Detection of single copy sequences using BAC-FISH and C-PRINS techniques in sunflower chromosomes
Bacterial artificial chromosome - fluorescence in situ hybridization (BAC-FISH) and cyclingprimed in situ labeling (C-PRINS) techniques were evaluated for integration of physical and genetic maps of sunflower (Helianthus annuus L.). Single-site SSR markers were selected from three linkage groups of...
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todo:paper_03279545_v35_n1_p19_Talia2023-10-03T15:25:18Z Detection of single copy sequences using BAC-FISH and C-PRINS techniques in sunflower chromosomes Talia, P. Greizerstein, E.J. Hopp, H.E. Paniego, N. Poggio, L. Heinz, R.A. BAC-FISH C-PRINS Physical mapping Single-copy sequences Sunflower plant DNA article bacterial artificial chromosome fluorescence in situ hybridization chromosome identification controlled study cycling primed in situ labeling fluorescence in situ hybridization gene mapping genetic linkage genetic marker nonhuman nucleotide repeat nucleotide sequence plant chromosome primed in situ labeling process optimization quantitative trait locus Sclerotinia sclerotiorum sequence analysis signal detection simple sequence repeat sunflower bacterial artificial chromosome DNA sequence genetics methodology Bacteria (microorganisms) Helianthus Helianthus annuus Base Sequence Chromosomes, Artificial, Bacterial Chromosomes, Plant Genetic Markers Helianthus In Situ Hybridization, Fluorescence Quantitative Trait Loci Sequence Analysis, DNA Bacterial artificial chromosome - fluorescence in situ hybridization (BAC-FISH) and cyclingprimed in situ labeling (C-PRINS) techniques were evaluated for integration of physical and genetic maps of sunflower (Helianthus annuus L.). Single-site SSR markers were selected from three linkage groups of a high-density sunflower genetic map. This selection was based on previously identified QTL associated to S. sclerotiorum. These markers were used to select BACs contaning single copy sequences for BAC-FISH aplication. Blocking of highly dispersed repetitive sunflower sequences reduced unspecific hybridization, and allowed the detection of specific signals for BACs containing SSR markers HA4222 and HA2600, anchored to LG 16 and LG 10, respectively. Single-site FISH signal detection was optimized by adjusting the relative quantity and quality of unlabelled repetitive sequences present in the blocking DNA. The SSR marker ORS1247 anchored to the LG 17 was detected by C-PRINS, which yielded fluorescence signals that were specific and intense. This progress in localizing single-copy sequences using BAC-FISH and indirect CPRINS strategies in sunflower will facilitate the integration of genetic and physical maps, allowing the identification of chromosomes containing key genes and/or QTL associated to agronomic important traits in sunflower. Fil:Talia, P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Greizerstein, E.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Hopp, H.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Poggio, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Heinz, R.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_03279545_v35_n1_p19_Talia |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
BAC-FISH C-PRINS Physical mapping Single-copy sequences Sunflower plant DNA article bacterial artificial chromosome fluorescence in situ hybridization chromosome identification controlled study cycling primed in situ labeling fluorescence in situ hybridization gene mapping genetic linkage genetic marker nonhuman nucleotide repeat nucleotide sequence plant chromosome primed in situ labeling process optimization quantitative trait locus Sclerotinia sclerotiorum sequence analysis signal detection simple sequence repeat sunflower bacterial artificial chromosome DNA sequence genetics methodology Bacteria (microorganisms) Helianthus Helianthus annuus Base Sequence Chromosomes, Artificial, Bacterial Chromosomes, Plant Genetic Markers Helianthus In Situ Hybridization, Fluorescence Quantitative Trait Loci Sequence Analysis, DNA |
| spellingShingle |
BAC-FISH C-PRINS Physical mapping Single-copy sequences Sunflower plant DNA article bacterial artificial chromosome fluorescence in situ hybridization chromosome identification controlled study cycling primed in situ labeling fluorescence in situ hybridization gene mapping genetic linkage genetic marker nonhuman nucleotide repeat nucleotide sequence plant chromosome primed in situ labeling process optimization quantitative trait locus Sclerotinia sclerotiorum sequence analysis signal detection simple sequence repeat sunflower bacterial artificial chromosome DNA sequence genetics methodology Bacteria (microorganisms) Helianthus Helianthus annuus Base Sequence Chromosomes, Artificial, Bacterial Chromosomes, Plant Genetic Markers Helianthus In Situ Hybridization, Fluorescence Quantitative Trait Loci Sequence Analysis, DNA Talia, P. Greizerstein, E.J. Hopp, H.E. Paniego, N. Poggio, L. Heinz, R.A. Detection of single copy sequences using BAC-FISH and C-PRINS techniques in sunflower chromosomes |
| topic_facet |
BAC-FISH C-PRINS Physical mapping Single-copy sequences Sunflower plant DNA article bacterial artificial chromosome fluorescence in situ hybridization chromosome identification controlled study cycling primed in situ labeling fluorescence in situ hybridization gene mapping genetic linkage genetic marker nonhuman nucleotide repeat nucleotide sequence plant chromosome primed in situ labeling process optimization quantitative trait locus Sclerotinia sclerotiorum sequence analysis signal detection simple sequence repeat sunflower bacterial artificial chromosome DNA sequence genetics methodology Bacteria (microorganisms) Helianthus Helianthus annuus Base Sequence Chromosomes, Artificial, Bacterial Chromosomes, Plant Genetic Markers Helianthus In Situ Hybridization, Fluorescence Quantitative Trait Loci Sequence Analysis, DNA |
| description |
Bacterial artificial chromosome - fluorescence in situ hybridization (BAC-FISH) and cyclingprimed in situ labeling (C-PRINS) techniques were evaluated for integration of physical and genetic maps of sunflower (Helianthus annuus L.). Single-site SSR markers were selected from three linkage groups of a high-density sunflower genetic map. This selection was based on previously identified QTL associated to S. sclerotiorum. These markers were used to select BACs contaning single copy sequences for BAC-FISH aplication. Blocking of highly dispersed repetitive sunflower sequences reduced unspecific hybridization, and allowed the detection of specific signals for BACs containing SSR markers HA4222 and HA2600, anchored to LG 16 and LG 10, respectively. Single-site FISH signal detection was optimized by adjusting the relative quantity and quality of unlabelled repetitive sequences present in the blocking DNA. The SSR marker ORS1247 anchored to the LG 17 was detected by C-PRINS, which yielded fluorescence signals that were specific and intense. This progress in localizing single-copy sequences using BAC-FISH and indirect CPRINS strategies in sunflower will facilitate the integration of genetic and physical maps, allowing the identification of chromosomes containing key genes and/or QTL associated to agronomic important traits in sunflower. |
| format |
JOUR |
| author |
Talia, P. Greizerstein, E.J. Hopp, H.E. Paniego, N. Poggio, L. Heinz, R.A. |
| author_facet |
Talia, P. Greizerstein, E.J. Hopp, H.E. Paniego, N. Poggio, L. Heinz, R.A. |
| author_sort |
Talia, P. |
| title |
Detection of single copy sequences using BAC-FISH and C-PRINS techniques in sunflower chromosomes |
| title_short |
Detection of single copy sequences using BAC-FISH and C-PRINS techniques in sunflower chromosomes |
| title_full |
Detection of single copy sequences using BAC-FISH and C-PRINS techniques in sunflower chromosomes |
| title_fullStr |
Detection of single copy sequences using BAC-FISH and C-PRINS techniques in sunflower chromosomes |
| title_full_unstemmed |
Detection of single copy sequences using BAC-FISH and C-PRINS techniques in sunflower chromosomes |
| title_sort |
detection of single copy sequences using bac-fish and c-prins techniques in sunflower chromosomes |
| url |
http://hdl.handle.net/20.500.12110/paper_03279545_v35_n1_p19_Talia |
| work_keys_str_mv |
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| _version_ |
1807318893032636416 |