Effect of surfactants on cellulase production by Nectria catalinensis

The effect of different nonionic surfactants (Tween 80, Tween 20, Triton X-100) and polyethylene glycol (PEG 6000) was tested on cellulolytic enzyme system production. Tween 80 gave the highest yield of endoglucanase, exoglucanase, and cellobiase at the 20th day of growth, presumably by causing incr...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autor principal: Pardo, A.G.
Formato: JOUR
Materias:
beta glucosidase
cellulase
glucan synthase
surfactant
article
cell membrane permeability
enzyme activity
enzyme release
enzyme stability
enzyme synthesis
fungus growth
nonhuman
priority journal
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_03438651_v33_n4_p275_Pardo
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_03438651_v33_n4_p275_Pardo
record_format dspace
spelling todo:paper_03438651_v33_n4_p275_Pardo2023-10-03T15:26:13Z Effect of surfactants on cellulase production by Nectria catalinensis Pardo, A.G. beta glucosidase cellulase glucan synthase surfactant article cell membrane permeability enzyme activity enzyme release enzyme stability enzyme synthesis fungus growth nonhuman priority journal The effect of different nonionic surfactants (Tween 80, Tween 20, Triton X-100) and polyethylene glycol (PEG 6000) was tested on cellulolytic enzyme system production. Tween 80 gave the highest yield of endoglucanase, exoglucanase, and cellobiase at the 20th day of growth, presumably by causing increased permeability of cell membranes and/or by promoting the release of cell-bound enzymes. Maximal yield of endoglucanase was achieved with 1.7 mM Tween 80, whereas exoglucanase and cellobiase were at 0.85 mM. In the same way, this compound increased fungal growth. On the other hand, Tween 20 and Triton X-100 inhibited growth and cellulolytic enzyme production. High yields of endoglucanase and exoglucanase were achieved with PEG 6000 in comparison with the control, presumably by increasing enzyme stability. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_03438651_v33_n4_p275_Pardo
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic beta glucosidase
cellulase
glucan synthase
surfactant
article
cell membrane permeability
enzyme activity
enzyme release
enzyme stability
enzyme synthesis
fungus growth
nonhuman
priority journal
spellingShingle beta glucosidase
cellulase
glucan synthase
surfactant
article
cell membrane permeability
enzyme activity
enzyme release
enzyme stability
enzyme synthesis
fungus growth
nonhuman
priority journal
Pardo, A.G.
Effect of surfactants on cellulase production by Nectria catalinensis
topic_facet beta glucosidase
cellulase
glucan synthase
surfactant
article
cell membrane permeability
enzyme activity
enzyme release
enzyme stability
enzyme synthesis
fungus growth
nonhuman
priority journal
description The effect of different nonionic surfactants (Tween 80, Tween 20, Triton X-100) and polyethylene glycol (PEG 6000) was tested on cellulolytic enzyme system production. Tween 80 gave the highest yield of endoglucanase, exoglucanase, and cellobiase at the 20th day of growth, presumably by causing increased permeability of cell membranes and/or by promoting the release of cell-bound enzymes. Maximal yield of endoglucanase was achieved with 1.7 mM Tween 80, whereas exoglucanase and cellobiase were at 0.85 mM. In the same way, this compound increased fungal growth. On the other hand, Tween 20 and Triton X-100 inhibited growth and cellulolytic enzyme production. High yields of endoglucanase and exoglucanase were achieved with PEG 6000 in comparison with the control, presumably by increasing enzyme stability.
format JOUR
author Pardo, A.G.
author_facet Pardo, A.G.
author_sort Pardo, A.G.
title Effect of surfactants on cellulase production by Nectria catalinensis
title_short Effect of surfactants on cellulase production by Nectria catalinensis
title_full Effect of surfactants on cellulase production by Nectria catalinensis
title_fullStr Effect of surfactants on cellulase production by Nectria catalinensis
title_full_unstemmed Effect of surfactants on cellulase production by Nectria catalinensis
title_sort effect of surfactants on cellulase production by nectria catalinensis
url http://hdl.handle.net/20.500.12110/paper_03438651_v33_n4_p275_Pardo
work_keys_str_mv AT pardoag effectofsurfactantsoncellulaseproductionbynectriacatalinensis
_version_ 1807314537958866944

Ejemplares similares