Detection of manganese peroxidase and other exoenzymes in four isolates of Geastrum (Geastrales) in pure culture

Knowledge regarding the enzymatic machinery of fungi is decisive to understand their ecological role. The species of the genus Geastrum are known to grow extremely slowly in pure culture, which makes it difficult to evaluate physiological parameters such as enzyme activity. Qualitative assays were p...

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Autores principales:	Kuhar, F., Castiglia, V.C., Zamora, J.C.
Formato:	JOUR
Materias:	Cellulase Laccase Lipase Litter degradation Xylanase amino acid amylase cellulase glycosidase laccase manganese peroxidase nitrophenol pectin polygalacturonase proton transporting adenosine triphosphate synthase ribosome DNA RNA polymerase II triacylglycerol lipase xenobiotic agent xylan 1,4 beta xylosidase xylan endo 1,3 beta xylosidase carbon culture medium fungal protein peroxidase agar medium Article Cantharellales decolorization enzyme activity fungal cell culture Geastrum Geastrum argentinum Geastrum morganii Geastrum papinuttii Geastrum plicatum litter decomposition mycelium nonhuman Basidiomycetes comparative study enzymology growth, development and aging metabolism species difference Amylases Basidiomycota Carbon Culture Media Fungal Proteins Peroxidases Polygalacturonase Species Specificity
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_03257541_v48_n4_p274_Kuhar
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_03257541_v48_n4_p274_Kuhar
record_format	dspace
spelling	todo:paper_03257541_v48_n4_p274_Kuhar2023-10-03T15:24:23Z Detection of manganese peroxidase and other exoenzymes in four isolates of Geastrum (Geastrales) in pure culture Kuhar, F. Castiglia, V.C. Zamora, J.C. Cellulase Laccase Lipase Litter degradation Xylanase amino acid amylase cellulase glycosidase laccase manganese peroxidase nitrophenol pectin polygalacturonase proton transporting adenosine triphosphate synthase ribosome DNA RNA polymerase II triacylglycerol lipase xenobiotic agent xylan 1,4 beta xylosidase xylan endo 1,3 beta xylosidase amylase carbon cellulase culture medium fungal protein laccase manganese peroxidase peroxidase polygalacturonase triacylglycerol lipase agar medium Article Cantharellales decolorization enzyme activity fungal cell culture Geastrum Geastrum argentinum Geastrum morganii Geastrum papinuttii Geastrum plicatum litter decomposition mycelium nonhuman Basidiomycetes comparative study culture medium enzymology growth, development and aging metabolism species difference Amylases Basidiomycota Carbon Cellulase Culture Media Fungal Proteins Laccase Lipase Peroxidases Polygalacturonase Species Specificity Knowledge regarding the enzymatic machinery of fungi is decisive to understand their ecological role. The species of the genus Geastrum are known to grow extremely slowly in pure culture, which makes it difficult to evaluate physiological parameters such as enzyme activity. Qualitative assays were performed on isolates of four species of this genus, showing evidence of laccase, cellulase, pectinase, amylase and lipase activity and suggesting that a wide range of carbon sources can be exploited by these species. For the first time in this genus, quantitative assays verified manganese peroxidase activity (up to 0.6 mU/g) in 30-day old cultures, as well as laccase, β-glycosidase and β-xylosidase activities. © 2016 Asociación Argentina de Microbiología Fil:Castiglia, V.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_03257541_v48_n4_p274_Kuhar
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Cellulase Laccase Lipase Litter degradation Xylanase amino acid amylase cellulase glycosidase laccase manganese peroxidase nitrophenol pectin polygalacturonase proton transporting adenosine triphosphate synthase ribosome DNA RNA polymerase II triacylglycerol lipase xenobiotic agent xylan 1,4 beta xylosidase xylan endo 1,3 beta xylosidase amylase carbon cellulase culture medium fungal protein laccase manganese peroxidase peroxidase polygalacturonase triacylglycerol lipase agar medium Article Cantharellales decolorization enzyme activity fungal cell culture Geastrum Geastrum argentinum Geastrum morganii Geastrum papinuttii Geastrum plicatum litter decomposition mycelium nonhuman Basidiomycetes comparative study culture medium enzymology growth, development and aging metabolism species difference Amylases Basidiomycota Carbon Cellulase Culture Media Fungal Proteins Laccase Lipase Peroxidases Polygalacturonase Species Specificity
spellingShingle	Cellulase Laccase Lipase Litter degradation Xylanase amino acid amylase cellulase glycosidase laccase manganese peroxidase nitrophenol pectin polygalacturonase proton transporting adenosine triphosphate synthase ribosome DNA RNA polymerase II triacylglycerol lipase xenobiotic agent xylan 1,4 beta xylosidase xylan endo 1,3 beta xylosidase amylase carbon cellulase culture medium fungal protein laccase manganese peroxidase peroxidase polygalacturonase triacylglycerol lipase agar medium Article Cantharellales decolorization enzyme activity fungal cell culture Geastrum Geastrum argentinum Geastrum morganii Geastrum papinuttii Geastrum plicatum litter decomposition mycelium nonhuman Basidiomycetes comparative study culture medium enzymology growth, development and aging metabolism species difference Amylases Basidiomycota Carbon Cellulase Culture Media Fungal Proteins Laccase Lipase Peroxidases Polygalacturonase Species Specificity Kuhar, F. Castiglia, V.C. Zamora, J.C. Detection of manganese peroxidase and other exoenzymes in four isolates of Geastrum (Geastrales) in pure culture
topic_facet	Cellulase Laccase Lipase Litter degradation Xylanase amino acid amylase cellulase glycosidase laccase manganese peroxidase nitrophenol pectin polygalacturonase proton transporting adenosine triphosphate synthase ribosome DNA RNA polymerase II triacylglycerol lipase xenobiotic agent xylan 1,4 beta xylosidase xylan endo 1,3 beta xylosidase amylase carbon cellulase culture medium fungal protein laccase manganese peroxidase peroxidase polygalacturonase triacylglycerol lipase agar medium Article Cantharellales decolorization enzyme activity fungal cell culture Geastrum Geastrum argentinum Geastrum morganii Geastrum papinuttii Geastrum plicatum litter decomposition mycelium nonhuman Basidiomycetes comparative study culture medium enzymology growth, development and aging metabolism species difference Amylases Basidiomycota Carbon Cellulase Culture Media Fungal Proteins Laccase Lipase Peroxidases Polygalacturonase Species Specificity
description	Knowledge regarding the enzymatic machinery of fungi is decisive to understand their ecological role. The species of the genus Geastrum are known to grow extremely slowly in pure culture, which makes it difficult to evaluate physiological parameters such as enzyme activity. Qualitative assays were performed on isolates of four species of this genus, showing evidence of laccase, cellulase, pectinase, amylase and lipase activity and suggesting that a wide range of carbon sources can be exploited by these species. For the first time in this genus, quantitative assays verified manganese peroxidase activity (up to 0.6 mU/g) in 30-day old cultures, as well as laccase, β-glycosidase and β-xylosidase activities. © 2016 Asociación Argentina de Microbiología
format	JOUR
author	Kuhar, F. Castiglia, V.C. Zamora, J.C.
author_facet	Kuhar, F. Castiglia, V.C. Zamora, J.C.
author_sort	Kuhar, F.
title	Detection of manganese peroxidase and other exoenzymes in four isolates of Geastrum (Geastrales) in pure culture
title_short	Detection of manganese peroxidase and other exoenzymes in four isolates of Geastrum (Geastrales) in pure culture
title_full	Detection of manganese peroxidase and other exoenzymes in four isolates of Geastrum (Geastrales) in pure culture
title_fullStr	Detection of manganese peroxidase and other exoenzymes in four isolates of Geastrum (Geastrales) in pure culture
title_full_unstemmed	Detection of manganese peroxidase and other exoenzymes in four isolates of Geastrum (Geastrales) in pure culture
title_sort	detection of manganese peroxidase and other exoenzymes in four isolates of geastrum (geastrales) in pure culture
url	http://hdl.handle.net/20.500.12110/paper_03257541_v48_n4_p274_Kuhar
work_keys_str_mv	AT kuharf detectionofmanganeseperoxidaseandotherexoenzymesinfourisolatesofgeastrumgeastralesinpureculture AT castigliavc detectionofmanganeseperoxidaseandotherexoenzymesinfourisolatesofgeastrumgeastralesinpureculture AT zamorajc detectionofmanganeseperoxidaseandotherexoenzymesinfourisolatesofgeastrumgeastralesinpureculture
_version_	1782025881306267648

Detection of manganese peroxidase and other exoenzymes in four isolates of Geastrum (Geastrales) in pure culture

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