In Bacillus subtilis DegU-P is a positive regulator of the osmotic response

The osmosensitivity presented by spoOA and degU null mutant strains of Bacillus subtilis pointed to their protein products as essential regulators for the osmotic response. This was further investigated by analyzing their transcription activity. The results showed that both spoOA-lacZ and degSU- lac...

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Autores principales:	Ruzal, S.M., Sanchez-Rivas, C.
Formato:	JOUR
Materias:	amylase bacterial DNA bacterial protein beta galactosidase hypertonic solution proteinase article Bacillus subtilis bacterial growth bacterium mutant bacterium transformation catabolite repression enzyme activity enzyme induction gene activity nonhuman osmoregulation priority journal promoter region protein phosphorylation reporter gene sporogenesis transcription initiation Bacteria (microorganisms) Octodon degus Posibacteria Prokaryota
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_03438651_v37_n6_p368_Ruzal
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_03438651_v37_n6_p368_Ruzal
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spelling	todo:paper_03438651_v37_n6_p368_Ruzal2023-10-03T15:26:15Z In Bacillus subtilis DegU-P is a positive regulator of the osmotic response Ruzal, S.M. Sanchez-Rivas, C. amylase bacterial DNA bacterial protein beta galactosidase hypertonic solution proteinase article Bacillus subtilis bacterial growth bacterium mutant bacterium transformation catabolite repression enzyme activity enzyme induction gene activity nonhuman osmoregulation priority journal promoter region protein phosphorylation reporter gene sporogenesis transcription initiation Bacillus subtilis Bacillus subtilis Bacteria (microorganisms) Octodon degus Posibacteria Prokaryota The osmosensitivity presented by spoOA and degU null mutant strains of Bacillus subtilis pointed to their protein products as essential regulators for the osmotic response. This was further investigated by analyzing their transcription activity. The results showed that both spoOA-lacZ and degSU- lacZ were induced by the hypertonic medium. The actual phosphorylation state of these proteins was also analyzed by the bias of two reporter gene promoters activity (abrB-lacZ and degQ-lacZ). The absence of repression of abrB in hypertonic conditions suggested that SpoOA was not phosphorylated while the derepression of degQ promoter suggested that DegU-P was formed. These results were in accordance with the observed absence of sporulation in hyperosmotic media and semi-constitutive osmotolerance of degU(h) mutant strains known to retain phosphorylated DegU. The failure to secrete proteases and to sporulate in hypertonic media suggested that SpoOA acts through abrB regulation in the prevention of alternate responses. The role of DegU-P as positive regulator of the osmotic response seems to be settled and is discussed. Fil:Ruzal, S.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_03438651_v37_n6_p368_Ruzal
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	amylase bacterial DNA bacterial protein beta galactosidase hypertonic solution proteinase article Bacillus subtilis bacterial growth bacterium mutant bacterium transformation catabolite repression enzyme activity enzyme induction gene activity nonhuman osmoregulation priority journal promoter region protein phosphorylation reporter gene sporogenesis transcription initiation Bacillus subtilis Bacillus subtilis Bacteria (microorganisms) Octodon degus Posibacteria Prokaryota
spellingShingle	amylase bacterial DNA bacterial protein beta galactosidase hypertonic solution proteinase article Bacillus subtilis bacterial growth bacterium mutant bacterium transformation catabolite repression enzyme activity enzyme induction gene activity nonhuman osmoregulation priority journal promoter region protein phosphorylation reporter gene sporogenesis transcription initiation Bacillus subtilis Bacillus subtilis Bacteria (microorganisms) Octodon degus Posibacteria Prokaryota Ruzal, S.M. Sanchez-Rivas, C. In Bacillus subtilis DegU-P is a positive regulator of the osmotic response
topic_facet	amylase bacterial DNA bacterial protein beta galactosidase hypertonic solution proteinase article Bacillus subtilis bacterial growth bacterium mutant bacterium transformation catabolite repression enzyme activity enzyme induction gene activity nonhuman osmoregulation priority journal promoter region protein phosphorylation reporter gene sporogenesis transcription initiation Bacillus subtilis Bacillus subtilis Bacteria (microorganisms) Octodon degus Posibacteria Prokaryota
description	The osmosensitivity presented by spoOA and degU null mutant strains of Bacillus subtilis pointed to their protein products as essential regulators for the osmotic response. This was further investigated by analyzing their transcription activity. The results showed that both spoOA-lacZ and degSU- lacZ were induced by the hypertonic medium. The actual phosphorylation state of these proteins was also analyzed by the bias of two reporter gene promoters activity (abrB-lacZ and degQ-lacZ). The absence of repression of abrB in hypertonic conditions suggested that SpoOA was not phosphorylated while the derepression of degQ promoter suggested that DegU-P was formed. These results were in accordance with the observed absence of sporulation in hyperosmotic media and semi-constitutive osmotolerance of degU(h) mutant strains known to retain phosphorylated DegU. The failure to secrete proteases and to sporulate in hypertonic media suggested that SpoOA acts through abrB regulation in the prevention of alternate responses. The role of DegU-P as positive regulator of the osmotic response seems to be settled and is discussed.
format	JOUR
author	Ruzal, S.M. Sanchez-Rivas, C.
author_facet	Ruzal, S.M. Sanchez-Rivas, C.
author_sort	Ruzal, S.M.
title	In Bacillus subtilis DegU-P is a positive regulator of the osmotic response
title_short	In Bacillus subtilis DegU-P is a positive regulator of the osmotic response
title_full	In Bacillus subtilis DegU-P is a positive regulator of the osmotic response
title_fullStr	In Bacillus subtilis DegU-P is a positive regulator of the osmotic response
title_full_unstemmed	In Bacillus subtilis DegU-P is a positive regulator of the osmotic response
title_sort	in bacillus subtilis degu-p is a positive regulator of the osmotic response
url	http://hdl.handle.net/20.500.12110/paper_03438651_v37_n6_p368_Ruzal
work_keys_str_mv	AT ruzalsm inbacillussubtilisdegupisapositiveregulatoroftheosmoticresponse AT sanchezrivasc inbacillussubtilisdegupisapositiveregulatoroftheosmoticresponse
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In Bacillus subtilis DegU-P is a positive regulator of the osmotic response

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