Multiple-locus variable-number tandem repeat analysis of reference strains used for the diagnosis of leptospirosis in Argentina
Leptospirosis is a worldwide zoonosis caused by a spirochete that belongs to the genus Leptospira. In the last years, new methods, such as the PCR-based multiple-locus variable-number tandem repeat analysis (MLVA), have been developed for the genotyping of leptospires. In the present work, the MLVA...
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todo:paper_03257541_v43_n4_p251_Pavan2023-10-03T15:24:19Z Multiple-locus variable-number tandem repeat analysis of reference strains used for the diagnosis of leptospirosis in Argentina Pavan, M.E. Brihuega, B. Pettinari, M.J. Cairó, F. Genotyping Leptospira borgpetersenii Leptospira interrogans Leptospira kirschneri MLVA Reference strains VNTR agar gel electrophoresis Argentina article bacterial strain bacterium identification genotype intermethod comparison Leptospira borgpetersenii Leptospira interrogans Leptospira kirschneri leptospirosis multiple locus variable number tandem repeat analysis nonhuman variable number of tandem repeat Animals Animals, Domestic Animals, Wild Argentina DNA, Bacterial Electrophoresis, Agar Gel Genotype Humans Leptospira Leptospira interrogans Leptospirosis Minisatellite Repeats Reference Standards Species Specificity Leptospirosis is a worldwide zoonosis caused by a spirochete that belongs to the genus Leptospira. In the last years, new methods, such as the PCR-based multiple-locus variable-number tandem repeat analysis (MLVA), have been developed for the genotyping of leptospires. In the present work, the MLVA patterns for all reference strains used in Argentina for bovine, ovine, porcine, equine, caprine and canine leptospirosis diagnosis, as well as in human and wild animal diagnosis, were obtained. MLVA results are presented in such a way that they can be readily used for the identification of these strains by the simple and direct comparison of agarose gels. Making the use and interpretation of the MLVA for leptospires typing easier will help increase the use of this method as a routine procedure for human and animal diagnosis, for epidemiological studies, vaccine control and other applications. Fil:Pettinari, M.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_03257541_v43_n4_p251_Pavan |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Genotyping Leptospira borgpetersenii Leptospira interrogans Leptospira kirschneri MLVA Reference strains VNTR agar gel electrophoresis Argentina article bacterial strain bacterium identification genotype intermethod comparison Leptospira borgpetersenii Leptospira interrogans Leptospira kirschneri leptospirosis multiple locus variable number tandem repeat analysis nonhuman variable number of tandem repeat Animals Animals, Domestic Animals, Wild Argentina DNA, Bacterial Electrophoresis, Agar Gel Genotype Humans Leptospira Leptospira interrogans Leptospirosis Minisatellite Repeats Reference Standards Species Specificity |
| spellingShingle |
Genotyping Leptospira borgpetersenii Leptospira interrogans Leptospira kirschneri MLVA Reference strains VNTR agar gel electrophoresis Argentina article bacterial strain bacterium identification genotype intermethod comparison Leptospira borgpetersenii Leptospira interrogans Leptospira kirschneri leptospirosis multiple locus variable number tandem repeat analysis nonhuman variable number of tandem repeat Animals Animals, Domestic Animals, Wild Argentina DNA, Bacterial Electrophoresis, Agar Gel Genotype Humans Leptospira Leptospira interrogans Leptospirosis Minisatellite Repeats Reference Standards Species Specificity Pavan, M.E. Brihuega, B. Pettinari, M.J. Cairó, F. Multiple-locus variable-number tandem repeat analysis of reference strains used for the diagnosis of leptospirosis in Argentina |
| topic_facet |
Genotyping Leptospira borgpetersenii Leptospira interrogans Leptospira kirschneri MLVA Reference strains VNTR agar gel electrophoresis Argentina article bacterial strain bacterium identification genotype intermethod comparison Leptospira borgpetersenii Leptospira interrogans Leptospira kirschneri leptospirosis multiple locus variable number tandem repeat analysis nonhuman variable number of tandem repeat Animals Animals, Domestic Animals, Wild Argentina DNA, Bacterial Electrophoresis, Agar Gel Genotype Humans Leptospira Leptospira interrogans Leptospirosis Minisatellite Repeats Reference Standards Species Specificity |
| description |
Leptospirosis is a worldwide zoonosis caused by a spirochete that belongs to the genus Leptospira. In the last years, new methods, such as the PCR-based multiple-locus variable-number tandem repeat analysis (MLVA), have been developed for the genotyping of leptospires. In the present work, the MLVA patterns for all reference strains used in Argentina for bovine, ovine, porcine, equine, caprine and canine leptospirosis diagnosis, as well as in human and wild animal diagnosis, were obtained. MLVA results are presented in such a way that they can be readily used for the identification of these strains by the simple and direct comparison of agarose gels. Making the use and interpretation of the MLVA for leptospires typing easier will help increase the use of this method as a routine procedure for human and animal diagnosis, for epidemiological studies, vaccine control and other applications. |
| format |
JOUR |
| author |
Pavan, M.E. Brihuega, B. Pettinari, M.J. Cairó, F. |
| author_facet |
Pavan, M.E. Brihuega, B. Pettinari, M.J. Cairó, F. |
| author_sort |
Pavan, M.E. |
| title |
Multiple-locus variable-number tandem repeat analysis of reference strains used for the diagnosis of leptospirosis in Argentina |
| title_short |
Multiple-locus variable-number tandem repeat analysis of reference strains used for the diagnosis of leptospirosis in Argentina |
| title_full |
Multiple-locus variable-number tandem repeat analysis of reference strains used for the diagnosis of leptospirosis in Argentina |
| title_fullStr |
Multiple-locus variable-number tandem repeat analysis of reference strains used for the diagnosis of leptospirosis in Argentina |
| title_full_unstemmed |
Multiple-locus variable-number tandem repeat analysis of reference strains used for the diagnosis of leptospirosis in Argentina |
| title_sort |
multiple-locus variable-number tandem repeat analysis of reference strains used for the diagnosis of leptospirosis in argentina |
| url |
http://hdl.handle.net/20.500.12110/paper_03257541_v43_n4_p251_Pavan |
| work_keys_str_mv |
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| _version_ |
1782029232454500352 |