Poly-(3-hydroxybutyrate) granules in Bacillus megaterium. Isolation and analysis of associated proteins

Bacillus megaterium accumulates poly-(3-hydroxybutyrate) (PHB) as a reserve material in intracellular granules. In this work we described a method for the preparation of PHB granules from B. megaterium PV447 and the analysis by polyacrylamide gel electrophoresis of the associated proteins. By compar...

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Detalles Bibliográficos
Autores principales:	Vázquez, G.J., Wieczoreck, R., Steinbüchel, A., Méndez, B.S.
Formato:	JOUR
Materias:	acyltransferase bacterial protein hydroxybutyric acid lectin phasin plant lectin poly(3 hydroxybutyric acid) poly(3-hydroxyalkanoic acid) synthase poly-beta-hydroxybutyrate polyester polyhydroxyalkanoate synthase article Bacillus megaterium cell fractionation cell granule chemistry Chromatium comparative study enzymology isolation and purification polyacrylamide gel electrophoresis species difference ultrastructure Acyltransferases Bacterial Proteins Cell Fractionation Cytoplasmic Granules Electrophoresis, Polyacrylamide Gel Hydroxybutyrates Lectins Plant Lectins Polyesters Species Specificity
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_03257541_v28_n3_p118_Vazquez
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_03257541_v28_n3_p118_Vazquez
record_format	dspace
spelling	todo:paper_03257541_v28_n3_p118_Vazquez2023-10-03T15:24:04Z Poly-(3-hydroxybutyrate) granules in Bacillus megaterium. Isolation and analysis of associated proteins Vázquez, G.J. Wieczoreck, R. Steinbüchel, A. Méndez, B.S. acyltransferase bacterial protein hydroxybutyric acid lectin phasin plant lectin poly(3 hydroxybutyric acid) poly(3-hydroxyalkanoic acid) synthase poly-beta-hydroxybutyrate polyester polyhydroxyalkanoate synthase article Bacillus megaterium cell fractionation cell granule chemistry Chromatium comparative study enzymology isolation and purification polyacrylamide gel electrophoresis species difference ultrastructure Acyltransferases Bacillus megaterium Bacterial Proteins Cell Fractionation Chromatium Cytoplasmic Granules Electrophoresis, Polyacrylamide Gel Hydroxybutyrates Lectins Plant Lectins Polyesters Species Specificity Bacillus megaterium accumulates poly-(3-hydroxybutyrate) (PHB) as a reserve material in intracellular granules. In this work we described a method for the preparation of PHB granules from B. megaterium PV447 and the analysis by polyacrylamide gel electrophoresis of the associated proteins. By comparison with another species a function is proposed for some of these proteins. Fil:Vázquez, G.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Méndez, B.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_03257541_v28_n3_p118_Vazquez
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	acyltransferase bacterial protein hydroxybutyric acid lectin phasin plant lectin poly(3 hydroxybutyric acid) poly(3-hydroxyalkanoic acid) synthase poly-beta-hydroxybutyrate polyester polyhydroxyalkanoate synthase article Bacillus megaterium cell fractionation cell granule chemistry Chromatium comparative study enzymology isolation and purification polyacrylamide gel electrophoresis species difference ultrastructure Acyltransferases Bacillus megaterium Bacterial Proteins Cell Fractionation Chromatium Cytoplasmic Granules Electrophoresis, Polyacrylamide Gel Hydroxybutyrates Lectins Plant Lectins Polyesters Species Specificity
spellingShingle	acyltransferase bacterial protein hydroxybutyric acid lectin phasin plant lectin poly(3 hydroxybutyric acid) poly(3-hydroxyalkanoic acid) synthase poly-beta-hydroxybutyrate polyester polyhydroxyalkanoate synthase article Bacillus megaterium cell fractionation cell granule chemistry Chromatium comparative study enzymology isolation and purification polyacrylamide gel electrophoresis species difference ultrastructure Acyltransferases Bacillus megaterium Bacterial Proteins Cell Fractionation Chromatium Cytoplasmic Granules Electrophoresis, Polyacrylamide Gel Hydroxybutyrates Lectins Plant Lectins Polyesters Species Specificity Vázquez, G.J. Wieczoreck, R. Steinbüchel, A. Méndez, B.S. Poly-(3-hydroxybutyrate) granules in Bacillus megaterium. Isolation and analysis of associated proteins
topic_facet	acyltransferase bacterial protein hydroxybutyric acid lectin phasin plant lectin poly(3 hydroxybutyric acid) poly(3-hydroxyalkanoic acid) synthase poly-beta-hydroxybutyrate polyester polyhydroxyalkanoate synthase article Bacillus megaterium cell fractionation cell granule chemistry Chromatium comparative study enzymology isolation and purification polyacrylamide gel electrophoresis species difference ultrastructure Acyltransferases Bacillus megaterium Bacterial Proteins Cell Fractionation Chromatium Cytoplasmic Granules Electrophoresis, Polyacrylamide Gel Hydroxybutyrates Lectins Plant Lectins Polyesters Species Specificity
description	Bacillus megaterium accumulates poly-(3-hydroxybutyrate) (PHB) as a reserve material in intracellular granules. In this work we described a method for the preparation of PHB granules from B. megaterium PV447 and the analysis by polyacrylamide gel electrophoresis of the associated proteins. By comparison with another species a function is proposed for some of these proteins.
format	JOUR
author	Vázquez, G.J. Wieczoreck, R. Steinbüchel, A. Méndez, B.S.
author_facet	Vázquez, G.J. Wieczoreck, R. Steinbüchel, A. Méndez, B.S.
author_sort	Vázquez, G.J.
title	Poly-(3-hydroxybutyrate) granules in Bacillus megaterium. Isolation and analysis of associated proteins
title_short	Poly-(3-hydroxybutyrate) granules in Bacillus megaterium. Isolation and analysis of associated proteins
title_full	Poly-(3-hydroxybutyrate) granules in Bacillus megaterium. Isolation and analysis of associated proteins
title_fullStr	Poly-(3-hydroxybutyrate) granules in Bacillus megaterium. Isolation and analysis of associated proteins
title_full_unstemmed	Poly-(3-hydroxybutyrate) granules in Bacillus megaterium. Isolation and analysis of associated proteins
title_sort	poly-(3-hydroxybutyrate) granules in bacillus megaterium. isolation and analysis of associated proteins
url	http://hdl.handle.net/20.500.12110/paper_03257541_v28_n3_p118_Vazquez
work_keys_str_mv	AT vazquezgj poly3hydroxybutyrategranulesinbacillusmegateriumisolationandanalysisofassociatedproteins AT wieczoreckr poly3hydroxybutyrategranulesinbacillusmegateriumisolationandanalysisofassociatedproteins AT steinbuchela poly3hydroxybutyrategranulesinbacillusmegateriumisolationandanalysisofassociatedproteins AT mendezbs poly3hydroxybutyrategranulesinbacillusmegateriumisolationandanalysisofassociatedproteins
_version_	1807318821225103360

Poly-(3-hydroxybutyrate) granules in Bacillus megaterium. Isolation and analysis of associated proteins

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