Extraction and characterization of polygalacturonase of fomes sclerodermeus produced by solid-state fermentation
Polygalacturonase (PG) production by Fomes sclerodermeus using solid-state fermentation (SSF) was carried out. Maximal PG activity (26 U/gdw) was obtained between days 11 and 13 at the end of exponential growth. PG activity in the crude extract was more stable at pH 5-6 and 30 °C and had optimum act...
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_03257541_v42_n1_p57_Salariato |
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todo:paper_03257541_v42_n1_p57_Salariato2023-10-03T15:24:17Z Extraction and characterization of polygalacturonase of fomes sclerodermeus produced by solid-state fermentation Salariato, D. Diorio, L.A. Mouso, N. Forchiassin, F. Fomes sclerodermeus Polygalacturonase Solid state fermentation polygalacturonase sodium sulfate article enzyme synthesis Fomes sclerodermeus fungus growth nonhuman solid state fermentation Cell Fractionation Coriolaceae Fermentation Fungal Proteins Hydrogen-Ion Concentration Mycology Polygalacturonase Solvents Temperature Polygalacturonase (PG) production by Fomes sclerodermeus using solid-state fermentation (SSF) was carried out. Maximal PG activity (26 U/gdw) was obtained between days 11 and 13 at the end of exponential growth. PG activity in the crude extract was more stable at pH 5-6 and 30 °C and had optimum activity at pH 5 and 50 °C. Optimal conditions for PG extraction were: one time extraction with Na2SO4 as solvent with 10 min. of agitation. In a scale-up system, PG activity per gram of dry substrate decreased about 60% compared with the activity obtained in an Erlenmeyer flask; however, high total PG activity was obtained. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_03257541_v42_n1_p57_Salariato |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Fomes sclerodermeus Polygalacturonase Solid state fermentation polygalacturonase sodium sulfate article enzyme synthesis Fomes sclerodermeus fungus growth nonhuman solid state fermentation Cell Fractionation Coriolaceae Fermentation Fungal Proteins Hydrogen-Ion Concentration Mycology Polygalacturonase Solvents Temperature |
| spellingShingle |
Fomes sclerodermeus Polygalacturonase Solid state fermentation polygalacturonase sodium sulfate article enzyme synthesis Fomes sclerodermeus fungus growth nonhuman solid state fermentation Cell Fractionation Coriolaceae Fermentation Fungal Proteins Hydrogen-Ion Concentration Mycology Polygalacturonase Solvents Temperature Salariato, D. Diorio, L.A. Mouso, N. Forchiassin, F. Extraction and characterization of polygalacturonase of fomes sclerodermeus produced by solid-state fermentation |
| topic_facet |
Fomes sclerodermeus Polygalacturonase Solid state fermentation polygalacturonase sodium sulfate article enzyme synthesis Fomes sclerodermeus fungus growth nonhuman solid state fermentation Cell Fractionation Coriolaceae Fermentation Fungal Proteins Hydrogen-Ion Concentration Mycology Polygalacturonase Solvents Temperature |
| description |
Polygalacturonase (PG) production by Fomes sclerodermeus using solid-state fermentation (SSF) was carried out. Maximal PG activity (26 U/gdw) was obtained between days 11 and 13 at the end of exponential growth. PG activity in the crude extract was more stable at pH 5-6 and 30 °C and had optimum activity at pH 5 and 50 °C. Optimal conditions for PG extraction were: one time extraction with Na2SO4 as solvent with 10 min. of agitation. In a scale-up system, PG activity per gram of dry substrate decreased about 60% compared with the activity obtained in an Erlenmeyer flask; however, high total PG activity was obtained. |
| format |
JOUR |
| author |
Salariato, D. Diorio, L.A. Mouso, N. Forchiassin, F. |
| author_facet |
Salariato, D. Diorio, L.A. Mouso, N. Forchiassin, F. |
| author_sort |
Salariato, D. |
| title |
Extraction and characterization of polygalacturonase of fomes sclerodermeus produced by solid-state fermentation |
| title_short |
Extraction and characterization of polygalacturonase of fomes sclerodermeus produced by solid-state fermentation |
| title_full |
Extraction and characterization of polygalacturonase of fomes sclerodermeus produced by solid-state fermentation |
| title_fullStr |
Extraction and characterization of polygalacturonase of fomes sclerodermeus produced by solid-state fermentation |
| title_full_unstemmed |
Extraction and characterization of polygalacturonase of fomes sclerodermeus produced by solid-state fermentation |
| title_sort |
extraction and characterization of polygalacturonase of fomes sclerodermeus produced by solid-state fermentation |
| url |
http://hdl.handle.net/20.500.12110/paper_03257541_v42_n1_p57_Salariato |
| work_keys_str_mv |
AT salariatod extractionandcharacterizationofpolygalacturonaseoffomessclerodermeusproducedbysolidstatefermentation AT dioriola extractionandcharacterizationofpolygalacturonaseoffomessclerodermeusproducedbysolidstatefermentation AT mouson extractionandcharacterizationofpolygalacturonaseoffomessclerodermeusproducedbysolidstatefermentation AT forchiassinf extractionandcharacterizationofpolygalacturonaseoffomessclerodermeusproducedbysolidstatefermentation |
| _version_ |
1782030707402473472 |