein determination protein phosphorylation proton radiation radiation dose Biological Markers Cell Survival DNA Breaks, Double-Stranded DNA Repair Dose-Response Relationship, Radiation Histones Humans Linear Energy Transfer Melanoma, Experimental Radiation Tolerance Radioisotopes
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_03603016_v74_n4_p1226_Ibanez
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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spelling	todo:paper_03603016_v74_n4_p1226_Ibanez2023-10-03T15:26:20Z Induction and Rejoining of DNA Double Strand Breaks Assessed by H2AX Phosphorylation in Melanoma Cells Irradiated with Proton and Lithium Beams Ibañez, I.L. Bracalente, C. Molinari, B.L. Palmieri, M.A. Policastro, L. Kreiner, A.J. Burlón, A.A. Valda, A. Navalesi, D. Davidson, J. Davidson, M. Vázquez, M. Ozafrán, M. Durán, H. γH2AX DNA DSB Ionizing radiation LET Melanoma cells Clonogenic assays DNA double strand breaks DNA DSB Gamma source High linear energy transfers In-cell LET LET radiation Linear-quadratic models Low-LET radiation Melanoma cells Monoenergetic protons Phosphorylated histone Postirradiation Survival curves Surviving fractions Cell membranes DNA Energy transfer Genes Ionizing radiation Lithium Nucleic acids Phosphorylation Proton irradiation Protons Radiation shielding Radioactivity Lighting fixtures double stranded DNA histone H2AX animal cell article cancer cell culture cancer radiotherapy cancer survival clonogenic assay DNA determination DNA strand breakage linear energy transfer low energy radiation melanoma melanoma cell mouse nonhuman priority journal protein determination protein phosphorylation proton radiation radiation dose Biological Markers Cell Survival DNA Breaks, Double-Stranded DNA Repair Dose-Response Relationship, Radiation Histones Humans Linear Energy Transfer Lithium Melanoma, Experimental Phosphorylation Protons Radiation Tolerance Radioisotopes Purpose: The aim of this study was to evaluate the induction and rejoining of DNA double strand breaks (DSBs) in melanoma cells exposed to low and high linear energy transfer (LET) radiation. Methods and Materials: DSBs and survival were determined as a function of dose in melanoma cells (B16-F0) irradiated with monoenergetic proton and lithium beams and with a gamma source. Survival curves were obtained by clonogenic assay and fitted to the linear-quadratic model. DSBs were evaluated by the detection of phosphorylated histone H2AX (γH2AX) foci at 30 min and 6 h post-irradiation. Results: Survival curves showed the increasing effectiveness of radiation as a function of LET. γH2AX labeling showed an increase in the number of foci vs. dose for all the radiations evaluated. A decrease in the number of foci was found at 6 h post-irradiation for low LET radiation, revealing the repair capacity of DSBs. An increase in the size of γH2AX foci in cells irradiated with lithium beams was found, as compared with gamma and proton irradiations, which could be attributed to the clusters of DSBs induced by high LET radiation. Foci size increased at 6 h post-irradiation for lithium and proton irradiations in relation with persistent DSBs, showing a correlation with surviving fraction. Conclusions: Our results showed the response of B16-F0 cells to charged particle beams evaluated by the detection of γH2AX foci. We conclude that γH2AX foci size is an accurate parameter to correlate the rejoining of DSBs induced by different LET radiations and radiosensitivity. © 2009 Elsevier Inc. All rights reserved. Fil:Molinari, B.L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Palmieri, M.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Policastro, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Kreiner, A.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Burlón, A.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Davidson, J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Davidson, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Durán, H. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_03603016_v74_n4_p1226_Ibanez
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	γH2AX DNA DSB Ionizing radiation LET Melanoma cells Clonogenic assays DNA double strand breaks DNA DSB Gamma source High linear energy transfers In-cell LET LET radiation Linear-quadratic models Low-LET radiation Melanoma cells Monoenergetic protons Phosphorylated histone Postirradiation Survival curves Surviving fractions Cell membranes DNA Energy transfer Genes Ionizing radiation Lithium Nucleic acids Phosphorylation Proton irradiation Protons Radiation shielding Radioactivity Lighting fixtures double stranded DNA histone H2AX animal cell article cancer cell culture cancer radiotherapy cancer survival clonogenic assay DNA determination DNA strand breakage linear energy transfer low energy radiation melanoma melanoma cell mouse nonhuman priority journal protein determination protein phosphorylation proton radiation radiation dose Biological Markers Cell Survival DNA Breaks, Double-Stranded DNA Repair Dose-Response Relationship, Radiation Histones Humans Linear Energy Transfer Lithium Melanoma, Experimental Phosphorylation Protons Radiation Tolerance Radioisotopes
spellingShingle	γH2AX DNA DSB Ionizing radiation LET Melanoma cells Clonogenic assays DNA double strand breaks DNA DSB Gamma source High linear energy transfers In-cell LET LET radiation Linear-quadratic models Low-LET radiation Melanoma cells Monoenergetic protons Phosphorylated histone Postirradiation Survival curves Surviving fractions Cell membranes DNA Energy transfer Genes Ionizing radiation Lithium Nucleic acids Phosphorylation Proton irradiation Protons Radiation shielding Radioactivity Lighting fixtures double stranded DNA histone H2AX animal cell article cancer cell culture cancer radiotherapy cancer survival clonogenic assay DNA determination DNA strand breakage linear energy transfer low energy radiation melanoma melanoma cell mouse nonhuman priority journal protein determination protein phosphorylation proton radiation radiation dose Biological Markers Cell Survival DNA Breaks, Double-Stranded DNA Repair Dose-Response Relationship, Radiation Histones Humans Linear Energy Transfer Lithium Melanoma, Experimental Phosphorylation Protons Radiation Tolerance Radioisotopes Ibañez, I.L. Bracalente, C. Molinari, B.L. Palmieri, M.A. Policastro, L. Kreiner, A.J. Burlón, A.A. Valda, A. Navalesi, D. Davidson, J. Davidson, M. Vázquez, M. Ozafrán, M. Durán, H. Induction and Rejoining of DNA Double Strand Breaks Assessed by H2AX Phosphorylation in Melanoma Cells Irradiated with Proton and Lithium Beams
topic_facet	γH2AX DNA DSB Ionizing radiation LET Melanoma cells Clonogenic assays DNA double strand breaks DNA DSB Gamma source High linear energy transfers In-cell LET LET radiation Linear-quadratic models Low-LET radiation Melanoma cells Monoenergetic protons Phosphorylated histone Postirradiation Survival curves Surviving fractions Cell membranes DNA Energy transfer Genes Ionizing radiation Lithium Nucleic acids Phosphorylation Proton irradiation Protons Radiation shielding Radioactivity Lighting fixtures double stranded DNA histone H2AX animal cell article cancer cell culture cancer radiotherapy cancer survival clonogenic assay DNA determination DNA strand breakage linear energy transfer low energy radiation melanoma melanoma cell mouse nonhuman priority journal protein determination protein phosphorylation proton radiation radiation dose Biological Markers Cell Survival DNA Breaks, Double-Stranded DNA Repair Dose-Response Relationship, Radiation Histones Humans Linear Energy Transfer Lithium Melanoma, Experimental Phosphorylation Protons Radiation Tolerance Radioisotopes
description	Purpose: The aim of this study was to evaluate the induction and rejoining of DNA double strand breaks (DSBs) in melanoma cells exposed to low and high linear energy transfer (LET) radiation. Methods and Materials: DSBs and survival were determined as a function of dose in melanoma cells (B16-F0) irradiated with monoenergetic proton and lithium beams and with a gamma source. Survival curves were obtained by clonogenic assay and fitted to the linear-quadratic model. DSBs were evaluated by the detection of phosphorylated histone H2AX (γH2AX) foci at 30 min and 6 h post-irradiation. Results: Survival curves showed the increasing effectiveness of radiation as a function of LET. γH2AX labeling showed an increase in the number of foci vs. dose for all the radiations evaluated. A decrease in the number of foci was found at 6 h post-irradiation for low LET radiation, revealing the repair capacity of DSBs. An increase in the size of γH2AX foci in cells irradiated with lithium beams was found, as compared with gamma and proton irradiations, which could be attributed to the clusters of DSBs induced by high LET radiation. Foci size increased at 6 h post-irradiation for lithium and proton irradiations in relation with persistent DSBs, showing a correlation with surviving fraction. Conclusions: Our results showed the response of B16-F0 cells to charged particle beams evaluated by the detection of γH2AX foci. We conclude that γH2AX foci size is an accurate parameter to correlate the rejoining of DSBs induced by different LET radiations and radiosensitivity. © 2009 Elsevier Inc. All rights reserved.
format	JOUR
author	Ibañez, I.L. Bracalente, C. Molinari, B.L. Palmieri, M.A. Policastro, L. Kreiner, A.J. Burlón, A.A. Valda, A. Navalesi, D. Davidson, J. Davidson, M. Vázquez, M. Ozafrán, M. Durán, H.
author_facet	Ibañez, I.L. Bracalente, C. Molinari, B.L. Palmieri, M.A. Policastro, L. Kreiner, A.J. Burlón, A.A. Valda, A. Navalesi, D. Davidson, J. Davidson, M. Vázquez, M. Ozafrán, M. Durán, H.
author_sort	Ibañez, I.L.
title	Induction and Rejoining of DNA Double Strand Breaks Assessed by H2AX Phosphorylation in Melanoma Cells Irradiated with Proton and Lithium Beams
title_short	Induction and Rejoining of DNA Double Strand Breaks Assessed by H2AX Phosphorylation in Melanoma Cells Irradiated with Proton and Lithium Beams
title_full	Induction and Rejoining of DNA Double Strand Breaks Assessed by H2AX Phosphorylation in Melanoma Cells Irradiated with Proton and Lithium Beams
title_fullStr	Induction and Rejoining of DNA Double Strand Breaks Assessed by H2AX Phosphorylation in Melanoma Cells Irradiated with Proton and Lithium Beams
title_full_unstemmed	Induction and Rejoining of DNA Double Strand Breaks Assessed by H2AX Phosphorylation in Melanoma Cells Irradiated with Proton and Lithium Beams
title_sort	induction and rejoining of dna double strand breaks assessed by h2ax phosphorylation in melanoma cells irradiated with proton and lithium beams
url	http://hdl.handle.net/20.500.12110/paper_03603016_v74_n4_p1226_Ibanez
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_version_	1807320975403909120

Induction and Rejoining of DNA Double Strand Breaks Assessed by H2AX Phosphorylation in Melanoma Cells Irradiated with Proton and Lithium Beams

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