Influence of different cultural conditions on cellulase production by Nectria catalinensis.
The production of the extracellular cellulolytic enzyme system (endoglucanase, exoglucanase and cellobiase) of N. catalinensis was tested with different nitrogen sources, inorganic and organic ones, in liquid culture medium with microcrystalline cellulose. The nitrogen compounds used were: potassium...
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todo:paper_03257541_v30_n1_p20_Pardo2023-10-03T15:24:07Z Influence of different cultural conditions on cellulase production by Nectria catalinensis. Pardo, A.G. Forchiassin, F. amino acid cellulase cellulose fungal protein glycogen nitrate nitrogen quaternary ammonium derivative urea article Ascomycetes biosynthesis cell wall comparative study culture medium drug effect enzyme induction enzymology fermentation gene expression regulation growth, development and aging metabolism methodology mycology pH temperature Amino Acids Cell Wall Cellulase Cellulose Culture Media Enzyme Induction Fermentation Fungal Proteins Gene Expression Regulation, Fungal Glycogen Hydrogen-Ion Concentration Hypocreales Mycology Nitrates Nitrogen Quaternary Ammonium Compounds Temperature Urea The production of the extracellular cellulolytic enzyme system (endoglucanase, exoglucanase and cellobiase) of N. catalinensis was tested with different nitrogen sources, inorganic and organic ones, in liquid culture medium with microcrystalline cellulose. The nitrogen compounds used were: potassium nitrate, sodium nitrate, ammonium nitrate, ammonium phosphate, ammonium sulphate, ammonium chloride, ammonium carbonate, ammonium acetate, ammonium tartrate, urea, casamino acids, glycine, L-alanine, L-leucine, L-proline, L-lysine, L-aspartic acid, L-glutamic acid, L-asparagine, L-glutamine, L-phenylalanine, L-tyrosine, L-tryptophan, L-methionine and L-cysteine. Among these, ammonium nitrate and ammonium tartrate gave the highest yields of cellulases in 20-day-old cultures at a concentration equivalent to 0.75 g N/l in both cases. Optimal temperature for cellulase production, growth and cellulose degradation was 23 degrees C. On the other hand, an initial pH of 6.5 gave the highest yields of endoglucanase and cellobiase. In the same way, at pH 6.5, maximal growth and cellulose degradation were achieved. However, maximal exoglucanase production and glycogen content were reached at pH 7.5. Fil:Pardo, A.G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Forchiassin, F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_03257541_v30_n1_p20_Pardo |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
amino acid cellulase cellulose fungal protein glycogen nitrate nitrogen quaternary ammonium derivative urea article Ascomycetes biosynthesis cell wall comparative study culture medium drug effect enzyme induction enzymology fermentation gene expression regulation growth, development and aging metabolism methodology mycology pH temperature Amino Acids Cell Wall Cellulase Cellulose Culture Media Enzyme Induction Fermentation Fungal Proteins Gene Expression Regulation, Fungal Glycogen Hydrogen-Ion Concentration Hypocreales Mycology Nitrates Nitrogen Quaternary Ammonium Compounds Temperature Urea |
| spellingShingle |
amino acid cellulase cellulose fungal protein glycogen nitrate nitrogen quaternary ammonium derivative urea article Ascomycetes biosynthesis cell wall comparative study culture medium drug effect enzyme induction enzymology fermentation gene expression regulation growth, development and aging metabolism methodology mycology pH temperature Amino Acids Cell Wall Cellulase Cellulose Culture Media Enzyme Induction Fermentation Fungal Proteins Gene Expression Regulation, Fungal Glycogen Hydrogen-Ion Concentration Hypocreales Mycology Nitrates Nitrogen Quaternary Ammonium Compounds Temperature Urea Pardo, A.G. Forchiassin, F. Influence of different cultural conditions on cellulase production by Nectria catalinensis. |
| topic_facet |
amino acid cellulase cellulose fungal protein glycogen nitrate nitrogen quaternary ammonium derivative urea article Ascomycetes biosynthesis cell wall comparative study culture medium drug effect enzyme induction enzymology fermentation gene expression regulation growth, development and aging metabolism methodology mycology pH temperature Amino Acids Cell Wall Cellulase Cellulose Culture Media Enzyme Induction Fermentation Fungal Proteins Gene Expression Regulation, Fungal Glycogen Hydrogen-Ion Concentration Hypocreales Mycology Nitrates Nitrogen Quaternary Ammonium Compounds Temperature Urea |
| description |
The production of the extracellular cellulolytic enzyme system (endoglucanase, exoglucanase and cellobiase) of N. catalinensis was tested with different nitrogen sources, inorganic and organic ones, in liquid culture medium with microcrystalline cellulose. The nitrogen compounds used were: potassium nitrate, sodium nitrate, ammonium nitrate, ammonium phosphate, ammonium sulphate, ammonium chloride, ammonium carbonate, ammonium acetate, ammonium tartrate, urea, casamino acids, glycine, L-alanine, L-leucine, L-proline, L-lysine, L-aspartic acid, L-glutamic acid, L-asparagine, L-glutamine, L-phenylalanine, L-tyrosine, L-tryptophan, L-methionine and L-cysteine. Among these, ammonium nitrate and ammonium tartrate gave the highest yields of cellulases in 20-day-old cultures at a concentration equivalent to 0.75 g N/l in both cases. Optimal temperature for cellulase production, growth and cellulose degradation was 23 degrees C. On the other hand, an initial pH of 6.5 gave the highest yields of endoglucanase and cellobiase. In the same way, at pH 6.5, maximal growth and cellulose degradation were achieved. However, maximal exoglucanase production and glycogen content were reached at pH 7.5. |
| format |
JOUR |
| author |
Pardo, A.G. Forchiassin, F. |
| author_facet |
Pardo, A.G. Forchiassin, F. |
| author_sort |
Pardo, A.G. |
| title |
Influence of different cultural conditions on cellulase production by Nectria catalinensis. |
| title_short |
Influence of different cultural conditions on cellulase production by Nectria catalinensis. |
| title_full |
Influence of different cultural conditions on cellulase production by Nectria catalinensis. |
| title_fullStr |
Influence of different cultural conditions on cellulase production by Nectria catalinensis. |
| title_full_unstemmed |
Influence of different cultural conditions on cellulase production by Nectria catalinensis. |
| title_sort |
influence of different cultural conditions on cellulase production by nectria catalinensis. |
| url |
http://hdl.handle.net/20.500.12110/paper_03257541_v30_n1_p20_Pardo |
| work_keys_str_mv |
AT pardoag influenceofdifferentculturalconditionsoncellulaseproductionbynectriacatalinensis AT forchiassinf influenceofdifferentculturalconditionsoncellulaseproductionbynectriacatalinensis |
| _version_ |
1807324360733622272 |