Diagnosis of caseous lymphadenitis in sheep from Patagonia
Caseous lymphadenitis (CLA) is a chronic bacterial, infectious and contagious disease caused by Corynebacterium pseudotuberculosis. It affects sheep and results in abscesses of the lymph nodes in subcutaneous tissue, as well as in internal organs such as lungs, liver and kidneys. Differential diagno...
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todo:paper_03257541_v39_n1_p44_EstevaoBelchior2023-10-03T15:24:17Z Diagnosis of caseous lymphadenitis in sheep from Patagonia Estevao Belchior, S. Gallardo, A. Ábalos, A. Díaz, Y. Álvarez, L. Callejo, R. Prieto, M. Jodor, N. Jensen, O. Corynebacterium pseudotuberculosis Pseudotuberculosis Sheep DNA 16S Argentina article bacterium identification bacterium isolation cell infiltration diagnostic procedure DNA sequence epithelioid cell histopathology lymphadenitis nonhuman polymorphonuclear cell sheep tissue necrosis Animals Argentina Corynebacterium Infections Corynebacterium pseudotuberculosis Sheep Diseases Caseous lymphadenitis (CLA) is a chronic bacterial, infectious and contagious disease caused by Corynebacterium pseudotuberculosis. It affects sheep and results in abscesses of the lymph nodes in subcutaneous tissue, as well as in internal organs such as lungs, liver and kidneys. Differential diagnosis of the disease is based on the isolation and biochemical identification of the etiological agent. The purpose of this study was to characterize the bacteria isolated from typical CLA lesions in sheep from Patagonia, Argentina, at metabolic and genetic levels. Macroscopic observations show a fibrous membrane containing caseous necrotic tissue. Histopathological analysis shows an eosinophilic necrotic area surrounded by epitheloid cells and polymorphonuclear infiltration. Other analyses performed such as microscopic observations, in vitro culture, biochemical tests and 16s rDNA sequencing confirmed diagnosis of caseous lymphadenitis due to C. pseudotuberculosis. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_03257541_v39_n1_p44_EstevaoBelchior |
| institution |
Universidad de Buenos Aires |
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I-28 |
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R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Corynebacterium pseudotuberculosis Pseudotuberculosis Sheep DNA 16S Argentina article bacterium identification bacterium isolation cell infiltration diagnostic procedure DNA sequence epithelioid cell histopathology lymphadenitis nonhuman polymorphonuclear cell sheep tissue necrosis Animals Argentina Corynebacterium Infections Corynebacterium pseudotuberculosis Sheep Diseases |
| spellingShingle |
Corynebacterium pseudotuberculosis Pseudotuberculosis Sheep DNA 16S Argentina article bacterium identification bacterium isolation cell infiltration diagnostic procedure DNA sequence epithelioid cell histopathology lymphadenitis nonhuman polymorphonuclear cell sheep tissue necrosis Animals Argentina Corynebacterium Infections Corynebacterium pseudotuberculosis Sheep Diseases Estevao Belchior, S. Gallardo, A. Ábalos, A. Díaz, Y. Álvarez, L. Callejo, R. Prieto, M. Jodor, N. Jensen, O. Diagnosis of caseous lymphadenitis in sheep from Patagonia |
| topic_facet |
Corynebacterium pseudotuberculosis Pseudotuberculosis Sheep DNA 16S Argentina article bacterium identification bacterium isolation cell infiltration diagnostic procedure DNA sequence epithelioid cell histopathology lymphadenitis nonhuman polymorphonuclear cell sheep tissue necrosis Animals Argentina Corynebacterium Infections Corynebacterium pseudotuberculosis Sheep Diseases |
| description |
Caseous lymphadenitis (CLA) is a chronic bacterial, infectious and contagious disease caused by Corynebacterium pseudotuberculosis. It affects sheep and results in abscesses of the lymph nodes in subcutaneous tissue, as well as in internal organs such as lungs, liver and kidneys. Differential diagnosis of the disease is based on the isolation and biochemical identification of the etiological agent. The purpose of this study was to characterize the bacteria isolated from typical CLA lesions in sheep from Patagonia, Argentina, at metabolic and genetic levels. Macroscopic observations show a fibrous membrane containing caseous necrotic tissue. Histopathological analysis shows an eosinophilic necrotic area surrounded by epitheloid cells and polymorphonuclear infiltration. Other analyses performed such as microscopic observations, in vitro culture, biochemical tests and 16s rDNA sequencing confirmed diagnosis of caseous lymphadenitis due to C. pseudotuberculosis. |
| format |
JOUR |
| author |
Estevao Belchior, S. Gallardo, A. Ábalos, A. Díaz, Y. Álvarez, L. Callejo, R. Prieto, M. Jodor, N. Jensen, O. |
| author_facet |
Estevao Belchior, S. Gallardo, A. Ábalos, A. Díaz, Y. Álvarez, L. Callejo, R. Prieto, M. Jodor, N. Jensen, O. |
| author_sort |
Estevao Belchior, S. |
| title |
Diagnosis of caseous lymphadenitis in sheep from Patagonia |
| title_short |
Diagnosis of caseous lymphadenitis in sheep from Patagonia |
| title_full |
Diagnosis of caseous lymphadenitis in sheep from Patagonia |
| title_fullStr |
Diagnosis of caseous lymphadenitis in sheep from Patagonia |
| title_full_unstemmed |
Diagnosis of caseous lymphadenitis in sheep from Patagonia |
| title_sort |
diagnosis of caseous lymphadenitis in sheep from patagonia |
| url |
http://hdl.handle.net/20.500.12110/paper_03257541_v39_n1_p44_EstevaoBelchior |
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AT estevaobelchiors diagnosisofcaseouslymphadenitisinsheepfrompatagonia AT gallardoa diagnosisofcaseouslymphadenitisinsheepfrompatagonia AT abalosa diagnosisofcaseouslymphadenitisinsheepfrompatagonia AT diazy diagnosisofcaseouslymphadenitisinsheepfrompatagonia AT alvarezl diagnosisofcaseouslymphadenitisinsheepfrompatagonia AT callejor diagnosisofcaseouslymphadenitisinsheepfrompatagonia AT prietom diagnosisofcaseouslymphadenitisinsheepfrompatagonia AT jodorn diagnosisofcaseouslymphadenitisinsheepfrompatagonia AT jenseno diagnosisofcaseouslymphadenitisinsheepfrompatagonia |
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