Inhibition of Junin virus multiplication by caffeine

The inhibitory activity of caffeine, a pharmacologically active compound, on Junin virus (JV) multiplication in Veto cells was evaluated by a virus yield inhibition assay. The compound achieved a dose-dependent inhibition of virus production at concentrations not affecting cell viability. The 50% in...

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Autores principales:	Candurra, N.A., Damonte, E.B.
Formato:	JOUR
Materias:	Antiviral activity Arenaviruses Argentine hemorrhagic fever Caffeine Junin virus caffeine virus protein antivirus agent animal cell antiviral activity article dose response immunofluorescence junin virus nonhuman protein expression vero cell virogenesis animal biosynthesis Cercopithecus drug effect fluorescent antibody technique physiology time Vero cell virus replication Animals Antiviral Agents Cercopithecus aethiops Dose-Response Relationship, Drug Fluorescent Antibody Technique, Indirect Time Factors Vero Cells Viral Proteins Virus Replication
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_03257541_v31_n3_p135_Candurra
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_03257541_v31_n3_p135_Candurra
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spelling	todo:paper_03257541_v31_n3_p135_Candurra2023-10-03T15:24:10Z Inhibition of Junin virus multiplication by caffeine Candurra, N.A. Damonte, E.B. Antiviral activity Arenaviruses Argentine hemorrhagic fever Caffeine Junin virus caffeine virus protein antivirus agent caffeine animal cell antiviral activity article dose response immunofluorescence junin virus nonhuman protein expression vero cell virogenesis animal biosynthesis Cercopithecus drug effect fluorescent antibody technique Junin virus physiology time Vero cell virus replication Animals Antiviral Agents Caffeine Cercopithecus aethiops Dose-Response Relationship, Drug Fluorescent Antibody Technique, Indirect Junin virus Time Factors Vero Cells Viral Proteins Virus Replication The inhibitory activity of caffeine, a pharmacologically active compound, on Junin virus (JV) multiplication in Veto cells was evaluated by a virus yield inhibition assay. The compound achieved a dose-dependent inhibition of virus production at concentrations not affecting cell viability. The 50% inhibitory concentration (IC50) and the 90% inhibitory concentration (IC90) were 9.0 mM and 10.8 mM, respectively. From time of addition experiments, it can be concluded that caffeine inhibited an early stage in the replicative cycle of JV occurring before 4 h of infection. Extracellular and cell-associated virus yields were reduced to the same extent. The addition of caffeine after several cycles of infection for a very short treatment period did not significantly affect the formation of JV infectious particles. The expression of viral proteins in the cytoplasm and the membrane of infected cells was highly reduced in the presence of caffeine, as revealed by immunofluorescence staining, confirming that caffeine predominantly exerted its inhibitory action early in the infection of Vero cells with JV. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_03257541_v31_n3_p135_Candurra
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Antiviral activity Arenaviruses Argentine hemorrhagic fever Caffeine Junin virus caffeine virus protein antivirus agent caffeine animal cell antiviral activity article dose response immunofluorescence junin virus nonhuman protein expression vero cell virogenesis animal biosynthesis Cercopithecus drug effect fluorescent antibody technique Junin virus physiology time Vero cell virus replication Animals Antiviral Agents Caffeine Cercopithecus aethiops Dose-Response Relationship, Drug Fluorescent Antibody Technique, Indirect Junin virus Time Factors Vero Cells Viral Proteins Virus Replication
spellingShingle	Antiviral activity Arenaviruses Argentine hemorrhagic fever Caffeine Junin virus caffeine virus protein antivirus agent caffeine animal cell antiviral activity article dose response immunofluorescence junin virus nonhuman protein expression vero cell virogenesis animal biosynthesis Cercopithecus drug effect fluorescent antibody technique Junin virus physiology time Vero cell virus replication Animals Antiviral Agents Caffeine Cercopithecus aethiops Dose-Response Relationship, Drug Fluorescent Antibody Technique, Indirect Junin virus Time Factors Vero Cells Viral Proteins Virus Replication Candurra, N.A. Damonte, E.B. Inhibition of Junin virus multiplication by caffeine
topic_facet	Antiviral activity Arenaviruses Argentine hemorrhagic fever Caffeine Junin virus caffeine virus protein antivirus agent caffeine animal cell antiviral activity article dose response immunofluorescence junin virus nonhuman protein expression vero cell virogenesis animal biosynthesis Cercopithecus drug effect fluorescent antibody technique Junin virus physiology time Vero cell virus replication Animals Antiviral Agents Caffeine Cercopithecus aethiops Dose-Response Relationship, Drug Fluorescent Antibody Technique, Indirect Junin virus Time Factors Vero Cells Viral Proteins Virus Replication
description	The inhibitory activity of caffeine, a pharmacologically active compound, on Junin virus (JV) multiplication in Veto cells was evaluated by a virus yield inhibition assay. The compound achieved a dose-dependent inhibition of virus production at concentrations not affecting cell viability. The 50% inhibitory concentration (IC50) and the 90% inhibitory concentration (IC90) were 9.0 mM and 10.8 mM, respectively. From time of addition experiments, it can be concluded that caffeine inhibited an early stage in the replicative cycle of JV occurring before 4 h of infection. Extracellular and cell-associated virus yields were reduced to the same extent. The addition of caffeine after several cycles of infection for a very short treatment period did not significantly affect the formation of JV infectious particles. The expression of viral proteins in the cytoplasm and the membrane of infected cells was highly reduced in the presence of caffeine, as revealed by immunofluorescence staining, confirming that caffeine predominantly exerted its inhibitory action early in the infection of Vero cells with JV.
format	JOUR
author	Candurra, N.A. Damonte, E.B.
author_facet	Candurra, N.A. Damonte, E.B.
author_sort	Candurra, N.A.
title	Inhibition of Junin virus multiplication by caffeine
title_short	Inhibition of Junin virus multiplication by caffeine
title_full	Inhibition of Junin virus multiplication by caffeine
title_fullStr	Inhibition of Junin virus multiplication by caffeine
title_full_unstemmed	Inhibition of Junin virus multiplication by caffeine
title_sort	inhibition of junin virus multiplication by caffeine
url	http://hdl.handle.net/20.500.12110/paper_03257541_v31_n3_p135_Candurra
work_keys_str_mv	AT candurrana inhibitionofjuninvirusmultiplicationbycaffeine AT damonteeb inhibitionofjuninvirusmultiplicationbycaffeine
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Inhibition of Junin virus multiplication by caffeine

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