Glucocorticoid regulation of in vitro astrocyte phagocytosis

Astrocytes participate in central nervous system injury, degenerative diseases and also perform macrophagic functions. The present work investigates: 1) the effect of the physiological glucocorticoid corticosterone (CORT) and the synthetic agonist dexamethasone (DEX) on latex beads phagocytosis by n...

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Autores principales:	Roldán, A., Gogg, S., Ferrini, M., Schillaci, R., De Nicola, A.F.
Formato:	JOUR
Materias:	Astrocytes phagocytosis Corticosterone Dexamethasone Glucocorticoid receptors antiinflammatory agent corticosterone dexamethasone glucocorticoid glucocorticoid receptor animal article astrocyte brain cortex cell culture chemistry cytology drug effect immunology metabolism phagocytosis physiology rat Sprague Dawley rat Animals Anti-Inflammatory Agents Astrocytes Cells, Cultured Cerebral Cortex Glucocorticoids Phagocytosis Rats Rats, Sprague-Dawley Receptors, Glucocorticoid
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_03279545_v21_n1_p83_Roldan
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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spelling	todo:paper_03279545_v21_n1_p83_Roldan2023-10-03T15:25:07Z Glucocorticoid regulation of in vitro astrocyte phagocytosis Roldán, A. Gogg, S. Ferrini, M. Schillaci, R. De Nicola, A.F. Astrocytes phagocytosis Corticosterone Dexamethasone Glucocorticoid receptors antiinflammatory agent corticosterone dexamethasone glucocorticoid glucocorticoid receptor animal article astrocyte brain cortex cell culture chemistry cytology drug effect immunology metabolism phagocytosis physiology rat Sprague Dawley rat Animals Anti-Inflammatory Agents Astrocytes Cells, Cultured Cerebral Cortex Corticosterone Dexamethasone Glucocorticoids Phagocytosis Rats Rats, Sprague-Dawley Receptors, Glucocorticoid Astrocytes participate in central nervous system injury, degenerative diseases and also perform macrophagic functions. The present work investigates: 1) the effect of the physiological glucocorticoid corticosterone (CORT) and the synthetic agonist dexamethasone (DEX) on latex beads phagocytosis by neonatal rat cortical astrocytes in culture, and 2) the expression of immunoreactive glucocorticoid receptors (GR) in astrocytes cultured in different media with or without a pulse application of CORT. The results indicated that glucocorticoids reduced astrocyte phagocytic activity, as occurred with macrophages, independently of the culturing conditions employed. The extent of phagocytosis was inversely related to nuclear immunostaining for GR in cultures in fetal calf serum, which contained endogenous glucocorticoid. However, no correlation was found between nuclear GR and phagocytosis for cultures in glucocorticoid-free medium or in medium containing CORT. It is suggested that additional factors, besides the GR, may be involved in glucocorticoid modulation of astrocyte phagocytosis. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_03279545_v21_n1_p83_Roldan
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Astrocytes phagocytosis Corticosterone Dexamethasone Glucocorticoid receptors antiinflammatory agent corticosterone dexamethasone glucocorticoid glucocorticoid receptor animal article astrocyte brain cortex cell culture chemistry cytology drug effect immunology metabolism phagocytosis physiology rat Sprague Dawley rat Animals Anti-Inflammatory Agents Astrocytes Cells, Cultured Cerebral Cortex Corticosterone Dexamethasone Glucocorticoids Phagocytosis Rats Rats, Sprague-Dawley Receptors, Glucocorticoid
spellingShingle	Astrocytes phagocytosis Corticosterone Dexamethasone Glucocorticoid receptors antiinflammatory agent corticosterone dexamethasone glucocorticoid glucocorticoid receptor animal article astrocyte brain cortex cell culture chemistry cytology drug effect immunology metabolism phagocytosis physiology rat Sprague Dawley rat Animals Anti-Inflammatory Agents Astrocytes Cells, Cultured Cerebral Cortex Corticosterone Dexamethasone Glucocorticoids Phagocytosis Rats Rats, Sprague-Dawley Receptors, Glucocorticoid Roldán, A. Gogg, S. Ferrini, M. Schillaci, R. De Nicola, A.F. Glucocorticoid regulation of in vitro astrocyte phagocytosis
topic_facet	Astrocytes phagocytosis Corticosterone Dexamethasone Glucocorticoid receptors antiinflammatory agent corticosterone dexamethasone glucocorticoid glucocorticoid receptor animal article astrocyte brain cortex cell culture chemistry cytology drug effect immunology metabolism phagocytosis physiology rat Sprague Dawley rat Animals Anti-Inflammatory Agents Astrocytes Cells, Cultured Cerebral Cortex Corticosterone Dexamethasone Glucocorticoids Phagocytosis Rats Rats, Sprague-Dawley Receptors, Glucocorticoid
description	Astrocytes participate in central nervous system injury, degenerative diseases and also perform macrophagic functions. The present work investigates: 1) the effect of the physiological glucocorticoid corticosterone (CORT) and the synthetic agonist dexamethasone (DEX) on latex beads phagocytosis by neonatal rat cortical astrocytes in culture, and 2) the expression of immunoreactive glucocorticoid receptors (GR) in astrocytes cultured in different media with or without a pulse application of CORT. The results indicated that glucocorticoids reduced astrocyte phagocytic activity, as occurred with macrophages, independently of the culturing conditions employed. The extent of phagocytosis was inversely related to nuclear immunostaining for GR in cultures in fetal calf serum, which contained endogenous glucocorticoid. However, no correlation was found between nuclear GR and phagocytosis for cultures in glucocorticoid-free medium or in medium containing CORT. It is suggested that additional factors, besides the GR, may be involved in glucocorticoid modulation of astrocyte phagocytosis.
format	JOUR
author	Roldán, A. Gogg, S. Ferrini, M. Schillaci, R. De Nicola, A.F.
author_facet	Roldán, A. Gogg, S. Ferrini, M. Schillaci, R. De Nicola, A.F.
author_sort	Roldán, A.
title	Glucocorticoid regulation of in vitro astrocyte phagocytosis
title_short	Glucocorticoid regulation of in vitro astrocyte phagocytosis
title_full	Glucocorticoid regulation of in vitro astrocyte phagocytosis
title_fullStr	Glucocorticoid regulation of in vitro astrocyte phagocytosis
title_full_unstemmed	Glucocorticoid regulation of in vitro astrocyte phagocytosis
title_sort	glucocorticoid regulation of in vitro astrocyte phagocytosis
url	http://hdl.handle.net/20.500.12110/paper_03279545_v21_n1_p83_Roldan
work_keys_str_mv	AT roldana glucocorticoidregulationofinvitroastrocytephagocytosis AT goggs glucocorticoidregulationofinvitroastrocytephagocytosis AT ferrinim glucocorticoidregulationofinvitroastrocytephagocytosis AT schillacir glucocorticoidregulationofinvitroastrocytephagocytosis AT denicolaaf glucocorticoidregulationofinvitroastrocytephagocytosis
_version_	1807323122625413120

Glucocorticoid regulation of in vitro astrocyte phagocytosis

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