Detection of endothelial cells by MEC 13.3 monoclonal antibody in mice mammary tumors

In order to clearly visualize blood vessels, the monoclonal antibody (mAb) MEC 13.3 was used for an immunohistochemical staining on frozen sections of different mice mammary tumors. MEC 13.3 mAb is specific for endothelial cells (ECs) of mouse blood vessels and recognizes a molecule related to the m...

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Autores principales:	Vanzulli, S., Gazzaniga, S., Braidot, M.F., Vecchi, A., Mantovani, A., Wainstok De Calmanovici, R.
Formato:	JOUR
Materias:	Endothelial cells MEC 13.3 Mice mammary tumors Mouse CD31/PECAM monoclonal antibody adenocarcinoma animal antibody specificity antigen antibody reaction article Bagg albino mouse cell culture chemistry cytology experimental neoplasm female immunohistochemistry immunology microcirculation mouse udder vascular endothelium vascularization Adenocarcinoma Animals Antibodies, Monoclonal Antibody Specificity Antigen-Antibody Reactions Endothelium, Vascular Female Immunohistochemistry Mammary Glands, Animal Mammary Neoplasms, Experimental Mice Mice, Inbred BALB C Microcirculation Tumor Cells, Cultured
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_03279545_v21_n1_p39_Vanzulli
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_03279545_v21_n1_p39_Vanzulli
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spelling	todo:paper_03279545_v21_n1_p39_Vanzulli2023-10-03T15:25:07Z Detection of endothelial cells by MEC 13.3 monoclonal antibody in mice mammary tumors Vanzulli, S. Gazzaniga, S. Braidot, M.F. Vecchi, A. Mantovani, A. Wainstok De Calmanovici, R. Endothelial cells MEC 13.3 Mice mammary tumors Mouse CD31/PECAM monoclonal antibody adenocarcinoma animal antibody specificity antigen antibody reaction article Bagg albino mouse cell culture chemistry cytology experimental neoplasm female immunohistochemistry immunology microcirculation mouse udder vascular endothelium vascularization Adenocarcinoma Animals Antibodies, Monoclonal Antibody Specificity Antigen-Antibody Reactions Endothelium, Vascular Female Immunohistochemistry Mammary Glands, Animal Mammary Neoplasms, Experimental Mice Mice, Inbred BALB C Microcirculation Tumor Cells, Cultured In order to clearly visualize blood vessels, the monoclonal antibody (mAb) MEC 13.3 was used for an immunohistochemical staining on frozen sections of different mice mammary tumors. MEC 13.3 mAb is specific for endothelial cells (ECs) of mouse blood vessels and recognizes a molecule related to the murine form of CD31/PECAM. This mAb with immunoenzymatic technique or inmunofluorescent labelling, was found to be a useful tool to quantify tumor neovascularization. Specifically, membrane reinforcement could be observed in vessel ECs, indicating the expression of CD31/ PECAM in their surface. The staining of ECs from tumors and from normal tissues was also compared. In this work, the use of MEC13.3 mAb is reported to recognize mice mammary tumor ECs as a useful tool to identify neovascularization. It would also be helpful for research on the origin and function of vascular endothelium in murine tumor experimental models. Fil:Gazzaniga, S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Wainstok De Calmanovici, R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_03279545_v21_n1_p39_Vanzulli
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Endothelial cells MEC 13.3 Mice mammary tumors Mouse CD31/PECAM monoclonal antibody adenocarcinoma animal antibody specificity antigen antibody reaction article Bagg albino mouse cell culture chemistry cytology experimental neoplasm female immunohistochemistry immunology microcirculation mouse udder vascular endothelium vascularization Adenocarcinoma Animals Antibodies, Monoclonal Antibody Specificity Antigen-Antibody Reactions Endothelium, Vascular Female Immunohistochemistry Mammary Glands, Animal Mammary Neoplasms, Experimental Mice Mice, Inbred BALB C Microcirculation Tumor Cells, Cultured
spellingShingle	Endothelial cells MEC 13.3 Mice mammary tumors Mouse CD31/PECAM monoclonal antibody adenocarcinoma animal antibody specificity antigen antibody reaction article Bagg albino mouse cell culture chemistry cytology experimental neoplasm female immunohistochemistry immunology microcirculation mouse udder vascular endothelium vascularization Adenocarcinoma Animals Antibodies, Monoclonal Antibody Specificity Antigen-Antibody Reactions Endothelium, Vascular Female Immunohistochemistry Mammary Glands, Animal Mammary Neoplasms, Experimental Mice Mice, Inbred BALB C Microcirculation Tumor Cells, Cultured Vanzulli, S. Gazzaniga, S. Braidot, M.F. Vecchi, A. Mantovani, A. Wainstok De Calmanovici, R. Detection of endothelial cells by MEC 13.3 monoclonal antibody in mice mammary tumors
topic_facet	Endothelial cells MEC 13.3 Mice mammary tumors Mouse CD31/PECAM monoclonal antibody adenocarcinoma animal antibody specificity antigen antibody reaction article Bagg albino mouse cell culture chemistry cytology experimental neoplasm female immunohistochemistry immunology microcirculation mouse udder vascular endothelium vascularization Adenocarcinoma Animals Antibodies, Monoclonal Antibody Specificity Antigen-Antibody Reactions Endothelium, Vascular Female Immunohistochemistry Mammary Glands, Animal Mammary Neoplasms, Experimental Mice Mice, Inbred BALB C Microcirculation Tumor Cells, Cultured
description	In order to clearly visualize blood vessels, the monoclonal antibody (mAb) MEC 13.3 was used for an immunohistochemical staining on frozen sections of different mice mammary tumors. MEC 13.3 mAb is specific for endothelial cells (ECs) of mouse blood vessels and recognizes a molecule related to the murine form of CD31/PECAM. This mAb with immunoenzymatic technique or inmunofluorescent labelling, was found to be a useful tool to quantify tumor neovascularization. Specifically, membrane reinforcement could be observed in vessel ECs, indicating the expression of CD31/ PECAM in their surface. The staining of ECs from tumors and from normal tissues was also compared. In this work, the use of MEC13.3 mAb is reported to recognize mice mammary tumor ECs as a useful tool to identify neovascularization. It would also be helpful for research on the origin and function of vascular endothelium in murine tumor experimental models.
format	JOUR
author	Vanzulli, S. Gazzaniga, S. Braidot, M.F. Vecchi, A. Mantovani, A. Wainstok De Calmanovici, R.
author_facet	Vanzulli, S. Gazzaniga, S. Braidot, M.F. Vecchi, A. Mantovani, A. Wainstok De Calmanovici, R.
author_sort	Vanzulli, S.
title	Detection of endothelial cells by MEC 13.3 monoclonal antibody in mice mammary tumors
title_short	Detection of endothelial cells by MEC 13.3 monoclonal antibody in mice mammary tumors
title_full	Detection of endothelial cells by MEC 13.3 monoclonal antibody in mice mammary tumors
title_fullStr	Detection of endothelial cells by MEC 13.3 monoclonal antibody in mice mammary tumors
title_full_unstemmed	Detection of endothelial cells by MEC 13.3 monoclonal antibody in mice mammary tumors
title_sort	detection of endothelial cells by mec 13.3 monoclonal antibody in mice mammary tumors
url	http://hdl.handle.net/20.500.12110/paper_03279545_v21_n1_p39_Vanzulli
work_keys_str_mv	AT vanzullis detectionofendothelialcellsbymec133monoclonalantibodyinmicemammarytumors AT gazzanigas detectionofendothelialcellsbymec133monoclonalantibodyinmicemammarytumors AT braidotmf detectionofendothelialcellsbymec133monoclonalantibodyinmicemammarytumors AT vecchia detectionofendothelialcellsbymec133monoclonalantibodyinmicemammarytumors AT mantovania detectionofendothelialcellsbymec133monoclonalantibodyinmicemammarytumors AT wainstokdecalmanovicir detectionofendothelialcellsbymec133monoclonalantibodyinmicemammarytumors
_version_	1782027339608096768

Detection of endothelial cells by MEC 13.3 monoclonal antibody in mice mammary tumors

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