Degradation of anthraquinone dyes by Trametes trogii
The ability of the white rot fungus Trametes trogii BAFC 463 (high producer of ligninolytic enzymes, especially laccase and manganese peroxidase) to degrade the dye anthraquinone blue, refractory to bacterial attack, was evaluated. Both tropho- and idiophasic T. trogii cultures in synthetic medium (...
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todo:paper_03257541_v33_n4_p223_Levin2023-10-03T15:24:14Z Degradation of anthraquinone dyes by Trametes trogii Levin, L. Jordan, A. Forchiassin, F. Viale, A. Anthraquinone dyes Dye degradation Ligninolytic fungi Trametes trogii anthraquinone laccase manganese peroxidase anthraquinone derivative coloring agent fungal protein laccase lignin oxidoreductase oxygen article bioreactor culture medium enzyme activity enzyme assay fungal metabolism fungus culture nonhuman Trametes trametes trogii Aphyllophorales bioremediation comparative study enzymology fermentation industrial waste metabolism microbiology oxidation reduction reaction pH Anthraquinones Biodegradation, Environmental Bioreactors Coloring Agents Culture Media Fermentation Fungal Proteins Hydrogen-Ion Concentration Industrial Microbiology Industrial Waste Laccase Lignin Oxidation-Reduction Oxidoreductases Oxygen Polyporales The ability of the white rot fungus Trametes trogii BAFC 463 (high producer of ligninolytic enzymes, especially laccase and manganese peroxidase) to degrade the dye anthraquinone blue, refractory to bacterial attack, was evaluated. Both tropho- and idiophasic T. trogii cultures in synthetic medium (glucose/asparagine) and complex medium (malt extract/glucose) were able to transform up to 88% dye in 4 hours. The activity of laccase, an oxygen-dependent fenoloxidase which was present at high levels in all the conditions assayed, might be related to the ability of the fungus to degrade the colorant. This is supported by the fact that in bioreactor experiences carried out at pH 4.5 the addition of anthraquinone blue caused a decrease in the levels of soluble oxygen. However, although high levels of laccase were produced at pH 7.5, the enzyme was not active, and neither dye transformation nor loss in the levels of soluble oxygen were quantified. Fil:Levin, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Forchiassin, F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Viale, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_03257541_v33_n4_p223_Levin |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Anthraquinone dyes Dye degradation Ligninolytic fungi Trametes trogii anthraquinone laccase manganese peroxidase anthraquinone derivative coloring agent fungal protein laccase lignin oxidoreductase oxygen article bioreactor culture medium enzyme activity enzyme assay fungal metabolism fungus culture nonhuman Trametes trametes trogii Aphyllophorales bioremediation comparative study enzymology fermentation industrial waste metabolism microbiology oxidation reduction reaction pH Anthraquinones Biodegradation, Environmental Bioreactors Coloring Agents Culture Media Fermentation Fungal Proteins Hydrogen-Ion Concentration Industrial Microbiology Industrial Waste Laccase Lignin Oxidation-Reduction Oxidoreductases Oxygen Polyporales |
| spellingShingle |
Anthraquinone dyes Dye degradation Ligninolytic fungi Trametes trogii anthraquinone laccase manganese peroxidase anthraquinone derivative coloring agent fungal protein laccase lignin oxidoreductase oxygen article bioreactor culture medium enzyme activity enzyme assay fungal metabolism fungus culture nonhuman Trametes trametes trogii Aphyllophorales bioremediation comparative study enzymology fermentation industrial waste metabolism microbiology oxidation reduction reaction pH Anthraquinones Biodegradation, Environmental Bioreactors Coloring Agents Culture Media Fermentation Fungal Proteins Hydrogen-Ion Concentration Industrial Microbiology Industrial Waste Laccase Lignin Oxidation-Reduction Oxidoreductases Oxygen Polyporales Levin, L. Jordan, A. Forchiassin, F. Viale, A. Degradation of anthraquinone dyes by Trametes trogii |
| topic_facet |
Anthraquinone dyes Dye degradation Ligninolytic fungi Trametes trogii anthraquinone laccase manganese peroxidase anthraquinone derivative coloring agent fungal protein laccase lignin oxidoreductase oxygen article bioreactor culture medium enzyme activity enzyme assay fungal metabolism fungus culture nonhuman Trametes trametes trogii Aphyllophorales bioremediation comparative study enzymology fermentation industrial waste metabolism microbiology oxidation reduction reaction pH Anthraquinones Biodegradation, Environmental Bioreactors Coloring Agents Culture Media Fermentation Fungal Proteins Hydrogen-Ion Concentration Industrial Microbiology Industrial Waste Laccase Lignin Oxidation-Reduction Oxidoreductases Oxygen Polyporales |
| description |
The ability of the white rot fungus Trametes trogii BAFC 463 (high producer of ligninolytic enzymes, especially laccase and manganese peroxidase) to degrade the dye anthraquinone blue, refractory to bacterial attack, was evaluated. Both tropho- and idiophasic T. trogii cultures in synthetic medium (glucose/asparagine) and complex medium (malt extract/glucose) were able to transform up to 88% dye in 4 hours. The activity of laccase, an oxygen-dependent fenoloxidase which was present at high levels in all the conditions assayed, might be related to the ability of the fungus to degrade the colorant. This is supported by the fact that in bioreactor experiences carried out at pH 4.5 the addition of anthraquinone blue caused a decrease in the levels of soluble oxygen. However, although high levels of laccase were produced at pH 7.5, the enzyme was not active, and neither dye transformation nor loss in the levels of soluble oxygen were quantified. |
| format |
JOUR |
| author |
Levin, L. Jordan, A. Forchiassin, F. Viale, A. |
| author_facet |
Levin, L. Jordan, A. Forchiassin, F. Viale, A. |
| author_sort |
Levin, L. |
| title |
Degradation of anthraquinone dyes by Trametes trogii |
| title_short |
Degradation of anthraquinone dyes by Trametes trogii |
| title_full |
Degradation of anthraquinone dyes by Trametes trogii |
| title_fullStr |
Degradation of anthraquinone dyes by Trametes trogii |
| title_full_unstemmed |
Degradation of anthraquinone dyes by Trametes trogii |
| title_sort |
degradation of anthraquinone dyes by trametes trogii |
| url |
http://hdl.handle.net/20.500.12110/paper_03257541_v33_n4_p223_Levin |
| work_keys_str_mv |
AT levinl degradationofanthraquinonedyesbytrametestrogii AT jordana degradationofanthraquinonedyesbytrametestrogii AT forchiassinf degradationofanthraquinonedyesbytrametestrogii AT vialea degradationofanthraquinonedyesbytrametestrogii |
| _version_ |
1807316850268176384 |