Fungal metabolites with antiviral activity isolated from Curvularia pallescens

The strain BAFC 2336 of Curvularia pallescens is a hyphomycete isolated from internal fungi present in leaves and stems of Baccharis coridifolia. Three compounds designated B, D1 and D2 which inhibited the replication of vesicular stomatitis virus (VSV) and herpes simplex virus type 1 (HSV-1) in tis...

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Autores principales: Lemos, C., Couto, A.S., Bertoni, M.D., Coto, C.E.
Formato: JOUR
Materias:
Antiviral
Curvularia pallescens
Fungal metabolites
Herpes simplex virus type 1
Vesicular stomatitis virus
alpha,beta dehydrocurvularin
curvularia pallescens extract
natural product
unclassified drug
antiviral activity
article
curvularia pallescens
cytotoxicity
herpes simplex virus 1
high performance liquid chromatography
hyphomycetes
nonhuman
purification
thin layer chromatography
vero cell
vesicular stomatitis virus
Antiviral Agents
Mitosporic Fungi
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_03257541_v31_n4_p193_Lemos
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_03257541_v31_n4_p193_Lemos
record_format dspace
spelling todo:paper_03257541_v31_n4_p193_Lemos2023-10-03T15:24:11Z Fungal metabolites with antiviral activity isolated from Curvularia pallescens Lemos, C. Couto, A.S. Bertoni, M.D. Coto, C.E. Antiviral Curvularia pallescens Fungal metabolites Herpes simplex virus type 1 Vesicular stomatitis virus alpha,beta dehydrocurvularin curvularia pallescens extract natural product unclassified drug antiviral activity article curvularia pallescens cytotoxicity herpes simplex virus 1 high performance liquid chromatography hyphomycetes nonhuman purification thin layer chromatography vero cell vesicular stomatitis virus Antiviral Agents Mitosporic Fungi The strain BAFC 2336 of Curvularia pallescens is a hyphomycete isolated from internal fungi present in leaves and stems of Baccharis coridifolia. Three compounds designated B, D1 and D2 which inhibited the replication of vesicular stomatitis virus (VSV) and herpes simplex virus type 1 (HSV-1) in tissue cultures were isolated from fluid cultures of Curvularia pallescens. The purification procedure of the compounds consisted first in an organic solvent extraction followed by chromatography through a silica gel column. Fractions eluted from the column with antiviral activity were pooled and then subjected to thin layer chromatography (TLC) in silica gel plates. Three isolated bands were recognized with Rf of 0.63, 0.36 y 0.33 for B, D1 and D2, respectively. The chromatographic characteristics of the isolated metabolites were determined by TLC and HPLC and their chemical structure by means of spectroscopic methods. Analysis of the data obtained indicated that compound D2 (MW: 280 Da) is identical with Brefeldin A a macrolide with antiviral activity isolated from other fungi but not reported to be present in Curvularia pallescens. Compound D1 is similar in structure to compound D2; however, the low amount obtained after purification unabled us to obtain complete structural characterization. Compound B (MW: 332 Da) has an aromatic ring and a chemical structure related to curvularins, a generic name for certain metabolites from Curvularia. This compound appears to be a novel compound with antiviral potency similar to Brefeldin A, but less toxic. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_03257541_v31_n4_p193_Lemos
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Antiviral
Curvularia pallescens
Fungal metabolites
Herpes simplex virus type 1
Vesicular stomatitis virus
alpha,beta dehydrocurvularin
curvularia pallescens extract
natural product
unclassified drug
antiviral activity
article
curvularia pallescens
cytotoxicity
herpes simplex virus 1
high performance liquid chromatography
hyphomycetes
nonhuman
purification
thin layer chromatography
vero cell
vesicular stomatitis virus
Antiviral Agents
Mitosporic Fungi
spellingShingle Antiviral
Curvularia pallescens
Fungal metabolites
Herpes simplex virus type 1
Vesicular stomatitis virus
alpha,beta dehydrocurvularin
curvularia pallescens extract
natural product
unclassified drug
antiviral activity
article
curvularia pallescens
cytotoxicity
herpes simplex virus 1
high performance liquid chromatography
hyphomycetes
nonhuman
purification
thin layer chromatography
vero cell
vesicular stomatitis virus
Antiviral Agents
Mitosporic Fungi
Lemos, C.
Couto, A.S.
Bertoni, M.D.
Coto, C.E.
Fungal metabolites with antiviral activity isolated from Curvularia pallescens
topic_facet Antiviral
Curvularia pallescens
Fungal metabolites
Herpes simplex virus type 1
Vesicular stomatitis virus
alpha,beta dehydrocurvularin
curvularia pallescens extract
natural product
unclassified drug
antiviral activity
article
curvularia pallescens
cytotoxicity
herpes simplex virus 1
high performance liquid chromatography
hyphomycetes
nonhuman
purification
thin layer chromatography
vero cell
vesicular stomatitis virus
Antiviral Agents
Mitosporic Fungi
description The strain BAFC 2336 of Curvularia pallescens is a hyphomycete isolated from internal fungi present in leaves and stems of Baccharis coridifolia. Three compounds designated B, D1 and D2 which inhibited the replication of vesicular stomatitis virus (VSV) and herpes simplex virus type 1 (HSV-1) in tissue cultures were isolated from fluid cultures of Curvularia pallescens. The purification procedure of the compounds consisted first in an organic solvent extraction followed by chromatography through a silica gel column. Fractions eluted from the column with antiviral activity were pooled and then subjected to thin layer chromatography (TLC) in silica gel plates. Three isolated bands were recognized with Rf of 0.63, 0.36 y 0.33 for B, D1 and D2, respectively. The chromatographic characteristics of the isolated metabolites were determined by TLC and HPLC and their chemical structure by means of spectroscopic methods. Analysis of the data obtained indicated that compound D2 (MW: 280 Da) is identical with Brefeldin A a macrolide with antiviral activity isolated from other fungi but not reported to be present in Curvularia pallescens. Compound D1 is similar in structure to compound D2; however, the low amount obtained after purification unabled us to obtain complete structural characterization. Compound B (MW: 332 Da) has an aromatic ring and a chemical structure related to curvularins, a generic name for certain metabolites from Curvularia. This compound appears to be a novel compound with antiviral potency similar to Brefeldin A, but less toxic.
format JOUR
author Lemos, C.
Couto, A.S.
Bertoni, M.D.
Coto, C.E.
author_facet Lemos, C.
Couto, A.S.
Bertoni, M.D.
Coto, C.E.
author_sort Lemos, C.
title Fungal metabolites with antiviral activity isolated from Curvularia pallescens
title_short Fungal metabolites with antiviral activity isolated from Curvularia pallescens
title_full Fungal metabolites with antiviral activity isolated from Curvularia pallescens
title_fullStr Fungal metabolites with antiviral activity isolated from Curvularia pallescens
title_full_unstemmed Fungal metabolites with antiviral activity isolated from Curvularia pallescens
title_sort fungal metabolites with antiviral activity isolated from curvularia pallescens
url http://hdl.handle.net/20.500.12110/paper_03257541_v31_n4_p193_Lemos
work_keys_str_mv AT lemosc fungalmetaboliteswithantiviralactivityisolatedfromcurvulariapallescens
AT coutoas fungalmetaboliteswithantiviralactivityisolatedfromcurvulariapallescens
AT bertonimd fungalmetaboliteswithantiviralactivityisolatedfromcurvulariapallescens
AT cotoce fungalmetaboliteswithantiviralactivityisolatedfromcurvulariapallescens
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